Anti-GFAP-R416WT Antibody (N206B/9)

Name: Anti-GFAP-R416WT Antibody
Brand: NeuroMab
SKU: 75-279
Price: 319.0 USD
Availability: InStock

Our Anti-GFAP-R416WT mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N206B/9. It is KO validated, detects human, mouse, and rat GFAP-R416WT, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

KC rabbit anti- Kv2.1 Brain samples courtesy of Albee Messing, UW- Madison.

Immunofluorescence of GFAP WT and KO mouse hippocampus with N206B/9 Brain samples courtesy of Albee Messing, UW- Madison.

Immunoblot versus brain samples from adult rat (RBLSP) and GFAP wild- type (WT), knockout (KO) and R416W KI mice. Mutant control courtesy of Michael Brenner, UAB.

KC rabbit anti- Kv2.1 Brain samples courtesy of Albee Messing, UW- Madison.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-279

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

GFAP-R416WT

Target Description

Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers .

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N206B/9

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

GFAP

Molecular Weight

50 kDa

Antigen

Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP (accession number P14136)

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_11000169

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with GFAP-R416W or other proteins (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Glial fibrillary acidic protein (GFAP)

UniProt Details

UniProt (Human): P14136
UniProt (Immunogen Species): P14136

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Mouse | Rat

Immunocytochemistry: Human
PMID	Dilution	Publication
39103318	1:200	Han, X., et al. 2024. Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex. Nature Communications, 6648.
35625761	1:500	De Kleijn, K.M.A., et al. 2022. Molecular Signature of Neuroinflammation Induced in Cytokine-Stimulated Human Cortical Spheroids. Biomedicines, 1025.
30680309	1:1000	Chander, P., et al. 2019. Neuron-Specific Gene 2 (NSG2) Encodes an AMPA Receptor Interacting Protein That Modulates Excitatory Neurotransmission. eNeuro, .

Immunohistochemistry: Human
PMID	Dilution	Publication
29345029	1:1000	Lütt, A., et al. 2018. High prevalence and functional effects of serum antineuronal antibodies in patients with gastrointestinal disorders. Neurogastroenterology and Motility, e13292.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
31207335	1:500	Olde Heuvel, F., et al. 2019. STAT6 mediates the effect of ethanol on neuroinflammatory response in TBI. Brain, Behavior, and Immunity, 228-246.

Immunohistochemistry: Rat
PMID	Dilution	Publication
39103318	1:200	Han, X., et al. 2024. Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex. Nature Communications, 6648.
32730865	1:1000	Stanisavljević, A., et al. 2020. Fluoxetine modulates neuronal activity in stress-related limbic areas of adult rats subjected to the chronic social isolation. Brain Research Bulletin, 95-108.

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Anti-GFAP-R416WT Antibody (N206B/9)

SKU: 75-279

Product Details

Product Specific References for Applications and Species

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