Anti-Doublecortin Antibody (3E1 )
Our Anti-Doublecortin primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, and rat Doublecortin and is Protein G purified. It is great for use in WB, IHC, ICC.
![Immunofluorescence of rat cortical neuron-glial cells showing strong cytoplasmic labeling of a small population of developing neurons and their processes with Anti-DCX (cat. 451-DCX, 1:1000, red) while Anti-MAP2 (cat. 1100-MAP2, 1:10,000, green) labels dendrites and perikarya or mature neurons, and additional nuclear staining was done with DAPI (blue). Anti-doublecortin is an excellent marker of early developing neuronal cells.](http://www.antibodiesinc.com/cdn/shop/files/451-dcx-icc-1_{width}x.jpg?v=1722535123)
![Western blot of postnatal day 3 rat brain lysate showing specific immunolabeling of the ~35 kDa and ~45 kDa doublecortin protein.](http://www.antibodiesinc.com/cdn/shop/files/451-dcx-wb-1_{width}x.jpg?v=1722535124)
![Immunofluorescence of cultured rat neurons showing strong cytoplasmic staining of doublecortin (cat. 451-DCX, 1:1000,green) in developing neurons and GFAP (cat. 621-GFAP, 1:1000, red)](http://www.antibodiesinc.com/cdn/shop/files/451-dcx-icc-2_{width}x.jpg?v=1722535123)
Immunofluorescence of rat cortical neuron-glial cells showing strong cytoplasmic labeling of a small population of developing neurons and their processes with Anti-DCX (cat. 451-DCX, 1:1000, red) while Anti-MAP2 (cat. 1100-MAP2, 1:10,000, green) labels dendrites and perikarya or mature neurons, and additional nuclear staining was done with DAPI (blue). Anti-doublecortin is an excellent marker of early developing neuronal cells.
SKU: 451-DCX
Product Details
Doublecortin
Doublecortin, or DCX, is a microtubule associated protein that is expressed almost exclusively in very early neuronal development (Brown et al., 2003), making it an excellent marker for developing neuronal cells. Defects in the DCX gene lead to X-linked lissencephaly which is characterized by a lack of normal folds on the surface of the brain resulting in a smooth cerebral cortex caused by abnormal migration of neurons during development (des Portes et al., 1998; Gleeson et al., 1998).
Protein G Purified
Monoclonal
3E1
IgG2a
ICC, IHC, WB
Mouse
DCX
35, 45 kDa
Full length recombinant human protein expressed in and purified from E. coli.
Bovine, Human, Mouse, Rat
AB_2492079
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Protein G purified culture supernatant.
PBS + 50% glycerol and 5 mm NaN3
WB: 1:5000
WB Brain: 1:5000
IHC: 1:1000
ICC: 1:1000
WB Brain: 1:5000
IHC: 1:1000
ICC: 1:1000
Unconjugated
Specific for endogenous levels of the ~35 kDa & ~45 kDa doublecortin protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
DBCN antibody, Dbct antibody, DC antibody, DCX antibody, DCX_HUMAN antibody, Doublecortex antibody, Doublin antibody, FLJ51296 antibody, Lis X antibody, Lis-X antibody, Lissencephalin X antibody, Lissencephalin-X antibody, Lissencephaly X linked antibody, Lissencephaly X linked doublecortin antibody, LISX antibody, Neuronal migration protein doublecortin antibody, OTTHUMP00000023859 antibody, OTTHUMP00000023860 antibody, OTTHUMP00000216315 antibody, OTTHUMP00000216316 antibody, SCLH antibody, XLIS antibody
UniProt (Human): O43602
UniProt (Immunogen Species): O43602
UniProt (Immunogen Species): O43602
1641
Product Specific Protocols
- Western Blot Protocol: Download
General References
- Brown, J.P., Couillard‐Després, S., Cooper‐Kuhn, C.M., Winkler, J., Aigner, L. and Kuhn, H.G., 2003. Transient expression of doublecortin during adult neurogenesis. Journal of Comparative Neurology, 467(1), pp.1-10. PMID: 14574675
- des Portes, V., Pinard, J.M., Billuart, P., Vinet, M.C., Koulakoff, A., Carrié, A., Gelot, A., Dupuis, E., Motte, J., Berwald-Netter, Y. and Catala, M., 1998. A novel CNS gene required for neuronal migration and involved in X-linked subcortical laminar heterotopia and lissencephaly syndrome. Cell, 92(1), pp.51-61. PMID: 9489699
- Gleeson, J.G., Allen, K.M., Fox, J.W., Lamperti, E.D., Berkovic, S., Scheffer, I., Cooper, E.C., Dobyns, W.B., Minnerath, S.R., Ross, M.E. and Walsh, C.A., 1998. Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein. Cell, 92(1), pp.63-72.PMID: 9489700