Anti-HCN1 Antibody (N70/28)
Our Anti-HCN1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N70/28. It is KO validated, detects human, mouse, and rat HCN1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
Adult rat and mouse brain membrane (RBM and MBM, respectively) and transfected cell immunoblot: extracts of RBM, HCN1-KO and -WT MBM and COS-1 cells transfected with GFP-HCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N70/28 (left panel) or K89/34 TC supe (right panel).
Gerbil, Human, Mouse, Non-Human Primate, Rat
EM, ICC, IHC, IP, WB
Mouse
KO Validated
Trial Size
Product Specific References for Applications and Species
- Electron Microscopy: Mouse
- Immunocytochemistry: Mouse | Rat
- Immunohistochemistry: Avian | Gerbil | Human | Mouse | Non-Human Primate | Rat
- Immunoprecipitation: Mouse
- Western Blot: Mouse | Rat
- Additional Publications: Unspecified
| Electron Microscopy: Mouse | ||
| PMID | Dilution | Publication |
| 23077068 | 1:100 | Huang, Z., et al. 2012. TRIP8b-independent trafficking and plasticity of adult cortical presynaptic HCN1 channels.. Journal of Neuroscience, 14835-14848. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 25201528 | 1:50 | Kupferman, J.V., et al. 2014. Reelin signaling specifies the molecular identity of the pyramidal neuron distal dendritic compartment.. Cell, 1335-1347. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 32248813 | 1:100 | Vay, S.U., et al. 2020. The impact of hyperpolarization-activated cyclic nucleotide-gated (HCN) and voltage-gated potassium KCNQ/Kv7 channels on primary microglia function. Journal of Neuroinflammation, 100. |
| 23516351 | 1:200 | Abbas, S.Y., et al. 2013. Directional summation in non-direction selective retinal ganglion cells.. PLOS Computational Biology, e1002969. |
| Immunohistochemistry: Avian | ||
| PMID | Dilution | Publication |
| 36550622 | 1:500 | Balaji, V., et al. 2023. Immunohistochemical characterization of bipolar cells in four distantly related avian species. Journal of Comparative Neurology, 561-581. |
| Immunohistochemistry: Gerbil | ||
| PMID | Dilution | Publication |
| 27110919 | not listed | Ko, K.W., et al. 2016. Serotonin modulates spike probability in the axon initial segment through HCN channels. Nature Neuroscience, 826-834. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 33208499 | 1:750 | Hsieh, L.S., et al. 2020. Ectopic HCN4 expression drives mTOR-dependent epilepsy in mice. Science Translational Medicine, eabc1492. |
| 24107939 | 1:1000 | Puthussery, T., et al. 2013. NaV1. 1 channels in axon initial segments of bipolar cells augment input to magnocellular visual pathways in the primate retina. . Journal of Neuroscience, 16045-16059. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 39406481 | 1:1000 | Herstel, LJ, et al. 2024. Distinct modulation of Ih by synaptic potentiation in excitatory and inhibitory neurons. eNeuro, . |
| 35972069 | 1:300 | Merseburg, A., et al. 2022. Seizures, behavioral deficits, and adverse drug responses in two new genetic mouse models of HCN1 epileptic encephalopathy. Elife, e70826. |
| 35437278 | 1:00 | Lankford, C.K., et al. 2022. Cone-driven retinal responses are shaped by rod but not cone HCN1. Journal of Neuroscience, 4231-4249. |
| 33502442 | 1:5 (supe) | Fortenbach, C., et al. 2021. Loss of the K+ channel Kv2. 1 greatly reduces outward dark current and causes ionic dysregulation and degeneration in rod photoreceptors. Journal of General Physiology, e202012687. |
| 33105624 | 1:200 | Salling, M.C., et al. 2020. Constitutive Genetic Deletion of Hcn1 Increases Alcohol Preference during Adolescence. Brain Sciences, 763. |
| 31694913 | not listed | Datta, P., et al. 2019. The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors. Journal of Biological Chemistry, 19119-19136. |
| 31555090 | 1:100-1:200 | Tetenborg, S., et al. 2019. Localization of Retinal Ca2+/Calmodulin-Dependent Kinase II-β (CaMKII-β) at Bipolar Cell Gap Junctions and Cross-Reactivity of a Monoclonal Anti-CaMKII-β Antibody With Connexin36. Frontiers in Molecular Neuroscience, 206. |
| 30943094 | not listed | Naumov, V., et al. 2019. Analysis of excitatory and inhibitory neuron types in the inferior colliculus based on Ih properties. Journal of Neurophysiology, 2126-2139. |
| 29915134 | 1:200 | Salling, M.C., et al. 2018. Alcohol consumption during adolescence in a mouse model of binge drinking alters the intrinsic excitability and function of the prefrontal cortex through a reduction in …. Journal of Neuroscience, 6207-6222. |
| 28071723 | 1-2 ug/ml | Huang, Z., et al. 2017. HCN1 channels reduce the rate of exocytosis from a subset of cortical synaptic terminals. Scientific Reports, 40257. |
| 27277803 | 1:500 | Knoll, A.T., et al. 2016. Quantitative trait loci and a novel genetic candidate for fear learning. Journal of Neuroscience, 6258-6268. |
| 26704635 | not listed | Hellmer, C.B., et al. 2016. Morphological and physiological analysis of type-5 and other bipolar cells in the Mouse Retina.. Neuroscience, 246-258. |
| 26587737 | 1:200 | Farshi, P., et al. 2016. Dopamine D1 receptor expression is bipolar cell type-specific in the mouse retina.. Journal of Comparative Neurology, 2059-2079. |
| 26269648 | not listed | Kole, M.J., et al. 2015. Selective loss of presynaptic potassium channel clusters at the cerebellar basket cell terminal pinceau in adam11 mutants reveals their role in ephaptic control of purkinje cell firing. . Journal of Neuroscience, 11433-11444. |
| 24558054 | 1:100 | Liu, Q., et al. 2014. I h and HCN channels in murine spiral ganglion neurons: tonotopic variation, local heterogeneity, and kinetic model.. Journal of the Association for Research in Otolaryngology, 585-599. |
| 24409334 | 1:500 | Pan, Y., et al. 2014. TRIP8b is required for maximal expression of HCN1 in the mouse retina.. PLoS One, e85850. |
| 24000178 | 1:1000 | Rinaldi, A., et al. 2013. HCN1 channels in cerebellar Purkinje cells promote late stages of learning and constrain synaptic inhibition.. The Journal of Physiology, 5691-5709. |
| 21555075 | not listed | Piskorowski, R., et al. 2011. TRIP8b splice forms act in concert to regulate the localization and expression of HCN1 channels in CA1 pyramidal neurons.. Neuron, 495-509. |
| Immunohistochemistry: Non-Human Primate | ||
| PMID | Dilution | Publication |
| 24107939 | 1:1000 | Puthussery, T., et al. 2013. NaV1. 1 channels in axon initial segments of bipolar cells augment input to magnocellular visual pathways in the primate retina. . Journal of Neuroscience, 16045-16059. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 36395691 | 1:500 | Choi, K., et al. 2022. Presynaptic HCN Channel Activity Is Required For The Expression Of Long-Term Potentiation At Lateral Amygdala To Basal Amygdala Synapses. Biochemical And Biophysical Research Communications, 100-107. |
| 34670009 | 1ug/ml | Michaelson, S.D., et al. 2021. Long‐Lived Organotypic Slice Culture Model of the Rat Basolateral Amygdala. Current Protocols, e267. |
| 29650696 | 0.5ug/ml | Villarroel, H.S., et al. 2018. NPY induces stress resilience via downregulation of Ih in principal neurons of rat basolateral amygdala. Journal of Neuroscience, 4505-4520. |
| 26760498 | 1:1000 | Gessele, N., et al. 2016. Structural Changes and Lack of HCN1 Channels in the Binaural Auditory Brainstem of the Naked Mole-Rat (Heterocephalus glaber).. PLoS One, e0146428. |
| 22832570 | not listed | Almanza, A., et al. 2012. Molecular identity, ontogeny, and cAMP modulation of the hyperpolarization-activated current in vestibular ganglion neurons.. Journal of Neurophysiology, 2264-2275. |
| 21945537 | 1:300 | Kanyshkova, T., et al. 2012. Differential regulation of HCN channel isoform expression in thalamic neurons of epileptic and non-epileptic rat strains.. Neurobiology of Disease, 450-461. |
| 21938402 | 1:1000 | Anderson, W.D., et al. 2011. Properties and functional implications of I (h) in hippocampal area CA3 interneurons.. Pflügers Archiv, 895-912. |
| 21456027 | 1ug/ml | Stradleigh, T.W., et al. 2011. Colocalization of hyperpolarization‐activated, cyclic nucleotide‐gated channel subunits in rat retinal ganglion cells. Journal of Comparative Neurology, 2546-2573. |
| Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 32467357 | 1:4 (10ug) | Brandalise, F., et al. 2020. Fragile X mental retardation protein bidirectionally controls dendritic ih in a cell type-specific manner between mouse hippocampus and prefrontal cortex. Journal of Neuroscience, 5327-5340. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 39088003 | 1:500 | Lai, H.J., et al. 2024. Increase of HCN current in SOD1-associated amyotrophic lateral sclerosis. Brain, . |
| 34429357 | 1:500-1:2000 | Schlusche, A.K., et al. 2021. Developmental HCN channelopathy results in decreased neural progenitor proliferation and microcephaly in mice. PNAS: USA, e2009393118. |
| 33502442 | 1:10 (supe) | Fortenbach, C., et al. 2021. Loss of the K+ channel Kv2. 1 greatly reduces outward dark current and causes ionic dysregulation and degeneration in rod photoreceptors. Journal of General Physiology, e202012687. |
| 32347567 | not listed | Park, H., et al. 2020. Splice‐dependent trans‐synaptic PTP δ–IL 1 RAPL 1 interaction regulates synapse formation and non‐REM sleep. EMBO Journal, e104150. |
| 29906573 | not listed | Musial, T.F., et al. 2018. Store depletion-induced h-channel plasticity rescues a channelopathy linked to Alzheimer's disease. Neurobiology of Learning and Memory, 141-157. |
| 28467171 | 1:300 | Fan, J., et al. 2017. Down-regulation of T-type Cav3. 2 channels by hyperpolarization-activated cyclic nucleotide-gated channel 1 (HCN1): Evidence of a signaling complex. Channels, 434-443. |
| 27047338 | 1:500 | Fan, J., et al. 2016. Reduced Hyperpolarization-Activated Current Contributes to Enhanced Intrinsic Excitability in Cultured Hippocampal Neurons from PrP−/− Mice. Frontiers in Cellular Neuroscience, 74. |
| 26269648 | 1:200 | Kole, M.J., et al. 2015. Selective loss of presynaptic potassium channel clusters at the cerebellar basket cell terminal pinceau in adam11 mutants reveals their role in ephaptic control of purkinje cell firing. . Journal of Neuroscience, 11433-11444. |
| 25383903 | 1:00 | Zhang, Y., et al. 2014. Dendritic channelopathies contribute to neocortical and sensory hyperexcitability in Fmr1(-/y) mice.. Nature Neuroscience, 1701-1703. |
| 23516534 | 1:1000 | Bonin, R.P., et al. 2013. Hyperpolarization-Activated Current (Ih) Is Reduced in Hippocampal Neurons from Gabra5−/− Mice. PLoS One, e58679. |
| 22880098 | not listed | Ying, S.W., et al. 2012. Targeted deletion of Kcne2 impairs HCN channel function in mouse thalamocortical circuits.. PLoS One, e42756. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 30053369 | not listed | McClatchy, D.B., et al. 2018. Structural Analysis of Hippocampal Kinase Signal Transduction. ACS Chemical Neuroscience, 3072-3085. |
| 29427087 | 1:1000 | Frigerio, F., et al. 2018. Neuroinflammation Alters Integrative Properties of Rat Hippocampal Pyramidal Cells. Molecular Neurobiology, 7500-7511. |
| 26760498 | 1:1000 | Gessele, N., et al. 2016. Structural Changes and Lack of HCN1 Channels in the Binaural Auditory Brainstem of the Naked Mole-Rat (Heterocephalus glaber).. PLoS One, e0146428. |
| 23536771 | 1:1000 | Brewster, A.L., et al. 2013. Rapamycin reverses status epilepticus-induced memory deficits and dendritic damage.. PLoS One, e57808. |
| 21905079 | 1:1000 | McClelland, S., et al. 2011. Neuron-restrictive silencer factor-mediated hyperpolarization-activated cyclic nucleotide gated channelopathy in experimental temporal lobe epilepsy.. Annals of Neurology, 454-464. |
| 21456027 | 2ug/ml | Stradleigh, T.W., et al. 2011. Colocalization of hyperpolarization‐activated, cyclic nucleotide‐gated channel subunits in rat retinal ganglion cells. Journal of Comparative Neurology, 2546-2573. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 39025677 | Bompolaki, M., et al. 2024. Activation of Neuropeptide Y (NPY) receptors in the basolateral amygdala (BLA) inhibits projections to the bed nucleus of the stria terminalis (BNST) and buffers stress-induced decreases in social interaction in male rats. The Journal of Neuroscience, . | |
| 26559914 | Masri, R.A., et al. 2016. Connectivity between the OFF bipolar type DB3a and six types of ganglion cell in the marmoset retina. Journal of Comparative Neurology, 1839-1858. | |
| 25262625 | Weltzien, F., et al. 2015. Analysis of bipolar and amacrine populations in marmoset retina.. Journal of Comparative Neurology, 313-334. | |
| 21358644 | Huang, Z., et al. 2011. Presynaptic HCN1 channels regulate Cav3.2 activity and neurotransmission at select cortical synapses.. Nature Neuroscience, 478-486. | |
| 21159967 | Giesbrecht, C.J., et al. 2010. Countervailing modulation of Ih by neuropeptide Y and corticotrophin-releasing factor in basolateral amygdala as a possible mechanism for their effects on stress-related behaviors.. Journal of Neuroscience, 16970-16982. | |
| 20589908 | Li, Y., et al. 2010. Synaptic and nonsynaptic localization of protocadherin-gammaC5 in the rat brain.. Journal of Comparative Neurology, 3439-3463. | |
| 20215108 | Noam, Y., et al. 2010. Trafficking and surface expression of hyperpolarization-activated cyclic nucleotide-gated channels in hippocampal neurons.. The Journal of Biological Chemistry, 14724-14736. | |
| 20130205 | Hegle, A.P., et al. 2010. Evolutionary emergence of N-glycosylation as a variable promoter of HCN channel surface expression.. American Journal of Physiology-Cell Physiology, C1066-C1076. | |
| 19563776 | Arimitsu, T., et al. 2009. Activity-dependent regulation of HCN1 protein in cortical neurons.. Biochemical and Biophysical Research Communications, 87-91. | |