Our Anti-GFAP mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N206A/8. It is KO validated, detects human, mouse, and rat GFAP, and is purified by Protein A chromatography. It is great for use in IHC, ICC.
Drosophila, Guinea Pig, Human, Mouse, Non-Human Primate, Polar Bear, Rat
Glial fibrillary acidic protein (GFAP) is an intermediate filament with many different isoforms, and acts as a structural protein in the cytoskeleton. It is expressed in astrocytes in brain and is often used as an astrocyte marker. GFAP is belived to be involved in many CNS processes including cell communication and mitosis. GFAP has been found to be involved in Alexander disease and Wernicke's encephalopathy.
Purified by Protein A chromatography
0.5 mg/mL
Monoclonal
N206A/8
IgG1
ICC, IHC
Mouse
GFAP
50 kDa
Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP (accession number P14136)
Drosophila, Guinea Pig, Human, Mouse, Non-Human Primate, Polar Bear, Rat
AB_2939875
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
PBS with 0.09% azide
FL650 Ex: 655 nm, Em: 676 nm
Cross-reacts with GFAP-R416W and other GFAP mutant proteinsDoes not cross-react with other proteins (based on KO validation results)
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
KO Validated