Anti-GABAA Receptor, ß3 (Ser408/409) Antibody

Name: Anti-GABAA Receptor, ß3 (Ser408/409) Antibody
Brand: PhosphoSolutions
SKU: p1130-4089
Price: 430.0 USD
Availability: InStock

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Our Anti-GABAA Receptor, ß3 (Ser408/409) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GABAA Receptor, ß3 (Ser408/409) and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~58 kDa GABAA β3 protein phosphorylated at Ser408/409 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Species Reactivity

Mouse, Rat

Applications

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: p1130-4089

Volume: 100 µL

Variant

Price:

$430.00

Quantity:

Product Details

Target

GABAA Receptor, ß3 (Ser408/409)

Target Description

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABA-A and the GABA-B subtype of receptors. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. Phosphorylation of serine 408 and serine 409 within the β3 subunit have been shown to be critical for the functional modulation of β3 containing recombinant receptors (Brandon et al., 2000).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

GABRB3

Molecular Weight

53 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser408/409 of the ß3 subunit of rat GABAA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus, Zebrafish

Antibody Registry ID

AB_2492111

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~53 kDa GABAA receptor 3 subunit phosphorylated at Ser408/409. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser408,409

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

ECA5 antibody, GABA alpha receptor beta-2 subunit antibody, GABA(A) receptor subunit beta-3 antibody, GABAA receptor beta 3 subunit antibody, GABAA receptor subunit beta 3 antibody, GABR B3 antibody, Gabrb3 antibody, Gamma aminobutyric acid (GABA) A receptor beta 3 antibody, Gamma aminobutyric acid receptor subunit beta 3 antibody, Gamma-aminobutyric acid receptor subunit beta-3 antibody, GBRB3_HUMAN antibody, MGC9051 antibody

UniProt Details

UniProt (Human): P28472
UniProt (Immunogen Species): P63079

NCBI Gene ID

24922

Product Specific References for Applications and Species

Western Blot: Mouse | Rat

Western Blot: Mouse
PMID	Dilution	Publication
39264811	1:1000	Ma, S., et al. 2024. Deletion of histamine H2 receptor in VTA dopaminergic neurons of mice induces behavior reminiscent of mania. Cell Reports, 114717.
30077648	not listed	Brewer,A.L., et al. 2018. Role of spinal GABA receptors in the acute antinociceptive response of mice to hyperbaric oxygen. Brain research, 1699, pp.107-116.

Western Blot: Rat
PMID	Dilution	Publication
36787750	1:500	Rosenberg, E.C., et al. 2023. Cannabidiol Modulates Excitatory-Inhibitory Ratio to Counter Hippocampal Hyperactivity. Neuron, .
36233065	1:500	Arenas, Y.M., et al. 2022. Enhanced BDNF and TrkB Activation Enhance GABA Neurotransmission in Cerebellum in Hyperammonemia. International Journal of Molecular Sciences, .
25274828	not listed	Riffault, B., et al. 2014. Pro-Brain-Derived Neurotrophic Factor Inhibits GABAergic Neurotransmission by Activating Endocytosis and Repression of GABA_A Receptors. The Journal of Neuroscience, 34(40), 13516-13534.

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Product Specific Protocols

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Anti-GABAA Receptor, ß3 (Ser408/409) Antibody

SKU: p1130-4089

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-GABAA Receptor, ß3 (Ser408/409) Antibody

Product Specific Protocols

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