Anti-Fascin (Ser-39), Phosphospecific Antibody

Name: Anti-Fascin (Ser-39), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: FP2661
Price: 340.0 USD
Availability: InStock

6 Citations

Our Fascin (Ser-39) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Fascin (Ser-39) and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical labeling of fascin phosphorylation relative to F-actin in chick E9 DRG neurons. The cells were labeled with rabbit polyclonal Fascin (Ser-39) antibody, then detected using appropriate secondary antibody (Red). Fascin (Ser-39) labeling is compared (Top) to F-actin staining (Green). (Image provided by Dr. Gianluca Gallo at Drexel University).

Western blot analysis of human HeLa cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) (lanes 3 & 4).

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Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: FP2661

Volume: 100 µL

Variant

Price:

$340.00

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Product Details

Target

Fascin (Ser-39)

Target Description

Fascin is an actin filament bundling protein localized to lamellipodia and filopodia where it has important roles in cell motility. Regulation of fascin occurs through PKC-mediated phosphorylation of Ser-39 in the F-actin binding site. Cell permeant peptides that block PKC phosphorylation of Ser-39 increase cell migration, while peptides that block fascin binding to F-actin alter lamellipodial morphology and cause aberrant cell motility. Studies using RNA interference of fascin show that fibroblasts have reduced number and abnormal morphology of filopodia, while Ser-39 phosphorylation status may determine filopodial frequency. In Drosophila neurons, fascin deficiency causes alterations in actin filaments and leads to abnormal morphology of developing neurons. Thus, fascin is a critical element of actin-based motility in various cell types.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

FSCN1

Molecular Weight

Antigen

Fascin (Ser-39) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 39 in human fascin. This sequence has high homology to the conserved site in rat and mouse fascin, as well as to the conserved region in fascin-2, but has low homology to the conserved region in fascin-3.

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to a phospho-serine peptide before affinity purified using phosho-fascin (Ser-39) peptide (without carrier). The antibody detects a 55 kDa* band corresponding to fascin on SDS-PAGE immunoblots of mouse C2C12 and human HeLa cells treated with Calyculin A. This band is not observed after lambda phosphatase treatment. The antibody also detects fascin (Ser-39) phosphorylation in chick DRG neurons.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-39

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

p55

UniProt ID

Q16658

Product Specific References for Applications and Species

Co-Immunoprecipitation: Human
Immunocytochemistry: Human
Immunohistochemistry: Human
Western Blot: Human | Mouse

Co-Immunoprecipitation: Human
PMID	Dilution	Publication
27819326	not listed	Saad, A., et al. 2016. Insights into a novel nuclear function for Fascin in the regulation of the amino-acid transporter SLC3A2. Scientific Reports, 36699.

Immunocytochemistry: Human
PMID	Dilution	Publication
27819326	1:1000	Saad, A., et al. 2016. Insights into a novel nuclear function for Fascin in the regulation of the amino-acid transporter SLC3A2. Scientific Reports, 36699.

Immunohistochemistry: Human
PMID	Dilution	Publication
27819326	1:50	Saad, A., et al. 2016. Insights into a novel nuclear function for Fascin in the regulation of the amino-acid transporter SLC3A2. Scientific Reports, 36699.

Western Blot: Human
PMID	Dilution	Publication
29088820	1:50	Rodrigues, P.C., et al. 2017. Fascin promotes migration and invasion and is a prognostic marker for oral squamous cell carcinoma. Oncotarget, 74736-74754.
27819326	1:1000	Saad, A., et al. 2016. Insights into a novel nuclear function for Fascin in the regulation of the amino-acid transporter SLC3A2. Scientific Reports, 36699.
24414419	not listed	Van Audenhove, I., et al. 2014. Stratifying fascin and cortactin function in invadopodium formation using inhibitory nanobodies and targeted subcellular delocalization. FASEB Journal, 1805-1818.

Western Blot: Mouse
PMID	Dilution	Publication
30683702	not listed	Bulek, K., et al. 2019. IL-17A Recruits Rab35 to IL-17R to Mediate PKCα-Dependent Stress Fiber Formation and Airway Smooth Muscle Contractility. Journal of Immunology, 1540-1548.
28855604	not listed	Kliewe, F, et al. 2017. Studying the role of fascin-1 in mechanically stressed podocytes. Scientific reports, 9916.

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Anti-Fascin (Ser-39), Phosphospecific Antibody

SKU: FP2661

Product Details

Product Specific References for Applications and Species

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