Anti-Fascin (clone 55K2) Antibody (55K2)

4 Citations

Our Fascin (clone 55K2) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Fascin (clone 55K2) and is Protein A purified. It is great for use in WB, ICC, IHC.

Western blot analysis of human HeLa cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) (lanes 3 & 4).
Immunocytochemical labeling of Fascin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Fascin (FM2651). The antibody was detected using goat anti-mouse DyLight® 594.
Sea urchin embryos were injected with control miR-124 LNA inhibitor or miR-31 LNA inhibitor and immunolabeled with Fascin antibody (cat. FM2651, green) and tubulin antibody (magenta) followed by DAPI counterstain for DNA (blue). Image from publication CC-BY-4.0. PMID: 37398341
Embryos were immunolabeled with Fascin antibodies (cat. FM2651, 1:100) pre-adsorbed with lysate from bacteria expressing HA-tagged Fascin or human Rab35-GST. Embryos immunolabeled with pre-adsorbed Fascin antibodies have decreased Fascin (shown in green) compared to embryos immunolabeled with non-pre-adsorbed Fascin antibody. Image from publication CC-BY-4.0. PMID: 37033709
Western blot analysis of human HeLa cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) (lanes 3 & 4).
Western blot analysis of human HeLa cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) (lanes 3 & 4).
Immunocytochemical labeling of Fascin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Fascin (FM2651). The antibody was detected using goat anti-mouse DyLight® 594.
Sea urchin embryos were injected with control miR-124 LNA inhibitor or miR-31 LNA inhibitor and immunolabeled with Fascin antibody (cat. FM2651, green) and tubulin antibody (magenta) followed by DAPI counterstain for DNA (blue). Image from publication CC-BY-4.0. PMID: 37398341
Embryos were immunolabeled with Fascin antibodies (cat. FM2651, 1:100) pre-adsorbed with lysate from bacteria expressing HA-tagged Fascin or human Rab35-GST. Embryos immunolabeled with pre-adsorbed Fascin antibodies have decreased Fascin (shown in green) compared to embryos immunolabeled with non-pre-adsorbed Fascin antibody. Image from publication CC-BY-4.0. PMID: 37033709

Human, Mouse, Rat
ICC, IHC, WB
Mouse

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SKU: FM2651

Volume: 100 µL
1-2 business days
Price:
$275.00

Product Specific References for Applications and Species

Immunohistochemistry: Sea Urchin
PMID Dilution Publication
37398341not listedRemsburg, C, et al. 2023. miR-31-mediated local translation at the mitotic spindle is important for early development. Research square, .
370337091:100Testa, MD, et al. 2023. The actin bundling protein Fascin is important for proper early development in Strongylocentrotus purpuratus embryos. microPublication Biology, .
Proximity Ligation Assay: Sea Urchin
PMID Dilution Publication
392505311:100Remsburg, CM, et al. 2024. miR-31-mediated local translation at the mitotic spindle is important for early development. Development, .
Western Blot: Mouse
PMID Dilution Publication
28369442not listedElizondo, DM, et al. 2017. CD40-CD40L cross-talk drives fascin expression in dendritic cells for efficient antigen presentation to CD4+ T cells. International immunlogy, 121-131.

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