Anti-GABAA Receptor α2 Antibody
Our Anti-GABAA Receptor α2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse GABAA Receptor α2 and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.
Mouse, Rat
ICC, WB
Rabbit
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SKU: 822-GA2CL
Product Details
GABAA Receptor α2
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channelassociated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunitsresults in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ICC, WB
Rabbit
GABRA2
51 kDa
Fusion protein from the cytoplasmic loop of the α2 subunit of rat GABAA receptor.
Mouse, Rat
Bovine, Canine, Human, Non-Human Primate, Rat, Zebrafish
AB_2492101
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
ICC: 1:1000
WB Brain: 1:1000
ICC: 1:1000
Unconjugated
Specific for endogenous levels of the ~51 kDa α2-subunit of the GABAA receptor in Western blots. Labeling is absent in α2-subunit knockout animals.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
GABA A receptor subunit alphα2 antibody, GABA antibody, GABA(A) receptor subunit alphα2 antibody, GABA(A) receptor subunit alpha-2 antibody, GABR A2 antibody, GABRα2 antibody, GABRA2 antibody, GABRA2 protein antibody, Gamma aminobutyric acid (GABA) A receptor alphα2 antibody, Gamma aminobutyric acid A receptor alphα2 antibody, Gamma aminobutyric acid receptor alphα2 subunit antibody, Gamma aminobutyric acid receptor subunit alphα2 antibody, Gamma-aminobutyric acid receptor subunit alpha-2 antibody, GBRA2_HUMAN antibody
UniProt (Human): P47869
UniProt (Immunogen Species): P23576
UniProt (Immunogen Species): P23576
61856
Product Specific References for Applications and Species
- Western Blot: Rat
Western Blot: Rat | ||
PMID | Dilution | Publication |
36323250 | not listed | Chan, E.S., et al. 2022. Allosteric potentiation of GABAA Receptor Single-Channel Conductance by Netrin-1 During Neuronal-Excitation-Induced Inhibitory Synaptic Homeostasis. Cell Reports, 111584. |
Product Specific Protocols
- Western Blot Protocol: Download