Our IκBα (C-terminus) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat IκBα (C-terminus) and is antigen affinity purified. It is great for use in WB.
Western blot image of human A431. The Blots were probed with anti-IκBα (C-term.) polyclonal antibody at a dilution of 1:500 (lane 1), 1:1000 (lane 2), and 1:2000 (lane 3).
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation of IκBα occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-κB. This pathway of IκBα regulation occurs in response to various NF-κB-activating agents, such as TNFα, interleukins, LPS, and irradiation. An alternative pathway for IκBα regulation occurs through tyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IκBα and NF-κB-activation. In contrast, Tyr-305 phosphorylation by c-Abl has been implicated in IκBα nuclear translocation and inhibition of NF-κB-activation. Thus, tyrosine phosphorylation of IκBα may be an important regulatory mechanism in NF-κB signaling.
Antigen Affinity Purified
Polyclonal
IgG
WB
Rabbit
NFKBIA
38
IκBα (C-terminus) synthetic peptide (coupled to KLH) corresponding to amino acid residues at the C-terminus of human IκBα. This peptide sequence has high homology to rat and mouse IκBα, and low homology to other IκB proteins.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 0.05% NaN3 and 50% glycerol
WB: 1:1000
Unconjugated
This antibody detects a 38 kDa* protein on SDS-PAGE immunoblots of human A431 and Jurkat cells, as well as mouse C2C12 cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Zhang, Y, et al. 2018. Exogenous oxidants activate nuclear factor kappa B through Toll-like receptor 4 stimulation to maintain inflammatory phenotype in macrophage. Biochemical pharmacology, 104-118.
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