Our Anti-GABA-A-R-Gamma2L mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N452/69. It detects human, mouse, and rat GABA-A-R-Gamma2L, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Gamma-aminobutyric acid receptor subunit gamma-2, or GABA AR gamma2 is encoded by the gene GABRG2. GABA A receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR gamma2 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR gamma2 has been found expressed in stomach and bladder. In brain, GABA AR gamma2 is expressed at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane. Mutations in this gene have been associated with epilepsy and febrile seizures. Note that GABA AR γ2 subunit has two forms, designated “short” (γ2S) and “long” (γ2L) that differ only in eight amino acids inserted into the large intracellular loop of the subunit (Whiting et al., 1990).
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N452/69
IgG2a
ICC, IHC, IP, WB
Mouse
GABRG2
45 kDa
Fusion protein amino acids 360-445 (cytoplasmic C-terminus) of human GABA-A-R-Gamma2L (accession number P18507-2) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2617122
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:1000
ICC: 1:1000
IP: 4 ug
Unconjugated
Does not cross-react with GABA-A-R-Gamma2S, GABA-A-R-Gamma1 or GABAA-A-R-Gamma3
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Chen, M., et al. 2023. Blue Native PAGE-Antibody Shift in Conjunction with Mass Spectrometry to Reveal Protein Subcomplexes: Detection of a Cerebellar α1/α6-Subunits Containing γ-Aminobutyric Acid Type A Receptor Subtype. International Journal of Molecular Sciences, 7632.
Nathanson, A.J., et al. 2019. Identification of a core amino acid motif within the α subunit of GABAARs that promotes inhibitory synaptogenesis and resilience to seizures. Cell Reports, 670-681.
Rajgor, D., et al. 2020. Local miRNA-dependent translational control of GABAAR synthesis during inhibitory long-term potentiation. Cell reports, 31(12), p.107785.. Cell reports, 107785.
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