Anti-Kv1.1 K+ Channel Antibody (K20/78)
Our Anti-Kv1.1 K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K20/78. It is KO validated, detects finch, human, mouse, rabbit, and rat Kv1.1 K+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
Immunolabeling of postnatal 14 mouse hippocampus specifically labeling Kv1.1 (Cat no 75-007, 1:200, green) and Ankyrin G (red). DNA labeled with DAPI. Mice were transcardially perfused with ice-cold 4% paraformaldehyde in PBS, then brain removed and postfixed overnight. The 50 uM slices were floating sections, where antigen retrieval was performed with sodium citrate at 80 C for 30 minutes before immunolabeling. Images from J. Ramirez-Franco and O. El Far, Aix-Marseille Université and INSERM.
Finch, Human, Mouse, Rabbit, Rat
ICC, IHC, IP, WB
Mouse
KO Validated
Product Specific References for Applications and Species
- Affinity Purification: Mouse
- Immunocytochemistry: Human | Mouse | Rabbit | Rat
- Immunohistochemistry: Human | Mouse | Rat | Zebra Finch
- Immunoprecipitation: Mouse
- Western Blot: Human | Mouse | Rat
- Additional Publications: Unspecified
| Affinity Purification: Mouse | ||
| PMID | Dilution | Publication |
| 22067099 | not listed | Bildl, W., et al. 2012. Extending the dynamic range of label-free mass spectrometric quantification of affinity purifications.. Molecular & Cellular Proteomics, . |
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 31586945 | 1:50 | Verdura, E., et al. 2020. Complete loss of KCNA1 activity causes neonatal epileptic encephalopathy and dyskinesia. . Journal of Medical Genetics, 132-137. |
| 23774215 | not listed | Lallet-Daher, H., et al. 2013. Potassium channel KCNA1 modulates oncogene-induced senescence and transformation.. Cancer Research, 5253-5265. |
| 20580615 | 1:50 | Lai, M., et al. 2010. Investigation of LGI1 as the antigen in limbic encephalitis previously attributed to potassium channels: a case series.. The Lancet Neurology, 776-785. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 39037413 | 1:500 | Si, M., et al. 2024. Epilepsy-associated Kv1.1 channel subunits regulate intrinsic cardiac pacemaking in mice. The Journal of General Physiology, . |
| 33689679 | 1:10 | Chang, K.J., et al. 2021. TDP-43 maximizes nerve conduction velocity by repressing a cryptic exon for paranodal junction assembly in Schwann cells. . Elife, e64456. |
| 33168717 | not listed | Cho, I.H., et al. 2020. The potassium channel subunit Kvβ1 serves as a major control point for synaptic facilitation. . Proceedings of the National Academy of Sciences, 29937-29947. |
| 24367293 | 15 μg/ml | Kirchheim, F., et al. 2013. Regulation of action potential delays via voltage-gated potassium Kv1.1 channels in dentate granule cells during hippocampal epilepsy.. Frontiers in Neuroscience, 248. |
| 22641786 | 1:1000 | Glasscock, E., et al. 2012. Transcompartmental reversal of single fibre hyperexcitability in juxtaparanodal Kv1.1-deficient vagus nerve axons by activation of nodal KCNQ channels.. The Journal of Physiology, 3913-3926. |
| 21632923 | not listed | Buttermore, E.D., et al. 2011. The cytoskeletal adaptor protein band 4.1B is required for the maintenance of paranodal axoglial septate junctions in myelinated axons.. Journal of Neuroscience, 8013-24. |
| 21233214 | 1:1000 | Fulton, S., et al. 2011. Contribution of Kv1.2 voltage-gated potassium channel to D2 autoreceptor regulation of axonal dopamine overflow.. The Journal of Biological Chemistry, 9360-9372. |
| Immunocytochemistry: Rabbit | ||
| PMID | Dilution | Publication |
| 20720182 | not listed | Large, R.J., et al. 2010. Ionic currents in intimal cultured synoviocytes from the rabbit.. American Journal of Physiology-Cell Physiology, C1180-C1194. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 31164806 | 1:100 | Bonetto, G., et al. 2019. Selective axonal expression of the Kv1 channel complex in pre-myelinated GABAergic hippocampal neurons. . Frontiers in Cellular Neuroscience, 222. |
| 23836929 | not listed | Sosanya, N.M., et al. 2013. Degradation of high affinity HuD targets releases Kv1.1 mRNA from miR-129 repression by mTORC1.. The Journal of Biology, 53-69. |
| 21357749 | not listed | Vacher, H., et al. 2011. Cdk-mediated phosphorylation of the Kvβ2 auxiliary subunit regulates Kv1 channel axonal targeting.. The Journal of Biology, 813-24. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 29564531 | 5-20ug/ml | Willis, M., et al. 2018. Shaker-related voltage-gated potassium channels Kv1 in human hippocampus. . Brain Structure and Function, 2663-2671. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 38663634 | not listed | Ramirez-Franco, J, et al. 2024. The downregulation of Kv1 channels in Lgi1-/-mice is accompanied by a profound modification of its interactome and a parallel decrease in Kv2 channels. Neurobiology of Disease, 106513. |
| 34676525 | 1:1000 | Miwa, H., et al. 2021. GAD67-mediated GABA Synthesis and Signaling Impinges on Directing Basket Cell Axonal Projections Toward Purkinje Cells in the Cerebellum.. The Cerebellum, 1-15. |
| 33168717 | not listed | Cho, I.H., et al. 2020. The potassium channel subunit Kvβ1 serves as a major control point for synaptic facilitation. . Proceedings of the National Academy of Sciences, 29937-29947. |
| 28712654 | 1:500 | Favuzzi, E., et al. 2017. Activity-dependent gating of parvalbumin interneuron function by the perineuronal net protein brevican. . Neuron, 639-655. |
| 26269632 | not listed | Smith, K.E., et al. 2015. Phosphoinositide Modulation of Heteromeric Kv1 Channels Adjusts Output of Spiral Ganglion Neurons from Hearing Mice.. Journal of Neuroscience, 11221-11232. |
| 24687876 | 1:250 | King, C.H., et al. 2014. Kv7.2 regulates the function of peripheral sensory neurons.. Journal of Comparative Neurology, 3262-3280. |
| 23090990 | 1:1000 | Nguyen, H.M., et al. 2012. Modulation of voltage-gated K+ channels by the sodium channel β1 subunit.. Proceedings of the National Academy of Science of the United States of America, 18577-18582. |
| 21958379 | not listed | Duflocq, A., et al. 2011. Characterization of the axon initial segment (AIS) of motor neurons and identification of a para-AIS and a juxtapara-AIS, organized by protein 4.1B.. BMC Biology, 1-19. |
| 21676499 | 1:100 | Wykes, R., et al. 2012. Changes in the physiology of CA1 hippocampal pyramidal neurons in preplaque CRND8 mice. . Neurobiology of aging, 1609-1623. |
| 21632949 | 1:500 | Hirtz, J.J., et al. 2011. Cav1.3 calcium channels are required for normal development of the auditory brainstem.. Journal of Neuroscience, 8280-94. |
| 20575068 | 1:100 | Gazula, V.R., et al. 2010. Localization of Kv1.3 channels in presynaptic terminals of brainstem auditory neurons.. Journal of Comparative Neurology, 3205-20. |
| 21233214 | 1:1000 | Fulton, S., et al. 2011. Contribution of Kv1.2 voltage-gated potassium channel to D2 autoreceptor regulation of axonal dopamine overflow.. The Journal of Biological Chemistry, 9360-9372. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 34799447 | 1:100 | Zbili, M., et al. 2021. Homeostatic regulation of axonal Kv1. 1 channels accounts for both synaptic and intrinsic modifications in the hippocampal CA3 circuit. . Proceedings of the National Academy of Sciences, e2110601118. |
| 32345673 | 1:300 | Housley, S.N., et al. 2020. Cancer Exacerbates Chemotherapy-Induced Sensory NeuropathyCancer Exacerbates Chemotherapy-Induced Sensory Neuropathy. . Cancer research, 2940-2955. |
| 24723263 | 1:100 | Dufour, M.A., et al. 2014. Somatodendritic ion channel expression in substantia nigra pars compacta dopaminergic neurons across postnatal development.. Journal of Neuroscience Research, 981-99. |
| 24523557 | 1:100 | Tagoe, T., et al. 2014. Auditory nerve perinodal dysmyelination in noise-induced hearing loss. Journal of Neuroscience, 2684-2688. |
| 23318870 | not listed | Ovsepian, S.V., et al. 2013. A defined heteromeric KV1 channel stabilizes the intrinsic pacemaking and regulates the output of deep cerebellar nuclear neurons to thalamic targets.. The Journal of Physiology, 1771-91. |
| 22473424 | not listed | Huang, C.Y., et al. 2012. Coexpression of high-voltage-activated ion channels Kv3.4 and Cav1.2 in pioneer axons during pathfinding in the developing rat forebrain.. Journal of Comparative Neurology, 3650-3672. |
| 21734302 | 1:200 | Lysakowski, A., et al. 2011. Molecular microdomains in a sensory terminal, the vestibular calyx ending.. Journal of Neuroscience, 10101-10114. |
| Immunohistochemistry: Zebra Finch | ||
| PMID | Dilution | Publication |
| 31937558 | 1:500 | Chen, A.N., et al. 2020. Experience-and sex-dependent intrinsic plasticity in the zebra finch auditory cortex during song memorization. . Journal of Neuroscience, 2047-2055. |
| Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 28712654 | not listed | Favuzzi, E., et al. 2017. Activity-dependent gating of parvalbumin interneuron function by the perineuronal net protein brevican. . Neuron, 639-655. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 29564531 | not listed | Willis, M., et al. 2018. Shaker-related voltage-gated potassium channels Kv1 in human hippocampus. . Brain Structure and Function, 2663-2671. |
| 27872049 | 1:2000 | Nishijima, Y., et al. 2017. Contribution of Kv1. 5 channel to hydrogen peroxide–induced human arteriolar dilation and its modulation by coronary artery disease. . Circulation research, 658-669. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 38663634 | not listed | Ramirez-Franco, J, et al. 2024. The downregulation of Kv1 channels in Lgi1-/-mice is accompanied by a profound modification of its interactome and a parallel decrease in Kv2 channels. Neurobiology of Disease, 106513. |
| 32130108 | 1:1000 | Siems, S.B., et al. 2020. Proteome profile of peripheral myelin in healthy mice and in a neuropathy model.. Elife, e51406. |
| 33157003 | not listed | Kuijpers, M., et al. 2021. Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum. Neuron, 299-313. |
| 32437528 | not listed | Ramberger, M., et al. 2020. Distinctive binding properties of human monoclonal LGI1 autoantibodies determine pathogenic mechanisms. Brain, 1731-1745. |
| 30586380 | not listed | Favaro, P.D., et al. 2018. An opposing function of paralogs in balancing developmental synapse maturation. Plos Biology, e2006838. |
| 29564531 | not listed | Willis, M., et al. 2018. Shaker-related voltage-gated potassium channels Kv1 in human hippocampus. . Brain Structure and Function, 2663-2671. |
| 29476105 | 1:1000 | Nguyen, L.H., et al. 2018. mTOR-dependent alterations of Kv1.1 subunit expression in the neuronal subset-specific Pten knockout mouse model of cortical dysplasia with epilepsy. Scientific Reports, 3568. |
| 28712654 | 1:500 | Favuzzi, E., et al. 2017. Activity-dependent gating of parvalbumin interneuron function by the perineuronal net protein brevican. . Neuron, 639-655. |
| 26269648 | 1:400 | Kole, M.J., et al. 2015. Selective loss of presynaptic potassium channel clusters at the cerebellar basket cell terminal pinceau in adam11 mutants reveals their role in ephaptic control of purkinje cell firing. . Journal of Neuroscience, 11433-11444. |
| 22385959 | not listed | Huang, Y.W., et al. 2012. Dual regulation of miRNA biogenesis generates target specificity in neurotrophin-induced protein synthesis.. Cell, 933-46. |
| 22131424 | 1:500 | Kaphzan, H., et al. 2011. Alterations in intrinsic membrane properties and the axon initial segment in a mouse model of Angelman syndrome.. Journal of Neuroscience, 17637-48. |
| 21943606 | 1:1000 | Sun, W., et al. 2011. DPP6 establishes the A-type K(+) current gradient critical for the regulation of dendritic excitability in CA1 hippocampal neurons.. Neuron, 1102-15. |
| 20575068 | not listed | Gazula, V.R., et al. 2010. Localization of Kv1.3 channels in presynaptic terminals of brainstem auditory neurons.. Journal of Comparative Neurology, 3205-20. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 23792947 | 1:200 | Zhao, X., et al. 2013. A long noncoding RNA contributes to neuropathic pain by silencing Kcna2 in primary afferent neurons.. Nature Neuroscience, 1024-1031. |
| 23536771 | 1:1000 | Brewster, A.L., et al. 2013. Rapamycin reverses status epilepticus-induced memory deficits and dendritic damage.. PLoS One, e57808. |
| 23318870 | 1:10 | Ovsepian, S.V., et al. 2013. A defined heteromeric KV1 channel stabilizes the intrinsic pacemaking and regulates the output of deep cerebellar nuclear neurons to thalamic targets.. The Journal of Physiology, 1771-91. |
| 23066017 | 1:2000 | Yao, J.J., et al. 2012. Neuritin activates insulin receptor pathway to up-regulate Kv4.2-mediated transient outward K+ current in rat cerebellar granule neurons.. The Journal of Biological Chemistry, 41534-41545. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 25707359 | Chen, N., et al. 2015. Interaction proteomics of canonical Caspr2 (CNTNAP2) reveals the presence of two Caspr2 isoforms with overlapping interactomes. . Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 827-833. | |
| 22492029 | Buttermore, E.D., et al. 2012. Pinceau organization in the cerebellum requires distinct functions of neurofascin in Purkinje and basket neurons during postnatal development.. Journal of Neuroscience, 4724-4742. | |
| 22291039 | Ivanovic, A., et al. 2012. The cytoskeletal adapter protein 4.1G organizes the internodes in peripheral myelinated nerves.. The Journal of Biology, 337-44. | |
| 21906755 | Loukaides, P., et al. 2012. Morvan''s syndrome associated with antibodies to multiple components of the voltage-gated potassium channel complex. Journal of the Neurological Sciences, 52-56. | |
| 21220016 | Giacomini, C., et al. 2011. Both Schwann cell and axonal defects cause motor peripheral neuropathy in Ebf2-/- mice.. Neurobiology of Disease, 73-84. | |
| 20392939 | Glasscock, E., et al. 2010. Kv1.1 potassium channel deficiency reveals brain-driven cardiac dysfunction as a candidate mechanism for sudden unexplained death in epilepsy.. Journal of Neuroscience, 5167-5175. | |
| 20133599 | Fukata, Y., et al. 2010. Disruption of LGI1–linked synaptic complex causes abnormal synaptic transmission and epilepsy. . Proceedings of the National Academy of Sciences, 3799-3804. | |
| 20089912 | Ogawa, Y., et al. 2010. ADAM22, a Kv1 channel-interacting protein, recruits membrane-associated guanylate kinases to juxtaparanodes of myelinated axons. . Journal of Neuroscience, 1038-1048. | |
| 19903818 | van der Wijst, J., et al. 2010. Functional analysis of the Kv1.1 N255D mutation associated with autosomal dominant hypomagnesemia.. The Journal of Biological Chemistry, 171-178. | |
| 19818338 | Toyoshima, M., et al. 2009. Deficiency of neural recognition molecule NB-2 affects the development of glutamatergic auditory pathways from the ventral cochlear nucleus to the superior olivary complex in mouse.. Developmental Biology, 192-200. | |
| 19307729 | Glaudemans, B., et al. 2009. A missense mutation in the Kv1.1 voltage-gated potassium channel-encoding gene KCNA1 is linked to human autosomal dominant hypomagnesemia.. Journal of Clinical Investigation, 936-942. | |
| 19091962 | Miller, M.N., et al. 2008. Region-specific spike-frequency acceleration in layer 5 pyramidal neurons mediated by Kv1 subunits.. Journal of Neuroscience, 13716-13726. | |
| 17651419 | Wenzel, H.J., et al. 2007. Structural consequences of Kcna1 gene deletion and transfer in the mouse hippocampus.. Epilepsia, 2023-2046. | |
| 17185748 | Vacher, H., et al. 2007. Regulation of Kv1 channel trafficking by the mamba snake neurotoxin dendrotoxin K.. The FASEB Journal, 906-914. | |
| 17145502 | Gu, C., et al. 2006. The microtubule plus-end tracking protein EB1 is required for Kv1 voltage-gated K+ channel axonal targeting.. Neuron, 803-816. | |
| 18684900 | Pineda, R.H, et al. 2008. Kv1 potassium channel complexes in vivo require Kvβ2 subunits in dorsal spinal neurons. . Journal of neurophysiology, 2125-2136. | |
| 22886464 | Engelhardt, M., et al. 2013. Ankyrin-B structurally defines terminal microdomains of peripheral somatosensory axons.. Brian Structure and Function, 1005-1016. | |