Our Anti-Motor Neuron and Pancreas Homeobox Protein 1 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat MNX1, and is affinity-purified IgY. It is great for use in ICC, IHC, WB.
Immunofluorescent staining of MCF7 cells using 1 µg/mL chicken anti-HB9 (MNX1) antibody (green). Actin filaments were stained using Phalloidin (red). The cells were mounted with ICT's Fluoroshield mounting medium (Cat no. AR-6500). Anti-HB9/MNX1 specifically stains the nucleus of MCF7 cells.
The MNX1/HB9 protein, encoded by the MNX1 gene (also known as HB9), is a transcription factor belonging to the homeobox gene family, which plays an essential role in regulating gene expression during embryonic development. MNX1/HB9 is particularly important in the spinal cord, where it drives the differentiation of motor neurons. Disruptions in MNX1 expression have been associated with developmental disorders and diseases such as spinal muscular atrophy and Currarino syndrome (Han et al 2020). MNX1 also plays a role in certain forms of diabetes, affecting the development of insulin-producing beta cells in the pancreas (Aly et al. 2023). Due to its influence in multiple body systems, understanding MNX1/HB9 function has potential therapeutic implications for both neurodegenerative and metabolic diseases.
Affinity-Purified IgY
200 µg/mL
Polyclonal
IgY
ICC, IHC, WB
Chicken
MNX1
40 kDa
Recombinant protein encompassing the C-terminal ~150 amino acids of human HB9/MNX1
Human, Mouse, Rat
AB_3665366
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
WB: 1:200-1:1000
ICC: 1:50-1:200
Unconjugated
No cross-reactivity reported
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from receipt of product
Ice packs
HB9, HOXHB9, SCRA1, HLXB9, Motor Neuron And Pancreas Homeobox Protein 1, Homeobox Protein HB9
