Anti-Lgi1 Antibody (N283/7)

Name: Anti-Lgi1 Antibody
Brand: NeuroMab
SKU: 75-307
Price: 319.0 USD
Availability: InStock

Our Anti-Lgi1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N283/7. It is KO validated, detects human, mouse, and rat Lgi1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunohistochemistry with antigen retrieval via citrate pretreatment.

Adult rat hippocampus immunohistochemistry under standard protocol.

Immunoblot versus whole brain homogenates (homo) from adult rat and WT and Lgi1 KO mouse probed with N283/7 TC supe. Data courtesy of Masaki Fukata (National Institute for Physiological Sciences, Japan).

Immunohistochemistry with antigen retrieval via citrate pretreatment.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-307

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Lgi1

Target Description

Leucine Rich Glioma Inactivated 1 is encoded by the gene LGI1. LGI1 is a member of the secreted leucine-rich repeat (LRR) superfamily. LGI1 may regulate the activity of voltage-gated potassium channels and may be involved in neuronal growth regulation and cell survival. LGI1 is expressed in the brain. Diseases associated with LGI1 include Epilepsy, Familial Temporal Lobe, 1 and Autosomal Dominant Epilepsy with Auditory Features.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N283/7

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Lgi1

Molecular Weight

60 kDa

Antigen

Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse Lgi1 (accession number Q9JIA1) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315876

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with Lgi2, Lgi3 or Lgi4 (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Leucine-rich glioma-inactivated protein 1

UniProt Details

UniProt (Human): O95970
UniProt (Immunogen Species): Q9JIA1

Product Specific References for Applications and Species

ELISA: Human
Immunohistochemistry: Rat
Additional Publications: Unspecified

ELISA: Human
PMID	Dilution	Publication
31900946	1:1000	Kornau, H.C., et al. 2020. Human Cerebrospinal Fluid Monoclonal LGI1 Autoantibodies Increase Neuronal Excitability. Annals of Neurology, 405-418.

Immunohistochemistry: Rat
PMID	Dilution	Publication
28673977	not listed	Seagar, M., et al. 2017. LGI1 tunes intrinsic excitability by regulating the density of axonal Kv1 channels. Proceedings of the National Academy of Sciences, 114(29), pp.7719-7724.. Proceedings of the National Academy of Sciences, 7719-7724.

Additional Publications: Unspecified
PMID	Publication
33097921	Park, G.H., et al. 2020. Activated microglia cause metabolic disruptions in developmental cortical interneurons that persist in interneurons from individuals with schizophrenia. Nature Neuroscience, 1352-1364.

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Anti-Lgi1 Antibody (N283/7)

SKU: 75-307

Product Details

Product Specific References for Applications and Species

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