Our Anti-GOLGB1 (Giantin) chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human GOLGB1 (Giantin), and is affinity-purified IgY. It is great for use in IHC and ICC.
Immunofluorescent staining of HeLa cells using 2 µg/mL Aves Labs chicken anti-Giantin (GOLGB1) (Cat. No. GOLGB1-0100) antibody (green). Actin filaments were stained using Phalloidin (red). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. No. AR-6505). Anti-Giantin specifically stains the Golgi apparatus in HeLa cells.
GOLGB1, also known as Giantin, is a protein associated with the Golgi apparatus, playing a key role in maintaining its structural integrity and facilitating vesicle trafficking between the endoplasmic reticulum and the Golgi. Discovered within the context of Golgi apparatus research initiated by Camillo Golgi in 1898, the molecular cloning and sequencing of the GOLGB1 gene in the early 1990s allowed detailed studies of its structure and function, revealing its role in stacking Golgi cisternae and influencing various cell signaling pathways. Functional studies, including knockdown and knockout experiments (Satoh et al 2019), have demonstrated GOLGB1’s importance in maintaining Golgi integrity. A myriad of diseases are associated with Golgi dysfunction, including neurodegenerative diseases such as ALS, Alzheimer’s, and Parkinson’s, as well as cancer and congenital disorders of glycosylation (Lan etc al, 2016). In time, GOLGB1 (Giantin) could potentially serve as a therapeutic target for many of these conditions.
Affinity-Purified IgY
200 µg/mL
Polyclonal
IgY
ICC, IHC
Chicken
GOLGB1
376 kDa
Synthetic peptide
Human
AB_3105810
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
IHC: 1:100-1:500
ICC: 1:100-1:500
Unconjugated
No cross-reactivity reported
Each new lot of this antibody is tested to confirm that it gives specific staining by immunocytochemistry of HeLa cells.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from receipt of product
Ice packs
Golgin B1, GCP372, GOLIM1, GCP, Macrogolgin, Golgi Integral Membrane Protein 1, Golgin Subfamily B Member 1,
UniProt (Human): Q14789
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