Our Anti-KCC2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N1/12. It detects human, mouse, rat, and zebrafish KCC2, and is purified by Protein A chromatography. It is great for use in IHC, ICC.
KCC2 or Potassium-chloride transporter member 5 is a member of the chloride potassium symporter family. KCC2 is a neuron-specific membrane protein encoded by the SLC12A5 gene in humans. The protein colocalizes with GABAA receptors and has for function to mediates electroneutral potassium-chloride cotransport in mature neurons to mainatin the Cl- homeostasis. KCC2 is widely expressed throughout the central nervous system, especially the hippocampus, hypothalamus, brainstem, and motoneurons of the ventral spinal cord. KCC2 is a neuronal marker that is often used to measure post-synaptic inhibition through GABAA receptors.
Purified by Protein A chromatography
0.5 mg/mL
Monoclonal
N1/12
IgG2a
ICC, IHC
Mouse
Slc12a5 Kcc2 Kiaa1176
140 kDa
Fusion protein amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2 (accession number Q63633) produced recombinantly in E. Coli
Human, Mouse, Rat, Zebrafish
AB_2939115
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
PBS with 0.09% azide
FL650 Ex: 655 nm, Em: 676 nm
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from date of receipt
Shipped on ice packs
Solute carrier family 12 member 5 (Electroneutral potassium-chloride cotransporter 2) (K-Cl cotransporter 2) (mKCC2) (Neuronal K-Cl cotransporter)
