Our mDia2 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat mDia2 (C-terminal region) and is antigen affinity purified. It is great for use in WB.
Western blot analysis of mDia2 expression in rat PC12 (lane 1), human A431 (lane 2), mouse brain (lane 3), and rabbit spleen fibroblasts (lane 4). The blots were probed with anti-mDia2 (C-terminal region) at 1:500.
Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. The FH1 region contains poly-proline stretches that promote interactions with profilin. The FH2 domain, located C-terminally to the FH1 domain, is highly conserved in formin proteins and possesses actin nucleation and polymerization activities. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. A subgroup of the formins is the diaphanous (Dia) family, which includes mDia1 (Diap1), mDia2 (Diap3), and mDia3 (Diap2). The mDia2 protein has been implicated in cell migration and cytokinesis. This Dia protein can nucleate actin polymerization, as well as bind and stabilize microtubules. mDia2 may also have functions in the nucleus, since it is continually shuttled between the cytoplasm and nucleus
Antigen Affinity Purified
Polyclonal
IgG
WB
Rabbit
DIAPH2
135
mDia2 synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of mouse mDia2 (Diap3). This peptide sequence is highly conserved in rat and human mDia2, and has low homology to other formins.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
Unconjugated
The antibody detects a 135 kDa* protein corresponding to the apparent molecular mass of mDia2 on SDS-PAGE immunoblots of various cells and tissues, including human A431 and HeLa cells, mouse brain tissue and C2C12 cells, rat PC12 cells, and rabbit spleen fibroblasts.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Belin, BJ, et al. 2015. DNA damage induces nuclear actin filament assembly by Formin -2 and Spire-½ that promotes efficient DNA repair.. eLife, e07735.
Qiu, C, et al. 2024. Stub1 promotes degradation of the activated Diaph3: A negative feedback regulatory mechanism of the actin nucleator. The Journal of Biological Chemistry, 107813.
Monzo, P, et al. 2021. Adaptive mechanoproperties mediated by the formin FMN1 characterize glioblastoma fitness for invasion. Developmental cell, 2841-2855.
Egami, Y, et al. 2017. RhoC regulates the actin remodeling required for phagosome formation during FcγR-mediated phagocytosis. Journal of cell science, 4168-4179.
Sandbo, N, et al. 2013. Control of myofibroblast differentiation by microtubule dynamics through a regulated localization of mDia2. The Journal of Biological Chemistry, 15466-15473.
Monzo, P, et al. 2021. Adaptive mechanoproperties mediated by the formin FMN1 characterize glioblastoma fitness for invasion. Developmental cell, 2841-2855.
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