Our Anti-mGluR1/5 (Group I) glutamate receptor mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N75/33. It detects human, mouse, and rat mGluR1/5 (Group I) glutamate receptor, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003). In contrast, the mGluR5 receptor appears to be essential for late phase LTP in area CA1 of the hippocampus (Francesconi et al., 2004).
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N75/33
IgG1
ICC, IHC, IP, WB
Mouse
Grm5 Gprc1e Mglur5
130 kDa (both mGluR1 and mGluR5)
Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b (accession number P31424) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2232873
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.44
Unconjugated
Recognizes mGluR1 and mGluR5
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Granatiero, V., et al. 2019. Nrf2 signaling links ER oxidative protein folding and calcium homeostasis in health and disease. . Life science alliance, e201900563.
Endo, R., et al. 2018. TDP-43 and DISC1 Co-Aggregation Disrupts Dendritic Local Translation and Mental Function in FTLD. Biological Psychiatry, 509-521.
Pretto, D.I., et al. 2014. Reduced excitatory amino acid transporter 1 and metabotropic glutamate receptor 5 expression in the cerebellum of fragile X mental retardation gene 1 premutation carriers with fragile X-associated tremor/ataxia syndrome.. Neurobiology of Aging, 1189-1197.
Endo, R., et al. 2018. TDP-43 and DISC1 Co-Aggregation Disrupts Dendritic Local Translation and Mental Function in FTLD. Biological Psychiatry, 509-521.
Breiderhoff, T., et al. 2013. Sortilin-related receptor SORCS3 is a postsynaptic modulator of synaptic depression and fear extinction.. PLoS One, e75006.
Luo, P., et al. 2013. Downregulation of postsynaptic density-95-interacting regulator of spine morphogenesis reduces glutamate-induced excitotoxicity by differentially regulating glutamate receptors in rat cortical neurons.. FEBS J, 6114-27.
Post, J.I., et al. 2019. Differential Levels and Phosphorylation of Type 1 Inositol 1,4,5-Trisphosphate Receptor in Four Different Murine Models of Huntington Disease. Journal of Huntingtons Disease, 271-289.
Valero, M.L., et al. 2015. TRPC1 and metabotropic glutamate receptor expression in rat auditory midbrain neurons.. Journal of Neuroscience Research, 964-972.
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![Immunoblots on membranes prepared from whole mouse (MBM) and rat (RBM) brain, and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)], and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-116-n75-33-wb-2_{width}x.jpg?v=1626905517)
![Immunoblots on membranes prepared from whole mouse (MBM) and rat (RBM) brain, and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)], and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-116-n75-33-wb-2_1024x.jpg?v=1626905517)
