Anti-FHOD1 (Thr-1141), Phosphospecific Antibody

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Our FHOD1 (Thr-1141) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat FHOD1 (Thr-1141) and is antigen affinity purified. It is great for use in WB.

Western blot of FHOD1 phosphorylation in human K562 cells stimulated with calyculin A (100 nM) for 30 min. (lanes 1 & 3). The blot was then treated with lambda phosphatase (lanes 2 & 4). Blots were probed with mouse polyclonal anti-FHOD1 (lanes 1 & 2) and anti-FHOD1 (Thr-1141), phospho-specific antibody (lanes 3 & 4) .
Western blot of FHOD1 phosphorylation in human K562 cells stimulated with calyculin A (100 nM) for 30 min. (lanes 1 & 3). The blot was then treated with lambda phosphatase (lanes 2 & 4). Blots were probed with mouse polyclonal anti-FHOD1 (lanes 1 & 2) and anti-FHOD1 (Thr-1141), phospho-specific antibody (lanes 3 & 4) .
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Western blot of FHOD1 phosphorylation in human K562 cells stimulated with calyculin A (100 nM) for 30 min. (lanes 1 & 3). The blot was then treated with lambda phosphatase (lanes 2 & 4). Blots were probed with mouse polyclonal anti-FHOD1 (lanes 1 & 2) and anti-FHOD1 (Thr-1141), phospho-specific antibody (lanes 3 & 4) .

Human, Mouse, Rat
WB
Rabbit

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Data Sheet

SKU: FP3481

Volume: 100 µL
Price:
$340.00
Ships: 1-2 business days

Product Details

FHOD1 (Thr-1141)
Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. The FH1 region contains poly-proline stretches that promote interactions with profilin. The FH2 domain, located C-terminally to the FH1 domain, is highly conserved in formin proteins and possesses actin nucleation and polymerization activities. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. Several mammalian formins, including mDia1, FRL, and formin homology domain protein 1 (FHOD1) are inhibited through an intramolecular interaction between the C-terminal Dia autoregulatory domain (DAD) and its recognition region at the N-terminus. In FHOD1, this autoinhibitory interaction is disrupted through phosphorylation of Ser-1131, Ser-1137, and Thr-1141 by ROCK. Subsequent FHOD1 activation leads to stress fiber formation. In endothelial cells, thrombin activates this ROCK pathway, leading to FHOD1-mediated stress fiber formation.
Antigen Affinity Purified
Polyclonal
IgG
WB
Rabbit
FHOD1
140
Phospho-FHOD1 (Thr-1141) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Thr-1141 in human FHOD1. This peptide sequence is highly conserved in rat and mouse FHOD1, and is well conserved in FHOD3 (Thr-1399).
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
Unconjugated
This antibody was cross-adsorbed to unphosphorylated FHOD1 (Thr-1141) peptide before affinity purification using phospho-FHOD1 (Thr-1141) peptide (without carrier). The antibody detects a 140 kDa* protein corresponding to the apparent molecular mass of FHOD1 on SDS-PAGE immunoblots of human K562 and mouse C2C12 cells treated with calyculin A.
Phosphorylated
Thr-1141
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
FHOS, formin
Q9Y613

Product Specific References for Applications and Species

Western Blot: Human
PMID Dilution Publication
29885024not listedBoddy, K.C., et al. 2018. Septin-regulated actin dynamics promote Salmonella invasion of host cells. Cell Microbiology, e12866.
23869992not listedTruong, D., et al. 2013. Formin-mediated actin polymerization promotes Salmonella invasion. Cell Microbiology, 2051-2063.
Western Blot: Mouse
PMID Dilution Publication
29396114not listedPandey, P, et al. 2018. Cardiomyocytes Sense Matrix Rigidity through a Combination of Muscle and Non-muscle Myosin Contractions. Developmental cell, 326-336.
25125170not listedDwyer, J., et al. 2014. The formin FHOD1 in cardiomyocytes. Anatomical Recort, 1560-1570.
24331927not listedIskratsch, T., et al. 2013. FHOD1 is needed for directed forces and adhesion maturation during cell spreading and migration. Developmental cell, 545-559.
21106951not listedStaus, D.P., et al. 2011. Formin homology domain-containing protein 1 regulates smooth muscle cell phenotype. Arterioschlerosis, Thrombosis, and Vascular Biology, 360-367.

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