Anti-GFP (Green Fluorescent Protein) Antibody (N86/44)

Name: Anti-GFP (Green Fluorescent Protein) Antibody
Brand: NeuroMab
SKU: 75-414
Price: 319.0 USD
Availability: InStock

1 Citation

Our Anti-GFP (green fluorescent protein) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N86/44. It detects GFP (green fluorescent protein), and is purified by Protein A chromatography. It is great for use in ICC, WB.

Applications

ICC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-414

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

GFP (green fluorescent protein)

Target Description

Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model organisms.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N86/44

Isotype

IgG2b

Applications

ICC, WB

Host Species

Mouse

Gene Name

GFP

Molecular Weight

30 kDa

Antigen

Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

Antibody Registry ID

AB_2532048

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively)Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively)

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Green fluorescent protein

UniProt Details

UniProt (Human): N/A
UniProt (Immunogen Species): P42212

Product Specific References for Applications and Species

Western Blot: Rat

Western Blot: Rat
PMID	Dilution	Publication
28771710	1:1000	Lee, M.L., et al. 2017. Brain endothelial cells induce astrocytic expression of the glutamate transporter GLT-1 by a Notch-dependent mechanism. Journal of Neurochemistry, 489-506.

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Anti-GFP (Green Fluorescent Protein) Antibody (N86/44)

SKU: 75-414

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-GFP (Green Fluorescent Protein) Antibody

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