Anti-Gs Protein, Alpha Subunit Antibody (N192/12)

Name: Anti-Gs Protein, Alpha Subunit Antibody
Brand: NeuroMab
SKU: 75-211
Price: 329.0 USD
Availability: InStock

8 Citations

Our Anti-Gs protein, alpha subunit mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N192/12. It detects mouse and rat Gs protein, alpha subunit, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Adult rat brain membrane immunoblot.

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Species Reactivity

Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: 75-211

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Product Details

Target

Gs protein, alpha subunit

Target Description

Guanine nucleotide-binding protein G(s) subunit alpha isoforms short or Gs protein α subunit is encoded by the gene GNAS. Gsα is one of three subunits that make up a guanine nucleotide binding protein (Gprotein). The other proteins are a beta and a gamma subunit. G proteins are further divided into families depending on the type of α subunit included, G(S)alpha,G(Q)alpha,G(I)alpha,and G(12)alpha. Gsα functions as a transducer of numerous signaling pathways that are controlled by g protein coupled receptors (GPCRs) by coupling receptors to adenylyl cyclase and results in increased levels of the signaling molecule cAMP. Diseases associated with GNAS include Mccune-Albright Syndrome and Progressive Osseous Heteroplasia.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N192/12

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

GNAS GNAS1

Molecular Weight

45 kDa

Antigen

Fusion protein amino acids 1-380 (full-length, short form) of bovine Gs protein alpha subunit (accession number P04896-2) produced recombinantly in E. Coli

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2315846

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Reacts with both Active and Inactive/QL forms

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (Adenylate cyclase-stimulating G alpha protein)

UniProt ID

P04896-2

Product Specific References for Applications and Species

Immunocytochemistry: Rat

Immunoprecipitation: Rat

Western Blot: Human | Mouse | Rat

Additional Publications: Unspecified

Immunocytochemistry: Rat
PMID	Dilution	Publication
25691569	not listed	Sarma, T., et al. 2015. Activation of microtubule dynamics increases neuronal growth via the nerve growth factor (NGF)- and Gαs-mediated signaling pathways. Journal of Biological Chemistry, 10045-10056.

Immunoprecipitation: Rat
PMID	Dilution	Publication
30617260	not listed	Singh, H., et al. 2019. Disruption of lipid-raft localized Gαs/tubulin complexes by antidepressants: a unique feature of HDAC6 inhibitors, SSRI, and tricyclic compounds. Neuropsychopharmacology, 1008.

Western Blot: Human
PMID	Dilution	Publication
32327735	1:400	Chukaew, P., et al. 2020. Potential depression and antidepressant-response biomarkers in human lymphoblast cell lines from treatment-responsive and treatment-resistant subjects: roles of SSRIs and omega-3 polyunsaturated fatty acids. Molecular Psychiatry, 2402-2414.
22940628	not listed	Sun, Z., et al. 2012. The Gβ3 splice variant associated with the C825T gene polymorphism is an unstable and functionally inactive protein.. Cellular Signalling, 2349-2359.

Western Blot: Mouse
PMID	Dilution	Publication
22933780	1:200	Loo, L., et al. 2012. The C-type natriuretic peptide induces thermal hyperalgesia through a noncanonical Gβγ-dependent modulation of TRPV1 channel.. Journal of Neuroscience, 11942-11955.

Western Blot: Rat
PMID	Dilution	Publication
29895894	not listed	Wray, N.H., et al. 2019. NMDAR-independent, cAMP-dependent antidepressant actions of ketamine. Molecular Psychiatry, 1833-1843.
26162823	not listed	Donati, R.J., et al. 2015. Differential effects of antidepressants escitalopram versus lithium on Gs alpha membrane relocalization. BMC Neuroscience, 40.

Additional Publications: Unspecified
PMID	Publication
21811789	Soltysinska, E., et al. 2011. Chronic sympathetic activation promotes downregulation of β-adrenoceptor-mediated effects in the guinea pig heart independently of structural remodeling and systolic dysfunction.. Pflügers Archiv: European Journal of Physiology, 529-543.

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Anti-Gs Protein, Alpha Subunit Antibody (N192/12)

SKU: 75-211

Product Details

Product Specific References for Applications and Species

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