Anti-Green Fluorescent Protein Antibody

Name: Anti-Green Fluorescent Protein Antibody
Brand: Aves Labs
SKU: GFP-1020
Price: 500.0 USD
Availability: InStock

2423 Citations

Our Anti-Green Fluorescent Protein (GFP) chicken polyclonal primary antibody from Aves Labs is produced in-house and detects Green Fluorescent Protein (GFP) and related variants including eGFP, YFP, CFP and others. It is a mixture of IgY fraction and affinity-purified antibodies and is great for use in ELISA, IHC, ICC, and WB.

Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red).

Pyramidal neurons in the hippocampal formation of a neonatal mouse brain. Tissue was paraformaldehyde-fixed (4%) and paraffin-embedded. GFP staining is in green in left panel.

Comparison between GFP immunoreactivity in Rexed Lamina 2 neurons of a transgenic mouse using Aves Labs’ anti-GFP (green) and rabbit anti-GFP (red). The transgenic mouse was generated by placing the GFP cDNA after a POMC gene promoter in the transfected plasmid. Mark Zilka, Univ. North Carolina.

Comparison between GFP-immunoreactivity using Aves' anti-GFP antibody (left panel in red) and autofluorescence (right panel in green). In this case, the cortical neuron in this unfixed thick section was first photographed for GFP autofluorescence (left), and then the section was fixed (4% paraformaldehyde) and immunostained for GFP-immunoreactivity (1:1000 dilution) using Texas Red-goat anti-chicken IgY antibodies (Jackson ImmunoResearch) as a secondary. The same cell (left) was then identified.

Immunoreactivity using anti-GFP antibody (left panel in red), Western blot showing specific immunolabeling of the GFP protein.

Immunolabeling of dividing Drosophilia larval neuroblast using Anti-GFP (green, Cat. No. GFP-1010, 1:500) to detect the GFP tagged telomere protein HOAP, and nuclei stained with DAPI (grey). Image was taken with a Zeiss 880 confocal and kindly provided by Brandt Warecki, Sullivan Lab, University of California, Santa Cruz.

Immunostaining of mouse hippocampus identifying injected HSV containing GFP (Cat GFP, 1:250, green) and DAPI (blue). Image kindly provided by Chad Brunswick, Penn State University.

Immunostaining of adult hippocampal neural stem cells from Nestin GFP trangenic mice labeling GFAP (orange, mouse) and GFP (orange, 1:1000, chicken, cat co GFP). The confocal image was obtained from a Leica Sp8 confocal microscope, magnification 40X. Image kindly provided by Soraya Martín Suarez, Achucarro Basque Center for Neuroscience, Spain.

Immunostaining of CX3CR1-Cre mouse hypothalamus showing specific detection of GFP (cat. GFP, green) and IBA1 (red). Nuclei are labeled with DAPI (blue). Image kindly provided by Karmen Ma, NIDDK – National Institutes of Health.

Immunostaining of CX3CR1-Cre mouse hypothalamus showing specific detection of GFP (cat. GFP, green) and IBA1 (red). Image kindly provided by Karmen Ma, NIDDK – National Institutes of Health.

Immunostaining of mouse primary astrocyte cultures expressing a GFP tagged GPCR, stained with GFP (cat. GFP, 1:2500). Image kindly provided by Sandeep Singh, Virginia Commonwealth University.

Representative image defining the following zebrafish microglial morphologies: ramified, cup formation, and soma retraction. White arrowheads show interactions between the microglial process (mpeg:EGFP-CAAX, Cat no GFP, green, 1:1000) and surrounding neuronal cell bodies (NBT:dsRed). Image from publication CC-BY-4.0. PMID: 38490196

Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red).

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: GFP-1020

Volume: 400 µL

Variant

Price:

$500.00

Quantity:

Product Details

Target

Green Fluorescent Protein (GFP)

Target Description

Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions.

Format

IgY Fraction

Concentration

10 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Molecular Weight

27 kDa

Antigen

Recombinant GFP expressed in Escherichia coli

Antibody Registry ID

AB_2307313

Storage

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Physical State

Liquid

Production Notes

Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks and then affinity-purified antibodies were prepared using GFP conjugated to an agarose matrix. The final product is a filter-sterilized mixture of both affinity-purified antibodies (30 µg/mL) and purified IgY (10 mg/mL).

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.

Dilution Ranges

WB: 1:5000-1:10000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000

Quality Control

Antibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product. Western blots were performed using BlokHen® (Aves Labs) as the blocking reagent, and HRP-labeled goat anti-chicken antibodies (Aves Labs, Cat. #H-1004) as the detection reagent. Immunohistochemistry used tetramethyl rhodamine-labeled anti-chicken IgY.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product.

Shipping

Ice packs

Product Specific References for Applications and Species

FISH
Flow Cytometry
Immunocytochemistry
Immunohistochemistry
Western Blot

FISH
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Flow Cytometry
PMID	Dilution	Publication
34732708	1:500	Jarmas, A.E., et al. 2021. Progenitor translatome changes coordinated by Tsc1 increase perception of Wnt signals to end nephrogenesis. Nature Communications, 6332.

Immunocytochemistry
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38845196	1:1000	Hanlon, KS, et al. 2024. In vivo selection in non-human primates identifies AAV capsids for on-target CSF delivery to spinal cord. Molecular therapy : the journal of the American Society of Gene Therapy, 0.
38722021	1:500	Forcella, P, et al. 2024. SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA. eLife, 0.
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38664376	1:500	Liu, X, et al. 2024. Numb positively regulates Hedgehog signaling at the ciliary pocket. Nature Communications, 3365.
38660387	1:500	Järvelä, V, et al. 2024. A novel pathogenic SLC12A5 missense variant in epilepsy of infancy with migrating focal seizures causes impaired KCC2 chloride extrusion. Frontiers in Molecular Neuroscience, 1372662.
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38565145	not listed	Grommisch, D, et al. 2024. Defining the contribution of Troy-positive progenitor cells to the mouse esophageal epithelium. Developmental Cell, 1269-1283.
38454080	1:5000	Viou, L, et al. 2024. PAK3 activation promotes the tangential to radial migration switch of cortical interneurons by increasing leading process dynamics and disrupting cell polarity. Molecular Psychiatry, 0.
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37969757	1:1000	Sun, XY, et al. 2023. Generation of Human Blood Vessel and Vascularized Cerebral Organoids. Bio-protocol, e4870.
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37865312	1:500	Benner, O, et al. 2023. Multiple N-linked glycosylation sites critically modulate the synaptic abundance of Neuroligin isoforms. The Journal of Biological Chemistry, 105361.
37858465	1:1000	Hoi, KK, et al. 2023. Primary cilia control oligodendrocyte precursor cell proliferation in white matter injury via Hedgehog-independent CREB signaling. Cell Reports, 113272.
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Immunohistochemistry
PMID	Dilution	Publication
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39159375	1:500	Tang, R., et al. 2024. Functional dissection of mosquito humidity sensing reveals distinct Dry and Moist Cell contributions to blood feeding and oviposition. PNAS USA, e2407394121.
39152112	1:500	Erickson, A.G., et al. 2024. Motor innervation directs the correct development of the mouse sympathetic nervous system. Nature Communications, 7065.
39147933	1:2000	Liu, Y., et al. 2024. A subset of dopamine receptor-expressing neurons in the nucleus accumbens controls feeding and energy homeostasis. Nature Metabolism, 1616-1631.
39137988	1:2500	Kaneko, T., et al. 2024. Transsynaptic BMP Signaling Regulates Fine-Scale Topography between Adjacent Sensory Neurons. eNeuro, .
39117691	1:2000	Rybiczka-Tešulov, M., et al. 2024. Circular RNAs regulate neuron size and migration of midbrain dopamine neurons during development. Nature Communications, 6773.
39107057	1:1000	Gonye, E.C., et al. 2024. Intrinsic Molecular Proton Sensitivity Underlies GPR4 Effects on Retrotrapezoid Nucleus Neuronal Activation and CO2-Stimulated Breathing. The Journal of Neuroscience, .
39104322	1:500	Bu, H.F., et al. 2024. A novel mouse model for hepatocyte-specific apoptosis-induced myeloid cell-dominant sterile liver injury and repair. American Journal of Physiology. Gastrointestinal and Liver Physiology, .
39095832	1:500	Hu, X., et al. 2024. Lactate promotes microglial scar formation and facilitates locomotor function recovery by enhancing histone H4 lysine 12 lactylation after spinal cord injury. Journal of Neuroinflammation, 193.
39095587	1:1000-1:5000	Decoster, L., et al. 2024. A GnRH neuronal population in the olfactory bulb translates socially relevant odors into reproductive behavior in male mice. Nature Neuroscience, 1758-1773.
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38996064	1:500	Visser, J., et al. 2024. Astroglial networks control visual responses of superior collicular neurons and sensory-motor behavior. Cell Reports, 114504.
38994990	1:1000	Pose-Méndez, S., et al. 2024. In Vivo Monitoring of Fabp7 Expression in Transgenic Zebrafish. Cells, .
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38961289	1:500	Theparambil, S.M., et al. 2024. Adenosine signalling to astrocytes coordinates brain metabolism and function. Nature, 139-146.
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38884422	1:1000	Zhang, X, et al. 2024. The astrocyte-enriched gene deathstar plays a crucial role in the development, locomotion, and lifespan of D. melanogaster. Fly, 2368336.
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38849523	1:2000	Holl, D, et al. 2024. Distinct origin and region-dependent contribution of stromal fibroblasts to fibrosis following traumatic injury in mice. Nature neuroscience, 0.
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38838158	1:3000	Singh, A, et al. 2024. Gene regulatory landscape of cerebral cortex folding. Science advances, eadn1640.
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38678557	1:1000	Buchan, MJ, et al. 2024. Higher-order thalamocortical circuits are specified by embryonic cortical progenitor types in the mouse brain. Cell Reports, 114157.
38672522	1:200	Furuno, K, et al. 2024. Transduction and Genome Editing of the Heart with Adeno-Associated Viral Vectors Loaded onto Electrospun Polydioxanone Nonwoven Fabrics. Biomolecules, 0.
38657057	1:1000	Rein, B, et al. 2024. MDMA enhances empathy-like behaviors in mice via 5-HT release in the nucleus accumbens. Science Advances, eadl6554.
38649521	1:500	Liu, M, et al. 2024. Dietary cysteine and methionine promote peroxisome elevation and fat loss by induction of CG33474 expression in Drosophila adipose tissue. Cellular and Molecular Life Sciences, 190.
38607918	1:1000	Tamboli, S, et al. 2024. Mouse hippocampal CA1 VIP interneurons detect novelty in the environment and support recognition memory. Cell Reports, 114115.
38607913	1:200	Sun, J, et al. 2024. ptx3a+ fibroblast/epicardial cells provide a transient macrophage niche to promote heart regeneration. Cell Reports, 114092.
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38592453	1:250	Trotter, TN, et al. 2024. Overcoming Xenoantigen Immunity to Enable Cellular Tracking and Gene Regulation with Immune-competent "NoGlow" Mice. Cancer Research Communications, 1050-1062.
38589390	1:4000	Gau, YA, et al. 2024. Multicore fiber optic imaging reveals that astrocyte calcium activity in the mouse cerebral cortex is modulated by internal motivational state. Nature Communications, 3039.
38582915	1:1000	Ferreira, A, et al. 2024. Distinct forms of structural plasticity of adult-born interneuron spines in the mouse olfactory bulb induced by different odor learning paradigms. Communications Biology, 420.
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Western Blot
PMID	Dilution	Publication
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Anti-Green Fluorescent Protein Antibody

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