Anti-CRMP2 (Ser-522), Phosphospecific Antibody

26 Citations

Our CRMP2 (Ser-522) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat CRMP2 (Ser-522) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot image of mouse brain untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) (lanes 1 & 2) or anti-CRMP2 (Ser-522) (lanes 3 & 4).
Western blot image of mouse brain untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) (lanes 1 & 2) or anti-CRMP2 (Ser-522) (lanes 3 & 4).
Roll over image to zoom in Click on image to zoom
Western blot image of mouse brain untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) (lanes 1 & 2) or anti-CRMP2 (Ser-522) (lanes 3 & 4).
Immunocytochemical labeling of CRMP2 phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-CRMP2 (Ser-522) (CP2191) antibody in the absence (Left) or presence (Right) of blocking peptide (CX2195). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Human, Mouse, Rat
ICC, WB
Rabbit

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Data Sheet

SKU: CP2191

Volume: 100 µL
Price:
$340.00
Ships: 1-2 business days

Product Details

CRMP2 (Ser-522)
CRMP2 (CRMP-62, TOAD-64, DRP-2) is a microtubule associated protein involved in neuron development and axon pathfinding. CRMP2 binds to tubulin heterodimers and promotes microtubule assembly. The overexpression of CRMP2 facilitates the rate of axonal growth, whereas the mutated form that lacks activity toward the microtubule assembly inhibits axonal growth in a dominant negative manner. Phosphorylation of CRMP2 regulates its activity and this type of regulation has been implicated in axon growth cone collapse induced by several repulsive cues. Cdk5 and GSK3 phosphorylation occurs downstream of the repulsive cue, Sema-3A. Several residues in CRMP2 are phosphorylated by GSK3 (Ser-518,Thr-514, and Thr-509), and a priming site (Ser-522). These sites are conserved in human CRMP1 and CRMP4, but not in CRMP3 or CRMP5. The priming site is also phosphorylated by Cdk5. In contrast, ROCK phosphorylates Thr-555 leading to LPA, MAG, or Ephrin-A5 mediated growth cone collapse. Thus, CRMP2 phosphorylation status may be a critical element of pathways that control axon pathfinding.
Antigen Affinity Purified
Polyclonal
IgG
ICC, WB
Rabbit
DPYSL2
70
Phospho-CRMP2 (Ser-522) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-522 in human CRMP2. This sequence is conserved in rat and mouse CRMP2, and has homology to the conserved site in CRMP1 and CRMP4, but is not conserved in CRMP3 and CRMP5.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:100
Unconjugated
The antibody detects a 70 kDa* protein corresponding to the molecular mass of CRMP2 on SDS-PAGE immunoblots of human mouse brain, and this band is removed after lambda phosphatase treatment. In addition, this reactivity can be specifically blocked using phospho-CRMP2 (Ser-522) peptide (CX2195).
Phosphorylated
Ser-522
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
DRP-2, Toad-64, CRMP-62
Q16555

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
33401710not listedPaik, S, et al. 2021. Somatostatin Ameliorates β-Amyloid-Induced Cytotoxicity via the Regulation of CRMP2 Phosphorylation and Calcium Homeostasis in SH-SY5Y Cells. Biomedicines, 27.
Immunocytochemistry: Rat
PMID Dilution Publication
28809766not listedMoutal, A, et al. 2017. CRISPR/Cas9 editing of Nf1 gene identifies CRMP2 as a therapeutic target in neurofibromatosis type 1-related pain that is reversed by (S)-Lacosamide. Pain, 2301-2319.
Immunohistochemistry: Mouse
PMID Dilution Publication
342978161:200Yamazaki, Y, et al. 2022. Requirement of CRMP2 Phosphorylation in Neuronal Migration of Developing Mouse Cerebral Cortex and Hippocampus and Redundant Roles of CRMP1 and …. Cerebral cortex, 520-527.
329732421:1000Nishijima, E, et al. 2020. Topical ripasudil stimulates neuroprotection and axon regeneration in adult mice following optic nerve injury. Scientific reports, 15709.
321565711:200Yamazaki, Y, et al. 2020. Phosphorylation of CRMP2 is required for migration and positioning of Purkinje cells: Redundant roles of CRMP1 and CRMP4. Brain research, 146762.
303751271:200Togashi, K, et al. 2019. Genetic suppression of collapsin response mediator protein 2 phosphorylation improves outcome in methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson’s model mice. Genes to cells : devoted to molecular & cellular mechanisms, 31-40.
28660485not listedMoutal, A, et al. 2018. CRMP2 Phosphorylation Drives Glioblastoma Cell Proliferation. Molecular neurobiology, 4403-4416.
Immunohistochemistry: Rat
PMID Dilution Publication
32571373not listedMoutal, A, et al. 2020. Differential expression of Cdk5-phosphorylated CRMP2 following a spared nerve injury. Molecular brain, 97.
27917413not listedMoutal, A, et al. 2016. Efficacy of ( S)-Lacosamide in preclinical models of cephalic pain. Pain reports, e565.
Western Blot: Human
PMID Dilution Publication
334017101:1000Paik, S, et al. 2021. Somatostatin Ameliorates β-Amyloid-Induced Cytotoxicity via the Regulation of CRMP2 Phosphorylation and Calcium Homeostasis in SH-SY5Y Cells. Biomedicines, 17.
30816227not listedDuciel, L, et al. 2019. Protein tyrosine phosphatase 4A3 (PTP4A3/PRL-3) promotes the aggressiveness of human uveal melanoma through dephosphorylation of CRMP2. Scientific reports, 2990.
299268351:2000Mokhtar, S, et al. 2018. Amyloid-beta-dependent phosphorylation of collapsin response mediator protein-2 dissociates kinesin in Alzheimer's disease. Neural regeneration research, 1066-1080.
Western Blot: Mouse
PMID Dilution Publication
371746871:1500Brustovetsky, T, et al. 2023. CRMP2 Participates in Regulating Mitochondrial Morphology and Motility in Alzheimer's Disease. Cells, 1287.
355899151:1000Yazawa, A, et al. 2022. Effects of Lanthionine Ketimine-5-Ethyl Ester on the α-Synucleinopathy Mouse Model. Neurochemical Research, 2373-2382.
348313951:1500Brustovestsky, T, et al. 2021. Involvement of CRMP2 in Regulation of Mitochondrial Morphology and Motility in Huntington's Disease. Cells, 3172.
346857601:1500Brustovestsky, T, et al. 2021. CRMP2 Is Involved in Regulation of Mitochondrial Morphology and Motility in Neurons. Cells, 2781.
31795726not listedMoutal, A, et al. 2019. Neuronal Conditional Knockout of Collapsin Response Mediator Protein 2 Ameliorates Disease Severity in a Mouse Model of Multiple Sclerosis. ASN Neuro, 44943.
28648892not listedLiu, R, et al. 2017. ASCIZ/ATMIN is dispensable for ATM signaling in response to replication stress. DNA repair, 29-34.
Western Blot: Rat
PMID Dilution Publication
396627921:2000Su, Z, et al. 2024. CRMP2 regulates mossy fiber sprouting and modulates microtubule dynamics in a pilocarpine induced rat model of epilepsy. Brain Research, 149395.
384716251:2500Kim, K, et al. 2024. Insular cortex stimulation alleviates neuropathic pain through changes in the expression of collapsin response mediator protein 2 involved in synaptic plasticity. Neurobiology of Disease, 106466.
353344121:1000Li, Y, et al. 2022. CRMP2 Modulates Mossy Fiber Sprouting in Dentate Gyrus of Pilocarpine Induced Rat Model of Epilepsy. Biochemical and Biophysical Research Communications, 141-147.
365648531:1000Jiang, YP, et al. 2022. Neuronal CRMP2 phosphorylation inhibition by the flavonoid, naringenin, contributes to the reversal of spinal sensitization and arthritic pain improvement. Arthritis research & therapy, 277.
31680630not listedMoutal, A, et al. 2019. Evaluation of edonerpic maleate as a CRMP2 inhibitor for pain relief. Channels (Austin, Tex.), 498-504.
31080913not listedMoutal, A, et al. 2019. Cdk5-mediated CRMP2 phosphorylation is necessary and sufficient for peripheral neuropathic pain. Neurobiology of pain (Cambridge, Mass.), 100022.
28767512not listedMoutal, A, et al. 2017. Dissecting the role of the CRMP2-neurofibromin complex on pain behaviors. Pain, 2203-2221.
26967696not listedMoutal, A, et al. 2016. (S)-lacosamide inhibition of CRMP2 phosphorylation reduces postoperative and neuropathic pain behaviors through distinct classes of sensory neurons identified by constellation pharmacology. Pain reports, 1448-1463.

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