Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (AAT58219.1), rat (NP_445831.3) and human (CAA06617.1) gene products. These antibodies were affinity-purified and then mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.
Doublecortin (1:1000 dilution, green) staining of neuroblastoma cells in culture. Red staining is a Golgi apparatus marker. Page Balisch (University of Arizona).
Doublecortin is a microtubule-associated protein found in neuroblasts and immature neurons, especially those undergoing embryonic migrations. It was first identified as the X-chromosome-encoded gene product mutated in the human diseases Lissencephaly (in males) and Periventricular Heterotopia (in females), diseases caused by failure of neocortical neuroblast migration. The human protein itself is 360 amino acids (40,044 daltons) and contains doublecortin motifs near its N-terminus that are believed to mediate the microtubule binding.
Affinity-Purified IgY
200 µg/mL
Polyclonal
IgY
ICC, IHC
Chicken
DCX DBCN LISX
40 kDa
Synthetic peptide
Human, Mouse, Rat
AB_2313540
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the doublecortin gene product, but are shared between the human (CAA06617.1, NCBI) and mouse (AAT58219.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of these affinity-purified antipeptide antibodies were mixed, and the preparation was filter-sterilized.
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000
This anti-peptide antibody mixture was analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from receipt of product
Ice packs
Neuronal migration protein doublecortin (Doublin) (Lissencephalin-X) (Lis-X)
UniProt (Immunogen Species): O15075
Product Specific References for Applications and Species
Lim, R.G., et al. 2017. Developmental alterations in Huntington''s disease neural cells and pharmacological rescue in cells and mice. Nature Neuroscience, 648-660.
Delepine, C., et al. 2021. GSK3ß inhibitor CHIR 99021 modulates cerebral organoid development through dose-dependent regulation of apoptosis, proliferation, differentiation and migration. Plos One, .
Carpenter, J.M., et al. 2022. Evaluation of Delayed LNFPIII Treatment Initiation Protocol on Improving Long-Term Behavioral and Neuroinflammatory Pathology in a Mouse Model of Gulf War Illness. Brain, Behavior, & Immunity, 100553.
Mellios, N., et al. 2017. MeCP2-regulated miRNAs control early human neurogenesis through differential effects on ERK and AKT signaling. Molecular Psychiatry, 1051-1065.
Smagin, D.A., et al. 2015. Altered Hippocampal Neurogenesis and Amygdalar Neuronal Activity in Adult Mice with Repeated Experience of Aggression. Frontiers in Neurosciene, 443.
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