Anti-Cytokeratin 19 (CK19) Antibody (CK 202)

Name: Anti-Cytokeratin 19 (CK19) Antibody
Brand: Antibodies Incorporated
SKU: MM-1002
Price: 329.0 USD
Availability: InStock

Our Anti-Cytokeratin 19 (CK19) mouse monoclonal primary antibody from Antibodies Incorporated is produced in-house from hybridoma clone CK 202. It detects human Cytokeratin 19 (CK19), and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB, ELISA.

Western blotting of HepG2 cell lysate using 0.2 ug/mL, 1 ug/mL, or 5 ug/mL mouse anti-Cytokeratin-19 antibody (MM-1002). MM-1002 mouse anti-CK19 antibody recognizes endogenous CK19 at ~40 kDa.

Immunofluorescent staining of MCF7 cells using 10 ug/mL mouse monoclonal anti-Cytokeratin-19 (CK19) antibody (MM-1002) (green). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat#AR-6501). DAPI nuclear stain (blue) shows cell nuclei. Anti-CK19 stains the cytoskeleton of MCF7 cells.

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Species Reactivity

Human

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: MM-1002

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Product Details

Target

Cytokeratin 19 (CK19)

Target Description

Cytokeratin 19 is a type I acidic subfamily of intermediate filament protein that is expressed in stratified and simple type epithelia. Keratin 19 is not expressed in hepatocytes; therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumors. Keratin 19 is often co-expressed with keratin 7.

Format

Purified by Protein A Chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

CK 202

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

KRT19

Molecular Weight

40 kDa

Antigen

Human mammary epithelium, solubilized by detergent.

Species Reactivity

Human

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000 -1:5000
IHC: 1:100
ICC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

K19; CK19; K1CS, Cytokeratin-19

UniProt ID

P08727

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Anti-Cytokeratin 19 (CK19) Antibody (CK 202)

SKU: MM-1002

Product Details

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