Anti-Integrin β4 (Tyr-1494), Phosphospecific Antibody

Name: Anti-Integrin β4 (Tyr-1494), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: IP1281
Price: 329.0 USD
Availability: InStock

6 Citations

Our Integrin β4 (Tyr-1494) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Integrin β4 (Tyr-1494) and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical labeling of integrin β4 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-integrin β4 (Cytoplasmic region) (left) or rabbit polyclonals anti-integrin β4 (Tyr-1494) (middle) or anti-integrin β4 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight® 594.

Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-Integrin β4 (Tyr-1526) (lanes 1 & 2) and anti-Integrin β4 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-Integrin β4 (lanes 5 & 6).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: IP1281

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Product Details

Target

Integrin β4 (Tyr-1494)

Target Description

Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The integrin β2 subunit associates with integrin αL to form a receptor for ICAM family members. Integrin β2/αL is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

ITGB4

Molecular Weight

200

Antigen

Phospho-Integrin β4 (Tyr-1494) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1494 of human Integrin β4. This peptide sequence is found in all three Integrin β4 isoforms and is highly conserved in rat and mouse Integrin β4.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:2000
ICC: 1:100

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-Integrin β4 (Tyr-1494) peptide (without carrier). The antibody detects a 200kDa* protein corresponding to the molecular mass of Integrin β4 on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but not in control cells. Similar results were also observed in src-transformed Hct116 cells treated with pervanadate.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-1494

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

integrin, CD104, GP150

UniProt Details

UniProt (Human): P16144-2
UniProt (Immunogen Species): P16144-2

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
28011194	not listed	Dilly, AK, et al. 2017. Convergence of eicosanoid and integrin biology: Role of Src in 12-LOX activation. Experimental cell research, 1-10.
25938910	not listed	Coleman, D.T., et al. 2015. Curcumin Prevents Palmitoylation of Integrin β4 in Breast Cancer Cells. PLoS One, e0125399.
24180592	not listed	Soung, Y.H., et al. 2013. The role of c-Src in integrin (α6β4) dependent translational control. BMC Cell Biology, 49.
21388972	not listed	Soung, Y.H., et al. 2011. Curcumin inhibition of the functional interaction between integrin α6β4 and the epidermal growth factor receptor. Molecular Cancer Therapy, 883-891.
20855525	not listed	Yang, X., et al. 2010. SHP2 mediates the localized activation of Fyn downstream of the α6β4 integrin to promote carcinoma invasion. Molecular and Cellular Biology, 5306-5317.
19138983	not listed	Kim, H.I., et al. 2008. Curcumin inhibition of integrin (alpha6beta4)-dependent breast cancer cell motility and invasion. Cancer Prevention Research, 385-391.

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Anti-Integrin β4 (Tyr-1494), Phosphospecific Antibody

SKU: IP1281

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Integrin β4 (Tyr-1494), Phosphospecific Antibody

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