Anti-LRP4 (Extracellular) Antibody (N207/27)

20 Citations

Our Anti-LRP4 (extracellular) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N207/27. It detects mouse and rat LRP4 (extracellular), and is purified by Protein A chromatography. It is great for use in ICC, WB.

Adult rat brain membrane immunoblot
Adult rat brain membrane immunoblot
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Adult rat brain membrane immunoblot
Representative images of EDL muscles of 3 M, 12 M, and 24 M-old mice, whole mount stained with 555-anti-LRP4 (Cat no 75-221, 1:200, red) and NF/Syn (blue). Arrowheads, non-annulospiral axons had no LRP4 expression. Arrows, LRP4 negative annulospiral endings in aged mice. Scale bar, 10 µm. Image from publication CC-BY-4.0. PMID:36765071
Western blot image of mouse skeletal muscle confirming of expression of Flag-LRP4 (catalog no 75-221, 1:1000). Image from publication CC-BY-4.0. PMID:36765071
Rat ColQ−/− muscle cells were transduced to express ColQ-Myc (+) or not (−). After differentiation, the myotubes were treated (+) or not (−) with 10 nM of neural agrin for 1 h, and MuSK tyrosine phosphorylation was assessed as aforementioned. E, MuSK and LRP4 (cat. 75-221, 1:1000) cs levels of ColQ−/− myotubes re-expressing ColQ-Myc (+) or not (−) and not treated with agrin were analyzed. Image from publication CC-BY-4.0. PMID:37356721

Human, Mouse, Rabbit, Rat
ICC, IHC, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet

SKU: 75-221

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Details

LRP4 (extracellular)
Low-density lipoprotein receptor-related protein 4 or LRP4 is encoded by the gene LRP4. And is a member of the low-density lipoprotein receptor-related protein family. Proteins in this family function in a wide range of physiological processes, including the regulation of lipid metabolism, protection against atherosclerosis, neurodevelopment. LRP4 may be involved in the negative regulation of the canonical Wnt signaling pathway. LRP4 is expressed in bone and several regions of the brain. Mutations in this gene are associated with Cenani-Lenz syndrome.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N207/27
IgG2a
ICC, IHC, WB
Mouse
Lrp4 Kiaa0816
215 kDa
Fusion protein amino acids 26-350 (extracellular N-terminus) of mouse LRP4 (accession number Q8VI56) produced recombinantly in E. Coli
Human, Mouse, Rabbit, Rat
AB_2139030
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:1000
IHC: 1:500
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Low-density lipoprotein receptor-related protein 4 (LRP-4) (LDLR dan)
Q8VI56

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
PMID Dilution Publication
367650711:200Cao, R., et al. 2023. Intrafusal-fiber LRP4 for muscle spindle formation and maintenance in adult and aged animals. Nature Communications, 744.
312684201:1000Zhang, H., et al. 2019. Agrin-Lrp4-Ror2 signaling regulates adult hippocampal neurogenesis in mice.. Elife, e45303.
Patch Clamp Recording: Mouse
PMID Dilution Publication
24462100not listedWang, K., et al. 2014. Distinct Ca2+ sources in dendritic spines of hippocampal CA1 neurons couple to SK and Kv4 channels.. Neuron, 379-387.
Solid Phase Binding Assay: Human
PMID Dilution Publication
34233932not listedYu, Z., et al. 2021. Characterization of LRP4/agrin antibodies from a patient with myasthenia gravis. Neurology, e975-e987.
Western Blot: Mouse
PMID Dilution Publication
367650711:1000Cao, R., et al. 2023. Intrafusal-fiber LRP4 for muscle spindle formation and maintenance in adult and aged animals. Nature Communications, 744.
340905161:500Jing, H., et al. 2021. Synapse-specific Lrp4 mRNA enrichment requires Lrp4/MuSK signaling, muscle activity and Wnt non-canonical pathway. Cell Bioscience, 105.
340639921:1000Hui, T.K., et al. 2021. Ablation of Lrp4 in Schwann cells promotes peripheral nerve regeneration in mice. Biology (Basel), 452.
339876571:1000Hui, T., et al. 2021. Increasing LRP4 diminishes neuromuscular deficits in a mouse model of Duchenne muscular dystrophy. Human Molecular Genetics, 1579-1590.
326876711:1000Chen, A., et al. 2020. APC2 CDH1 negatively regulates agrin signaling by promoting the ubiquitination and proteolytic degradation of DOK7. FASEB, 12009-12023.
324570761:1000Zhang, H., et al. 2020. A role of low-density lipoprotein receptor-related protein 4 (LRP4) in astrocytic Aβ clearance.. Journal of Neuroscience, 5347-5361.
312613761:1000Xiao, Y., et al. 2020. Loss of mitochondrial protein CHCHD10 in skeletal muscle causes neuromuscular junction impairment. Human Molecular Genetics, 1784-1796.
28193701not listedXiong, L., et al. 2017. Osteoblastic Lrp4 promotes osteoclastogenesis by regulating ATP release and adenosine-A2AR signaling.. The Journal of Biology, 761-778.
272945131:1000Sun, X.D., et al. 2016. Lrp4 in astrocytes modulates glutamatergic transmission. Nature Neuroscience, 1010-1018.
254076771:2500Gomez, A.M., et al. 2014. Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4.. Elife, e04287.
253196861:1000Barik, A., et al. 2013. LRP4 is critical for neuromuscular junction maintenance.. Journal of Neuroscience, 13892-13905.
242006891:1000Shen, C., et al. 2013. Antibodies against low-density lipoprotein receptor–related protein 4 induce myasthenia gravis. Journal of Clinical Investigation, 5190-5202.
227942641:1000Wu, H., et al. 2012. Distinct roles of muscle and motoneuron LRP4 in neuromuscular junction formation.. Neuron, 94-107.
Western Blot: Rabbit
PMID Dilution Publication
242006891:1000Shen, C., et al. 2013. Antibodies against low-density lipoprotein receptor–related protein 4 induce myasthenia gravis. Journal of Clinical Investigation, 5190-5202.
Western Blot: Rat
PMID Dilution Publication
373567211:1000Uyen Dao, TM, et al. 2023. The Collagen ColQ Binds to LRP4 and Regulates the Activation of the Muscle-Specific Kinase/LRP4 Receptor Complex by Agrin at the Neuromuscular Junction. The Journal of Biological Chemistry, 104962.
Additional Publications: Unspecified
PMID Publication
38734896Sun, S, et al. 2024. The MuSK agonist antibody protects the neuromuscular junction and extends the lifespan in C9orf72-ALS mice. Molecular Therapy, 0.

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