This antibody is the same product as PhosphoSolutions' catalog number 1006-Ki67, which has been discontinued.
Our Anti-Ki-67 primary antibody from Aves Labs is chicken polyclonal. It detects human, mouse, and rat Ki-67 and is total IgY fraction. It is great for use in WB, IHC, ICC.
Immunostaining of HeLa cells showing specific labeling of Ki-67 (cat. Ki67-0100, 1:2000, red) present in cytoplasmic microtubules. Additional immunostaining done with β-tubulin in green and nuclear staining with DAPI (blue). During cell cycle Ki-67 protein is predominantly expressed in the nucleoli of cells during mitosis and interphase, and is not present during quiescence.
Ki-67 is a nuclear protein that is considered to be essential for cellular proliferation since it is expressed only in active phases of the cell cycle (G1, S, G2 and mitosis) but not in resting phase G0 (Scholzen & Gerdes 2000). Ki-67 antibodies have therefore been widely used as markers of cell growth. Ki-67 has further been identified as a predicative and prognostic marker in breast, lung, cervical and other cancers (Yerushalmi et al., 2010; Folescu et al., 2018; Das et al., 2018).
Total IgY fraction
Polyclonal
IgY
ICC, IHC, WB
Chicken
MKI67
345-395 kDa
Recombinant human ki-67 protein expressed in and purified from E. Coli.
Human, Mouse, Rat
AB_2941673
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Total IgY fraction
PBS + 10 mM NaN3
WB: 1:5000
IHC: 1:1000-1:5000
ICC: 1:1000-1:5000
Unconjugated
Specific for endogenous levels of the ~345 & 395 kDa Ki-67 isoforms. Additional bands are thought to be breakdown products of these 2 isoforms.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
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Scholzen, T. and Gerdes, J., 2000. The Ki‐67 protein: from the known and the unknown. Journal of Cellular Physiology, 182(3), pp.311-322. PMID: 10653597
Yerushalmi, R., Woods, R., Ravdin, P.M., Hayes, M.M. and Gelmon, K.A., 2010. Ki67 in breast cancer: prognostic and predictive potential. The Lancet Oncology, 11(2), pp.174-183. PMID: 20152769
Folescu, R., Levai, C.M., GRIGORAŞ, M.L., Arghirescu, T.S., TALPOŞ, I.C., Gindac, C.M., ZAMFIR, C.L., Poroch, V. and Anghel, M.D., 2018. Expression and significance of Ki-67 in lung cancer. Romanian Journal of Morphology and Embryology, 59(1), pp.227-233. PMID: 29940632
Das, D., Sengupta, M., Basu, K., Tirkey, M., Datta, C. and Chatterjee, U., 2018. Role of p16/Ki-67 Dual immunostaining in detection of cervical cancer precursors. Journal of Cytology, 35(3), p.153-158. PMID: 30089944
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