Our Anti-Cytokeratin 18 (CK18) mouse monoclonal primary antibody from Antibodies Incorporated is produced in-house from hybridoma clone CK 209. It detects human Cytokeratin 18 (CK18), and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB, ELISA.
Immunofluorescent staining of MCF7 cells using 1 ug/mL Antibodies Incorporated mouse monoclonal anti-Cytokeratin-18 (CK18) antibody (MM-1009) (green). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat#AR-6501). DAPI nuclear stain (blue) shows cell nuclei. Anti-CK18 stains the cytoskeleton of MCF7 cells.
Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.GI tract contain both CK 8 and CK18 but not CK 14. Cytokeratin is expressed in GI and respiratory tract, mesothelial cells, endocrine and exocrine tissues. Varieties of simple epithelia including glandular but not stratified squamous epithelia are detected by this antibody.
Purified by Protein A Chromatography
1 mg/mL
Monoclonal
CK 209
IgG1
ELISA, ICC, IHC, WB
Mouse
KRT18
48 kDa
Human breast cancer PMC 42 cells
Human
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
