Anti-GIT1 Antibody (N39B/8)

11 Citations

Our Anti-GIT1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N39B/8. It is KO validated, detects human, mouse, and rat GIT1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Sliced human breast tumor was stained using Anti-GIT1 antibody (NeuroMab, Cat. No. 75-094, Clone N39/8, 1000x), then followed by anti-Mouse IgG (H+L) secondary antibody, Alexa FluorTM (Invitrogen, A-21425) and DAPI for nuclei. Scale bar 10 μm. Image kindly provided by Songbai Zhang, Karolinska Institutet, Stockholm, Sweden.
Sliced human breast tumor was stained using Anti-GIT1 antibody (NeuroMab, Cat. No. 75-094, Clone N39/8, 1000x), then followed by anti-Mouse IgG (H+L) secondary antibody, Alexa FluorTM (Invitrogen, A-21425) and DAPI for nuclei. Scale bar 10 μm. Image kindly provided by Songbai Zhang, Karolinska Institutet, Stockholm, Sweden.
Roll over image to zoom in Click on image to zoom
Sliced human breast tumor was stained using Anti-GIT1 antibody (NeuroMab, Cat. No. 75-094, Clone N39/8, 1000x), then followed by anti-Mouse IgG (H+L) secondary antibody, Alexa FluorTM (Invitrogen, A-21425) and DAPI for nuclei. Scale bar 10 μm. Image kindly provided by Songbai Zhang, Karolinska Institutet, Stockholm, Sweden.
Adult rat hippocampus immunohistochemistry.
Adult rat brain membrane immunoblot.
Immunoblot versus lysates of HEK293T cells untransfected and transfected with Flag-tagged GIT1 and GIT2 plasmids and probed with N39B/8 (top) and anti-Flag (bottom) antibodies. Data courtesy of Eun-Kyoung Hahm, Jaewon Ko, and Eunjoon Kim, Korea Advanced Institute of Science and Technology.
Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].

Human, Mouse, Rat
ICC, IHC, IP, WB
Mouse
490
550
594
650
Carrier-Free

Go to Full Product Details

Data Sheet
KO Validated KO Validated

SKU: 75-094

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

GIT1
G-protein coupled receptor kinase interacting ArfGAP 1 is encoded by the gene GIT1. GIT1 is a member of the GIT protein family. GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. GIT1 is expressed in numerous tissues with the highest levels in the testis. Diseases associated with GIT1 include Attention Deficit-Hyperactivity Disorder.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N39B/8
IgG1
ICC, IHC, IP, WB
Mouse
Git1
90 kDa
Fusion protein amino acids 375-770 (C-terminus) of rat GIT1(accession number Q9Z272) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2109991
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.21
Unconjugated
No cross-reactivity against GIT2
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
ARF GTPase-activating protein GIT1 (ARF GAP GIT1) (Cool-associated and tyrosine-phosphorylated protein 1) (CAT-1) (CAT1) (G protein-coupled receptor kinase-interactor 1) (GRK-interacting protein 1)
Q9Z272

Product Specific References for Applications and Species

Immunohistochemistry: Human
PMID Dilution Publication
353183021:1000Zhang, S., et al. 2022. GIT1 protects against breast cancer growth through negative regulation of Notch. Nature communications, 1537.
Immunoprecipitation: Human
PMID Dilution Publication
353183026ugZhang, S., et al. 2022. GIT1 protects against breast cancer growth through negative regulation of Notch. Nature communications, 1537.
Western Blot: Human
PMID Dilution Publication
353183021:4000Zhang, S., et al. 2022. GIT1 protects against breast cancer growth through negative regulation of Notch. Nature communications, 1537.
23784547not listedAslan, J.E., et al. 2013. The PAK system links Rho GTPase signaling to thrombin-mediated platelet activation. American Journal of Physiology. Cell Physiology, C519-C528.
21900245not listedWade, R., et al. 2011. Paxillin enables attachment-independent tyrosine phosphorylation of focal adhesion kinase and transformation by RAS.. The Journal of Biological Chemistry, 37932-37944.
Western Blot: Mouse
PMID Dilution Publication
307845871:500Davenport, E., et al. 2019. Autism and Schizophrenia-Associated CYFIP1 Regulates the Balance of Synaptic Excitation and Inhibition. Cell Reports, 2037-2051.
28706196not listedWilkinson, B., et al. 2017. Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling. Scientific Reports, 5272.
24942580not listedBrimer, N., et al. 2014. Interactions between E6, FAK, and GIT1 at paxillin LD4 are necessary for transformation by bovine papillomavirus 1 E6.. Journal of Virology, 9927-9933.
20660256not listedMoreau, M.M., et al. 2010. The planar polarity protein Scribble1 is essential for neuronal plasticity and brain function.. Journal of Neuroscience, 9738-9752.
Western Blot: Rat
PMID Dilution Publication
242979291:500Fiuza, M., et al. 2013. GluN3A expression restricts spine maturation via inhibition of GIT1/Rac1 signaling. PNAS USA, 20807-20812.
Additional Publications: Unspecified
PMID Publication
21499268Won, H., et al. 2011. GIT1 is associated with ADHD in humans and ADHD-like behaviors in mice.. Nature Medicine, 566-572.
19303398Morimura, S., et al. 2009. BetaPIX and GIT1 regulate HGF-induced lamellipodia formation and WAVE2 transport.. Biochemical and Biophysical Research Communications, 614-619.
19022416Klemmer, P., et al. 2009. Proteomics analysis of immuno-precipitated synaptic protein complexes.. Journal of Proteomics, 82-90.

Bulk Order Anti-GIT1 Antibody

Related Products

Recently Viewed