Anti-Microtubule-Associated Protein Antibody

Name: Anti-Microtubule-Associated Protein Antibody
Brand: Aves Labs
SKU: MAP
Price: 455.0 USD
Availability: InStock

33 Citations

Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (P20357) and human (NP_002365) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

MAP-2 immunoreactivity in neurosphere cultures from an e18 mouse brain (1:1000 dilution, green); 2˚ antibody was a fluorescein-labeled goat anti-chicken IgY (Aves Labs Cat No. F-1005, 1:500 dilution). Blue staining is DAPI nuclear stain. (Hoda Ilias, Aves Labs.)

Neonatal mouse brain cortical cultures were stained with Aves Labs MAP2 (green) and GFAP (red, rabbit antibody); Blue is DAPI nuclear stain. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.

Western blot analysis of MAP2 (cat. MAP) in adult mouse cerebral cortex showing significant reduction in the integrated density of MAP2 protein bands normalized with GAPDH. Image from publication CC-BY-4.0. PMID:37416774

Immunostaining of control and Emx1Cre;CasTcKO coronal sections for MZ and SP (white arrows) markers at E15.5. MAP2 (cat. MAP, 1:500), CSPG, Calretinin, and Calbindin show clustering of cells (yellow arrows). CSPG+ cells form a column connecting the SP with the MZ (yellow arrow). Thalamocortical afferents labeled with Calretinin show ectopic bundles extending from the IZ (white arrowheads). DAPI was used as a counterstain (blue). Image from publication CC-BY-4.0. PMID:37540708

MAP2 (cat. MAP) immunostaining of neonatal (P1) mouse cerebral cortex showing a significant decrease in the area ratio of MAP2 fluorescence. Image from publication CC-BY-4.0. PMID:37416774

Representative images (E) and quantification (F) of MAP2 (cat MAP, red) immunofluorescence staining in xenografts dissected from nude mice harbouring 3117-shCtrl or 3117-shDKK4 intraperitoneally. Image from publication CC-BY-4.0. PMID:38519641

MAP-2 immunoreactivity in neurosphere cultures from an e18 mouse brain (1:1000 dilution, green); 2˚ antibody was a fluorescein-labeled goat anti-chicken IgY (Aves Labs Cat No. F-1005, 1:500 dilution). Blue staining is DAPI nuclear stain. (Hoda Ilias, Aves Labs.)

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

Trial Size

SKU: MAP

Volume: 1000 µL

Variant

Price:

$455.00

Quantity:

Product Details

Target

Microtubule-Associated Protein (MAP2)

Target Description

Human MAP-2 is a 199,296 dalton protein (1827 amino acids) expressed in neurons of the PNS and CNS, where it serves as a major component of the neuronal cytoskeleton. MAP-2 contributes to structural integrity and cell shape.

Format

Affinity-Purified IgY

Concentration

200 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

MAP2

Molecular Weight

280 kDa

Antigen

Synthetic peptides

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313549

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the MAP-2 gene product, but are shared between the human (NP_002365, NCBI) and mouse (P20357, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:5000-1:10000
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Quality Control

Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Microtubule-associated protein 2 (MAP-2)

UniProt Details

UniProt (Immunogen Species): P11137

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse | Rat
Immunohistochemistry: Human | Mouse | Rat | Zebra Fish
Western Blot: Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
35625761	1:500	De Kleijn, K.M.A., et al. 2022. Molecular Signature of Neuroinflammation Induced in Cytokine-Stimulated Human Cortical Spheroids. Biomedicines, .
34314701	1:2000	Bowles, K.R., et al. 2021. ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids. Cell, 4547-4563.
28854366	1:1000	Tseng, J.H., et al. 2017. The deacetylase HDAC6 mediates endogenous neuritic tau pathology. Cell Reports, 2169-2183.
20448061	1:200	King, J.E., et al. 2010. Mechanisms of HIV-tat-induced phosphorylation of N-methyl-D-aspartate receptor subunit 2A in human primary neurons: implications for neuroAIDS …. American Journal of Pathology, 2819-2830.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
36510342	1:300	Davis, D., et al. 2022. Localisation of Oestrogen Receptors in Stem Cells and in Stem Cell-Derived Neurons of the Mouse. Journal of Neuroendocrinology, e13220.
35377002	1:500	Marcatti, M., et al. 2022. Aβ/tau oligomer interplay at human synapses supports shifting therapeutic targets for Alzheimer's disease. Cellular and Molecular Life Sciences, 222.
35169866	not listed	Hou, I., et al. 2022. Regulation of dendrite growth by Cdc42 effector protein‐4 in hippocampal neurons in vitro. Molecular Medicine Reports, 128.
35169866	1:10000	Hou, L., et al. 2022. Regulation of dendrite growth by Cdc42 effector protein‑4 in hippocampal neurons in vitro. Molecular medicine reports, 128.
34762981	not listed	Moosecker, S., et al. 2021. Brain Expression, Physiological Regulation and Role in Motivation and Associative Learning of Peroxisome Proliferator-activated Receptor γ. Neuroscience, 91-106.
34453888	1:250	Jiang, L., et al. 2021. Interaction of tau with HNRNPA2B1 and N6-methyladenosine RNA mediates the progression of tauopathy. Molecular Cell, 4209-4227.
33761345	1:500	Wu, K., et al. 2021. Activity- and sleep-dependent regulation of tonic inhibition by Shisa7. Cell Reports, 108899.
32732865	1:1000	Chen, K., et al. 2020. Maf1 regulates dendritic morphogenesis and influences learning and memory. Cell Death & Disease, 606.
32706374	1:1000	Khalaj, A.J., et al. 2020. Deorphanizing FAM19A proteins as pan-neurexin ligands with an unusual biosynthetic binding mechanism. Journal of Cell Biology, .
31551708	1:500	Li, J., et al. 2019. A Conserved Tyrosine Residue in Slitrk3 Carboxyl-Terminus Is Critical for GABAergic Synapse Development. Frontiers in Molecular Neuroscience, 213.
31180354	1:500	Przanowski, P., et al. 2019. A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome. Journal of Visualized Experiments, .
30465259	1:1000	Jiang, L., et al. 2019. TIA1 regulates the generation and response to toxic tau oligomers. Acta Neuropathologica, 259-277.
29107521	1:2000	Li, J., et al. 2017. Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development. Neuron, 808-826.
17348023	1:50	Su, W., et al. 2007. Mice with GFAP‐targeted loss of neurofibromin demonstrate increased axonal MET expression with aging. Glia, 723-733.

Immunocytochemistry: Rat
PMID	Dilution	Publication
30371865	1:200	Ash, P.E.A., et al. 2018. Heavy metal neurotoxicants induce ALS-linked TDP-43 pathology. Toxicological Sciences, 105-115.
28611595	1:2000	Niere, F., et al. 2017. mTORC1 Is a Local, Postsynaptic Voltage Sensor Regulated by Positive and Negative Feedback Pathways. Frontiers in Cellular Neuoroscience, 152.

Immunohistochemistry: Human
PMID	Dilution	Publication
35816404	1:500	Fracassi, A., et al. 2022. TREM2-induced activation of microglia contributes to synaptic integrity in cognitively intact aged individuals with Alzheimer's neuropathology. Brain Pathology, e13108.
28924182	1:200	Shahsavani, M., et al. 2017. An in vitro model of lissencephaly: expanding the role of DCX during neurogenesis. Molecular Psychiatry, 1674-1684.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
38519641	not listed	Li, X, et al. 2024. Colorectal cancer cells secreting DKK4 transform fibroblasts to promote tumour metastasis. Oncogene, 0.
37540708	1:500	Wong, W, et al. 2023. An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold. PLoS Biology, e3002212.
37416774	not listed	Hu, M, et al. 2023. Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice. International journal of biological sciences, 3226-3248.
35169866	1:1000	Hou, L., et al. 2022. Regulation of dendrite growth by Cdc42 effector protein‑4 in hippocampal neurons in vitro. Molecular medicine reports, 128.
35169866	1:500	Hou, I., et al. 2022. Regulation of dendrite growth by Cdc42 effector protein‐4 in hippocampal neurons in vitro. Molecular Medicine Reports, 128.
32732865	1:1000	Chen, K., et al. 2020. Maf1 regulates dendritic morphogenesis and influences learning and memory. Cell Death & Disease, 606.
24193728	1:700	De Koning, A., et al. 2013. Derivation of neural stem cells from an animal model of psychiatric disease. Translational Psychiatry, e323.

Immunohistochemistry: Rat
PMID	Dilution	Publication
32848574	not listed	Wang, C., et al. 2020. Delayed (21 Days) Post Stroke Treatment With RPh201, a Botany-Derived Compound, Improves Neurological Functional Recovery in a Rat Model of Embolic …. Frontiers in Neuroscience, 813.
28924864	1:1000	Zhang, S., et al. 2017. A Nociceptive Role for Integrin Signaling in Pain After Mechanical Injury to the Spinal Facet Capsular Ligament. Annals of Biomedical Engineering, 2813-2825.

Immunohistochemistry: Zebrafish
PMID	Dilution	Publication
37416774	not listed	Hu, M, et al. 2023. Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice. International journal of biological sciences, 3226-3248.

Western Blot: Mouse
PMID	Dilution	Publication
37416774	not listed	Hu, M, et al. 2023. Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice. International journal of biological sciences, 3226-3248.

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Anti-Microtubule-Associated Protein Antibody

SKU: MAP

Product Details

Product Specific References for Applications and Species

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