Anti-GST Antibody (N100/13)

15 Citations

Our Anti-GST mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N100/13. It detects GST, and is purified by Protein A chromatography. It is great for use in ELISA, ICC, WB.

Bacterially expressed GST fusion protein immunoblot.
Bacterially expressed GST fusion protein immunoblot.
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Bacterially expressed GST fusion protein immunoblot.

ELISA, ICC, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet

SKU: 75-148

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

GST
6xHIS or His-tag is a commonly used recombinant tag that consists of 6 sequential histidine residues placed at the amino or carboxy terminus of a targeted sequence. 6xHis is useful for purification of proteins or visualization by western blot or immunocytochemistry.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N100/13
IgG1
ELISA, ICC, WB
Mouse
26 kDa
Identified as off-target mAb in screen for anti-Stargazin Neuromabs (immunogen was GST fusion protein amino acids 204-323, cytoplasmic C-terminus, of mouse TARPGamma2). Target identified as GST by ELISA and immunoblot against other GST fusion proteins.
AB_10671818
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.75
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Glutathione S-transferase class-mu 26 kDa isozyme (GST 26) (EC 2.5.1.18) (Sj26 antigen) (SjGST)
P08515

Product Specific References for Applications and Species

ELISA
PMID Dilution Publication
31328160not listedZhao, X., et al. 2019. Fc receptor-like 1 intrinsically recruits c-Abl to enhance B cell activation and function. Science Advances, .
Flow Cytometry
PMID Dilution Publication
23487040not listedBarry, J., et al. 2013. Activation of conventional kinesin motors in clusters by Shaw voltage-gated K+ channels.. Journal of Cell Science, 2027-41.
Immunoprecipitation
PMID Dilution Publication
29861105not listedWang, C.C., et al. 2018. βIV Spectrinopathies Cause Profound Intellectual Disability, Congenital Hypotonia, and Motor Axonal Neuropathy. American Journal of Human Genetics, 1158-1168.
287061961ug/ulWilkinson, B., et al. 2017. Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling. Scientific Reports, 5272.
23676497not listedMarcello, E., et al. 2013. Endocytosis of synaptic ADAM10 in neuronal plasticity and Alzheimer''s disease.. Journal of Clinical Investigation, 2523-38.
Western Blot
PMID Dilution Publication
35434559not listedGupta, S., et al. 2022. The non-adrenergic imidazoline-1 receptor protein nischarin is a key regulator of astrocyte glutamate uptake. iScience, 104127.
35312684not listedSchmerl, B., et al. 2022. The synaptic scaffold protein MPP2 interacts with GABAA receptors at the periphery of the postsynaptic density of glutamatergic synapses. PLoS Biology, e3001503.
319003871:2000Hamdan, H., et al. 2020. Mapping axon initial segment structure and function by multiplexed proximity biotinylation. Nature Communications, 100.
317750311:500Halff, E.F., et al. 2019. SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling. Cell Reports, 2599-2607.
31069631not listedCarrano, N., et al. 2019. The synaptonuclear messenger RNF10 acts as an architect of neuronal morphology.. Molecular Neurobiology, 7583-7593.
25362473not listedHo, T.S., et al. 2015. A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier.. Nature Neuroscience, 1664-72.
23430259not listedOkamoto, Y., et al. 2013. Role of C-terminal membrane-proximal basic residues in cell surface trafficking of HIV coreceptor GPR15 protein.. The Journal of Biological Chemistry, 9189-99.
23213412not listedSegal, M., et al. 2012. Ndel1-derived peptides modulate bidirectional transport of injected beads in the squid giant axon.. Biol Open., 220-31.
Unspecified
PMID Publication
23772394Bernstein, J.D., et al. 2013. Potential use of potassium efflux-deficient yeast for studying trafficking signals and potassium channel functions.. FEBS Open Bio., 196-203.
20980075Marcello, E., et al. 2012. SAP97-mediated local trafficking is altered in Alzheimer disease patients'' hippocampus.. Neurobiology of Aging, e1-10.

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