Anti-CRISPR Cas9 Antibody (6F7)
Our Anti-CRISPR Cas9 primary antibody from PhosphoSolutions is mouse monoclonal. It detects CRISPR Cas9 and is Protein G purified. It is great for use in WB, IHC, ICC.
SKU: 304-cas9
Product Details
CRISPR Cas9
CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes. Antibodies against Cas9 are very useful in the direct detection of the CRISPR/Cas9 complex. This antibody detects Cas9 from Staphylococcus aureus transfected into cells and shows no reactivity toward the Streptococcus pyogenes Cas9 protein (Ran et al., 2015).
Protein G Purified
Monoclonal
6F7
IgG1
ICC, IHC, WB
Mouse
53 kDa
Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.
AB_2715491
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Protein G purified culture supernatant.
PBS + 50% glycerol and 5 mm NaN3
WB: 1:1000
IHC: 1:1000-1:5000
ICC: 1:500-1:5000
IHC: 1:1000-1:5000
ICC: 1:500-1:5000
Unconjugated
Specific for the Staphylococcus aureus Cas9 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Cas9 antibody, CRISPR-associated endonuclease Cas9/Csn1 antibody, CRISPR-Cas9/Csn1 antibody, csn1 antibody, SpyCas9 antibody
Product Specific Protocols
- Western Blot Protocol: Download
General References
- Ran, F.A., Cong, L., Yan, W.X., Scott, D.A., Gootenberg, J.S., Kriz, A.J., Zetsche, B., Shalem, O., Wu, X., Makarova, K.S. and Koonin, E.V., 2015. In vivo genome editing using Staphylococcus aureus Cas9. Nature, 520(7546), p.186. PMID: 25830891