Anti-Microtubule Associated Protein 2 (MAP2) Antibody

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Our Anti-Microtubule Associated Protein 2 (MAP2) primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, human, mouse, and rat Microtubule Associated Protein 2 (MAP2) and is total IgY fraction. It is great for use in WB, IHC, ICC.

Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti- MAP2(cat. 1100-MAP2, red, 1:10,000), and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti- MAP2(cat. 1100-MAP2, red, 1:10,000), and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
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Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti- MAP2(cat. 1100-MAP2, red, 1:10,000), and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.
Immunofluorescence of a section of rat brain stem showing colabeled with Anti-MAP2 (cat. 1100-MAP2, red, 1:5000) and Anti-FOX3( cat. 583-FOX3 , green, 1:1000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
Immunolabeling of mouse hippocampal cultures labeled with anti-MAP2 (cat. 1100-MAP2, 1:1000, red) and anti-synapsin (cat. 1925-SYNP , 1:1000, green).
Costaining of the primary brain cells isolated from 18-day embryonic rats (E18). Neurons stained with α-MAP2 (green). Copurified astrocytes stained with α-GFAP (red). Image from publication CC-BY-4.0. PMID: 35652638
Immunostaining of rat hippocampus showing specific immunolabeling of MAP2 (cat. 1100-MAP2, red, 1:1000), Tau-1 (green), and nuclear DNA labeled with DAPI (blue). Photo courtesy of the Miriam Zamorano, University of Texas Health Science Center at Houston.
Immunolabeling of mouse hippocampal cultures labeled with Anti-GFAP (cat. 620-GFAP, 1:1000, green) and anti-MAP2 (1100-MAP2, 1:1000, red).
Immunolabeling of mouse cortical cultures labeled with Anti-GFAP (cat. 620-GFAP, 1:1000, green) and anti-MAP2 (1100-MAP2, 1:1000, red).
Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (cat. 1435-NES, red, 1:500) and anti-MAP2 antibody (cat. 1100-MAP2, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
Anti-Microtubule Associated Protein 2 (MAP2) Antibody
Representative images of anti-MAP2 (ca. 1100-MAP2) immunolabeled rat neurons at 5 DIV of T30 treatment. Image from publication CC-BY-4.0. PMID: 37454238
(A-B). Primary hippocampal neurons were stained with MAP2 (cat. 1100-MAP2, 1:1000) as a negative control under basal conditions (CTRL) and with picrotoxin treatment (PTX). Image from publication CC-BY-4.0. PMID: 37654026
Day 14 (D14) RGC-iNs stained with a DAPI, TAU, and MAP2 (cat. 1100-MAP2, 1:1000). Image from publication CC-BY-4.0. PMID: 37773257
Representative 3D view images of paired helical filament (PHF)-tau-treated human brain organoid fractions. The organoids were labeled for MAP2 (Cat no 1100-MAP2, 1:1000, green), NR3A (red), PSD95 (magenta), and DAPI (blue). White arrows show the positions colocalized between PSD95 and NR3A. The dotted circles show the magnified positions. Scale bar = 10 or 3 μm. Image from publication, CC-BY-4.0. PMID: 39396906

Bovine, Human, Mouse, Rat
ICC, IHC, WB
Chicken

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Data Sheet
Trial Size Trial Size

SKU: 1100-MAP2

Volume: 100 µL
Price:
$385.00
Ships: 1-2 business days

Product Details

Microtubule Associated Protein 2 (MAP2)
Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).
Total IgY fraction
Polyclonal
IgY
ICC, IHC, WB
Chicken
MAP2
280 kDa
Recombinant bovine MAP2 protein expressed in and purified from E. Coli.
Bovine, Human, Mouse, Rat
AB_2492141
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Total IgY fraction
PBS + 10 mM NaN3.
WB: 1:20000
IHC: 1:2500-1:10,000
ICC: 1:1000-1:20,000
Unconjugated
Specific for endogenous levels of the ~280 kDa MAP2 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
DKFZp686I2148 antibody, MAP 2 antibody, MAP dendrite specific antibody, MAP-2 antibody, MAP2 antibody, MAP2A antibody, MAP2B antibody, MAP2C antibody, Microtubule associated protein 2 antibody, Microtubule-associated protein 2 antibody, MTAP2_HUMAN antibody
P11137
4133

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
377732571:1000Agarwal, D, et al. 2023. Human retinal ganglion cell neurons generated by synchronous BMP inhibition and transcription factor mediated reprogramming. NPJ Regenerative medicine, 55.
Immunocytochemistry: Mouse
PMID Dilution Publication
39095513not listedLim, W.L., et al. 2024. CREB-regulated transcription during glycogen synthesis in astrocytes. Scientific Reports, 17942.
37694138not listedSoon, HR, et al. 2023. Seizure enhances SUMOylation and zinc-finger transcriptional repression in neuronal nuclei. iScience, 107707.
34247625 1:500 Khamsing, D., et al. 2021. A role for BDNF-and NMDAR-induced lysosomal recruitment of mTORC1 in the regulation of neuronal mTORC1 activity. Molecular Brain, 14(1), pp.1-20.
27762280 1:2000 Allen, M., et al. 2016. Protease induced plasticity: matrix metalloproteinase-1 promotes neurostructural changes through activation of protease activated receptor 1. Scientific Reports, 6.
24784359 1:20,000 Ho, V.M., et al. 2014. GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons. Molecular and Cellular Neuroscience , 61, pp.1-12.
Immunocytochemistry: Rat
PMID Dilution Publication
393080631:500Schneeweis, A., et al. 2024. Mass spectrometry identifies tau C-terminal phosphorylation cluster during neuronal hyperexcitation. Journal of Neurochemistry, .
376540261:1000Abdel-Ghani, M, et al. 2023. Plk2 promotes synaptic destabilization through disruption of N-cadherin adhesion complexes during homeostatic adaptation to hyperexcitation. Journal of Neurochemistry, .
37454238not listedGraur, A, et al. 2023. The Human Acetylcholinesterase C-terminal T30 Peptide Activates Neuronal Growth Through Alpha 7 Nicotinic Acetylcholine Receptors and the mTOR Pathway. Scientific Reports, 11434.
35652638not listedNaumov, A.V., et al. 2022. Restriction Checkpoint Controls Bradyzoite Development in Toxoplasma gondii. Microbiology Spectrum, e0070222.

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