Our Anti-DENND3 (Ser554) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human DENND3 (Ser554) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of HeLa cell lysate showing specific immunolabeling of the ~142 kDa DENND3 protein phosphorylated at Ser554 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).
The DENN (differentially expressed in normal and neoplastic cells) domain (DENND) is a poorly characterized protein module conserved throughout evolution (Marat, A.L., et al., 2011). Proteins bearing a DENN domain have recently emerged as the largest family of Rab GEFs. Among these DENN domain proteins, DENND3 is a GEF for Rab12; promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (Xu and McPherson, 2015; Matsui et al., 2011). ULK-mediated phosphorylation of DENND3 at serines 554 and 572 upregulates its GEF activity toward the small GTPase Rab12 (Xu and McPherson, 2015).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
DENND3
142 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser554 of human DENND3, conjugated to keyhole limpet hemocyanin (KLH).
Human
Non-Human Primate, Mouse, Rat, Sheep
AB_2560942
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phospho peptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:500
Unconjugated
Specific for endogenous levels of the ~142 kDa DENND3 phosphorylated at Ser554. The immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Ser554
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
DENN Domain Containing 3, DENN Domain-Containing Protein 3, DENN/MADD Domain Containing 3, KIAA0870 DENN/MADD domain containing 3, KIAA0870DENN domain-containing protein 3
Xu, J. and McPherson, P.S., 2015. DENND3: a signaling/trafficking interface in autophagy. Cell Cycle, 14(17), p.2717. PMID: 26177209
Matsui, T., Itoh, T. and Fukuda, M., 2011. Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic, 12(10), pp.1432-1443. PMID: 21718402
Marat, A.L., Dokainish, H. and McPherson, P.S., 2011. DENN domain proteins: regulators of Rab GTPases. Journal of Biological Chemistry, 286(16), pp.13791-13800. PMID: 21330364
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