Our Anti-MARCKS primary antibody from PhosphoSolutions is chicken polyclonal. It detects human MARCKS and is total IgY fraction. It is great for use in WB, IHC, ICC.
Immunolabeling of HeLa cells showing specific labeling of the MARCKs protein (anti-MARCKS antibody, cat. 1107-MARCKS, red, 1:5000) and β-tubulin protein (green). Anti-MARCKS binds MARCKs protein expressed in the plasma membrane and cytoplasm. The blue is Hoechst staining the nuclear DNA.
Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al.,1990) and is concentratedin synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro (Hartwig et al. 1992). Deletion of the MARCKS gene in mice results in embryonic brain defects and death (Stumpo et al.1995). MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser-152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).
Total IgY fraction
Polyclonal
IgY
ICC, IHC, WB
Chicken
MARCKS
80 kDa
Recombinant human MARCKS expressed in and purified from E. coli
Human
Non-Human Primate
AB_2751023
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Total IgY fraction
PBS + 10 mM NaN3.
WB: 1:10000
IHC: 1:500-1:1000
ICC: 1:500-1:5000
Unconjugated
Specific for endogenous levels of the ~80 kDa MARCKS protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
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Ouimet CC, Wang JKT, Walaas SI, Albert KA, Greengard P (1990) Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain. Journal of Neuroscience 10:1683-1698. PMID: 2332803
Hartwig JH, Thelen M, Rosen A, Janmey PA, Nairn AC and Aderem A. (1992) MARCKS is an a ctin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 356: 618-622. PMID: 1560845
Stumpo DJ, Bock CB, Tuttle JS and Blackshear PJ. (1995) MARCKS deficiency in mice leads to abnormal brain development and perinatal death. PNAS 92; 944-948. PMID: 7862670
Verghese GM, Johnson JD, Vasulka C, Haupt DM, Stumpo DJ, Blackshear PJ (1994) Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. Journal of Biological Chemistry 269:9361-9367. PMID: 8132675
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