Anti-MeCP2 (Ser-80), Phosphospecific Antibody

4 Citations

Our MeCP2 (Ser-80) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat MeCP2 (Ser-80) and is antigen affinity purified. It is great for use in WB, ICC, IHC.

Western blot of adult mouse brain tissue lysate. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (Ser-80) (lanes 1 & 2) or anti-MeCP2 (C-terminus) (lanes 3 & 4).
Western blot of adult mouse brain tissue lysate. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (Ser-80) (lanes 1 & 2) or anti-MeCP2 (C-terminus) (lanes 3 & 4).
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Western blot of adult mouse brain tissue lysate. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (Ser-80) (lanes 1 & 2) or anti-MeCP2 (C-terminus) (lanes 3 & 4).
Immunocytochemical labeling of MeCP2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with MeCP2 (Ser-80) rabbit polyclonal antibody (MP4601) in the absence (left) or presence (right) of blocking peptide (MX4605). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Human, Mouse, Rat
ICC, IHC, WB
Rabbit

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Data Sheet

SKU: MP4601

Volume: 100 µL
Price:
$340.00
Ships: 1-2 business days

Product Details

MeCP2 (Ser-80)
Methyl-CpG Binding Protein 2 (MeCP2) was identified based on its affinity for methylated cytosines within DNA. As a chromatin-associated multifunctional protein, MeCP2 has been implicated in regulation of transcription and chromatin structure. Mutations of MeCP2 cause Rett syndrome, which results from neuronal dysfunction and impairment in cognitive and motor functions. Regulation of MeCP2 activity may involve phosphorylation at multiple sites. Ser-421 in MeCP2 is phosphorylated in response to neuronal activity, calcium influx, and is dependent on Cam-KII. Alanine mutation of Ser-421 leads to defects in synapse development and activity. Ser-80 in MeCP2 is phosphorylated in HeLa nuclear extracts and neurons. Alanine mutation of Ser-80 attenuates MeCP2 chromatin association and leads to locomotor deficits in transgenic knock-in mice. Thus, phosphorylation of MeCP2 may be important for altering its function during neuronal activity.
Antigen Affinity Purified
Polyclonal
IgG
ICC, IHC, WB
Rabbit
MECP2
75
phospho-MeCP2 (Ser-80) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 80 in mouse MeCP2. This peptide sequence is highly conserved in rat and human MeCP2, and has low homology to other nuclear proteins.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
IHC: 1:100
ICC: 1:250
Unconjugated
The antibody detects a 75 kDa* protein corresponding to the molecular mass of MeCP2 on SDS-PAGE immunoblots of human PC3 cells treated with calyculin A and in mouse brain tissue. These reactivities are not observed after lambda phosphatase treatment.
Phosphorylated
Ser-80
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Methyl-CpG-Binding2
Q9Z2D6

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
286167771:1000Parikh, Z, et al. 2017. Differential Regulation of MeCP2 Phosphorylation by Laminin in Oligodendrocytes. Journal of molecular neuroscience : MN, 309-317.
Immunohistochemistry: Rat
PMID Dilution Publication
274938051:200Muñoz, P, et al. 2016. Inhibition of DNA Methylation Impairs Synaptic Plasticity during an Early Time Window in Rats. Neural Plasticity, 4783836.
Western Blot: Human
PMID Dilution Publication
286167771:1000Parikh, Z, et al. 2017. Differential Regulation of MeCP2 Phosphorylation by Laminin in Oligodendrocytes. Journal of molecular neuroscience : MN, 309-317.
Western Blot: Mouse
PMID Dilution Publication
24636259not listedCheng, T.L., et al. 2014. MeCP2 suppresses nuclear microRNA processing and dendritic growth by regulating the DGCR8/Drosha complex. Developmental Cell, 547-560.
Western Blot: Rat
PMID Dilution Publication
302093411:1000Lee, J., et al. 2018. The acetylation of cyclin-dependent kinase 5 at lysine 33 regulates kinase activity and neurite length in hippocampal neurons. Scientific Reports, 13676.

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