Anti-GABA-B-R1 Antibody (N93A/49)
Our Anti-GABA(B)R1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N93A/49. It is KO validated, detects human and mouse GABA(B)R1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Immunoblot versus crude membranes made from adult rat brain (RBM) or GABABR1 wild-type (WT) or knockout (KO) mice probed with N93A/49 (left) or K89/34 (right) TC supe. Mouse brains courtesy of Martin Gassmann and Bernhard Bettler (University of Basel).
Chicken, Human, Mouse, Rabbit, Rat
ICC, IHC, WB
Mouse
KO Validated
Product Specific References for Applications and Species
- dSTORM: Mouse
- Immunocytochemistry: Mouse | Rat
- Immunohistochemistry: Chicken | Mouse | Rabbit | Rat
- Immuno Gold Pre/Post Embedd: Mouse
- PLA: Mouse | Rat
- Western Blot: Human | Mouse | Rat
- Additional Publications: Unspecified
| dSTORM: Mouse | ||
| PMID | Dilution | Publication |
| 33888718 | 1:1000 | Fujun, L., et al. 2021. Neurexins regulate presynaptic GABAB-receptors at central synapses. Nature Communications, 2380. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 36395772 | not listed | Kleinjan, M.S., et al. 2023. Dually Innervated Dendritic Spines Develop In The Absence Of Excitatory Activity And Resist Plasticity Through Tonic Inhibitory Crosstalk. Neuron, 362-371. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 28660246 | 1:50 | Li, P., et al. 2017. GABA-B controls persistent Na+ current and coupled Na+-activated K+ current. eNeuro, . |
| 26212383 | 1 : 15 | Karls, A., et al. 2015. GABA(B) receptors couple to Gαq to mediate increases in voltage-dependent calcium current during development.. Journal of Neurochemistry, 88-100. |
| Immunohistochemistry: Chicken | ||
| PMID | Dilution | Publication |
| 26298628 | 1:8 (supe) | Frederikse, P.H., et al. 2015. Fragile X Syndrome FMRP Co-localizes with Regulatory Targets PSD-95, GABA Receptors, CaMKIIα, and mGluR5 at Fiber Cell Membranes in the Eye Lens.. Neurochemical Research, 2167-2176. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 39358985 | not listed | Mitten, EH, et al. 2024. Chronic ethanol exposure in mice evokes pre- and postsynaptic deficits in GABAergic transmission in ventral tegmental area GABA neurons. British Journal of Pharmacology, . |
| 39353038 | not listed | Luo, H, et al. 2024. Amyloid-β oligomers trigger sex-dependent inhibition of GIRK channel activity in hippocampal neurons in mice. Science Signaling, eado4132. |
| 38177340 | 1:400 | Haruwaka, K, et al. 2024. Microglia enhance post-anesthesia neuronal activity by shielding inhibitory synapses. Nature Neuroscience, . |
| 33888718 | 1:500 | Fujun, L., et al. 2021. Neurexins regulate presynaptic GABAB-receptors at central synapses. Nature Communications, 2380. |
| 31915042 | 1:500 | Rice, H.C., et al. 2020. Contribution of GABAergic interneurons to amyloid-β plaque pathology in an APP knock-in mouse model. Molecular Neurodengeneration, 3. |
| 21336828 | 1:100 | Broussard, D.M., et al. 2011. Motor learning in the VOR: the cerebellar component.. Experimental Brain Research, 451-463. |
| Immunohistochemistry: rabbit | ||
| PMID | Dilution | Publication |
| 26539026 | not listed | Frederikse, P.H., et al. 2015. KCC2 expression supersedes NKCC1 in mature fiber cells in mouse and rabbit lenses.. Molecular Vision, 1142-1150. |
| 26298628 | 1:8 (supe) | Frederikse, P.H., et al. 2015. Fragile X Syndrome FMRP Co-localizes with Regulatory Targets PSD-95, GABA Receptors, CaMKIIα, and mGluR5 at Fiber Cell Membranes in the Eye Lens.. Neurochemical Research, 2167-2176. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 23097697 | 1:500 | Kramer, A.A., et al. 2012. Levels of Ca(V)1.2 L-Type Ca(2+) Channels Peak in the First Two Weeks in Rat Hippocampus Whereas Ca(V)1.3 Channels Steadily Increase through Development.. Journal of Signal Transduction, 597214. |
| Immuno Gold Pre/Post Embedd: Mouse | ||
| PMID | Dilution | Publication |
| 34248508 | 10ug/ml | Roldán-Sastre, A., et al. 2021. Cellular Diversity and Differential Subcellular Localization of the G-Protein Gαo Subunit in the Mouse Cerebellum. Frontiers of Neuroanatomy, 686279. |
| 26808347 | 1-2ug/ml | Lecca, S., et al. 2016. Rescue of GABAB and GIRK function in the lateral habenula by protein phosphatase 2A inhibition ameliorates depression-like phenotypes in mice. Nature Medicine, 254-261. |
| 22405207 | not listed | Padgett, C.L., et al. 2012. Methamphetamine-evoked depression of GABA(B) receptor signaling in GABA neurons of the VTA.. Neuron, 978-989. |
| PLA: Mouse | ||
| PMID | Dilution | Publication |
| 27573246 | 1:100 | Zemoura, K., et al. 2016. Lys-63-linked ubiquitination of γ-aminobutyric acid (GABA), type B1, at multiple sites by the E3 ligase mind bomb-2 targets GABAB receptors to lysosomal …. Journal of Biological Chemistry, 21682-21693. |
| PLA: Rat | ||
| PMID | Dilution | Publication |
| 32111696 | not listed | Burke, J.F., et al. 2020. Direct interaction of PP2A phosphatase with GABAB receptors alters functional signaling. Journal of Neuroscience, 2808-2816. |
| 29881949 | 1:50 | Zemoura, K., et al. 2019. Ca2+/Calmodulin-Dependent Protein Kinase II (CaMKII) β-Dependent Phosphorylation of GABAB1 Triggers Lysosomal Degradation of GABAB Receptors via Mind Bomb-2 (MIB2)-Mediated Lys-63-Linked Ubiquitination. Molecular Neurobiology, 1293-1309. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 39028675 | 1:1000 | Minere, M., et al. 2024. Presynaptic hyperexcitability reversed by positive allosteric modulation of a GABABR epilepsy variant. Brain, . |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 25172509 | not listed | Huyghe, D., et al. 2014. Glutamine synthetase stability and subcellular distribution in astrocytes are regulated by γ-aminobutyric type B receptors. . Journal of Biological Chemistry, 28808-28815. |
| 24431439 | not listed | Terunuma, M., et al. 2014. Postsynaptic GABAB receptor activity regulates excitatory neuronal architecture and spatial memory.. Journal of Neuroscience, 804-816. |
| 22207761 | 1:200 | Schwindinger, W.F., et al. 2012. Synergistic roles for G-protein γ3 and γ7 subtypes in seizure susceptibility as revealed in double knock-out mice.. The Journal of Biological Chemistry, 7121-7133. |
| 21636656 | 1:250 | Pacey, L.K., et al. 2011. Subchronic administration and combination metabotropic glutamate and GABAB receptor drug therapy in fragile X syndrome.. Journal of Pharmacology and Experimental Therapeutics, 897-905. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 28450542 | not listed | Wright, R., et al. 2017. Neuronal chloride regulation via KCC2 is modulated through a GABAB receptor protein complex. . Journal of Neuroscience, 5447-5462. |
| 26691831 | not listed | Schwenk, J., et al. 2016. Modular composition and dynamics of native GABAB receptors identified by high-resolution proteomics.. Nature Neuroscience, 233-242. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 21215802 | Pacey, L.K., et al. 2011. Genetic deletion of regulator of G-protein signaling 4 (RGS4) rescues a subset of fragile X related phenotypes in the FMR1 knockout mouse.. Molecular and Cellular Neuroscience, 563-572. | |