Anti-Dynamin (Ser774) Antibody

Name: Anti-Dynamin (Ser774) Antibody
Brand: PhosphoSolutions
SKU: p1050-774
Price: 430.0 USD
Availability: InStock

4 Citations

Our Anti-Dynamin (Ser774) sheep polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Dynamin (Ser774) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95 kDa dynamin phosphorylated at Ser774 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Species Reactivity

Mouse, Rat

Applications

ICC, WB

Host Species

Sheep

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Data Sheet

Trial Size

Pooled Serum

SKU: p1050-774

Volume: 100 µL

Variant

Price:

$430.00

Quantity:

Product Details

Target

Dynamin (Ser774)

Target Description

Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser-774 and Ser-778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of Ser-774 by GSK3 has recently been shown to control activity-dependent bulk endocytosis of synaptic vesicles (Clayton et al., 2010).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Sheep

Gene Name

DNM1

Molecular Weight

95 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 of rat dynamin, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate

Antibody Registry ID

AB_2492082

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled sheep serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~95 kDa dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by λ-phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser774

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

B dynamin antibody, D100 antibody, DNM 1 antibody, DNM antibody, DNM1 antibody, DYN1_HUMAN antibody, Dynamin antibody, Dynamin-1 antibody, Dynamin1 antibody

UniProt Details

UniProt (Human): Q05193
UniProt (Immunogen Species): P21575

NCBI Gene ID

140694

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Western Blot: Mouse | Rat

Immunocytochemistry: Rat
PMID	Dilution	Publication
12855954	not listed	Tan, T.C., et al. 2003. Cdk5 is essential for synaptic vesicle endocytosis. Nature Cell Biology, 5(8), p.701.

Western Blot: Mouse
PMID	Dilution	Publication
28052246	not listed	Vargas, K.J., et al. 2017. Synucleins have multiple effects on presynaptic architecture. Cell Reports, 18(1), pp.161-173.

Western Blot: Rat
PMID	Dilution	Publication
18519737	not listed	Leal-Ortiz, S., et al. 2008. Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. The Journal of Cell Biology, 181(5), pp.831-846.
12855954	not listed	Tan, T.C., et al. 2003. Cdk5 is essential for synaptic vesicle endocytosis. Nature Cell Biology, 5(8), p.701.

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Product Specific Protocols

Dynamin Ser774 ICC Protocol: Download

Western Blot Protocol: Download

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Anti-Dynamin (Ser774) Antibody

SKU: p1050-774

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Dynamin (Ser774) Antibody

Product Specific Protocols

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