Anti-γ-Catenin (C-terminal region) Antibody
Our γ-Catenin (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat γ-Catenin (C-terminal region) and is antigen affinity purified. It is great for use in WB.
SKU: CP2971
Product Details
γ-Catenin (C-terminal region)
Plakoglobin (γ-Catenin) is a catenin family member identified as a component of desmosomes. γ-Catenin has high homology to β-catenin and, like β-catenin, it can associate with the cadherins, E-cadherin and N-cadherin. One molecule of α-catenin and at least one molecule of β-catenin and γ-Catenin simultaneously bind to a single cadherin molecule. A 19-amino acid sequence of desmoglein was found to be critical for binding of γ-Catenin. Similar catenin-binding domains found in cadherins, suggest a common mechanism for γ-Catenin localization to both adherens junctions and desmosomes. Phosphorylation of tyrosine residues in γ-Catenin can modify its interactions with other proteins. Phosphorylation of tyrosine 644 decreases γ-Catenin association with α-catenin, but increases binding to desmoplakin. Fer kinase can phosphorylate tyrosine 550, which increases γ-Catenin binding to α-catenin. Thus, tyrosine phosphorylation may be important for regulation of γ-Catenin protein-protein interactions within desmosomal complexes.
Antigen Affinity Purified
Polyclonal
IgG
WB
Rabbit
JUP
84
γ-Catenin synthetic peptide (coupled to KLH) corresponding to amino acid residues from the C-terminal region of human γ-Catenin. This peptide sequence is highly conserved in rat and mouse γ-Catenin.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
Unconjugated
The antibody detects an 84kDa* protein corresponding to the molecular mass of γ-Catenin on SDS-PAGE immunoblots of A431 cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Plakoglobin, JUP, Desmoplakin III, Desmoplakin-3, catenin gamma1
UniProt (Human): P14923
UniProt (Immunogen Species): P14923
UniProt (Immunogen Species): P14923
Product Specific References for Applications and Species
- Western Blot: Human
Western Blot: Human | ||
PMID | Dilution | Publication |
23110151 | not listed | Cooksley-Decasper, S., et al. 2012. Antibody phage display assisted identification of junction plakoglobin as a potential biomarker for atherosclerosis. PLoS One, e47985. |