Anti-Nav1.8 Na+ Channel Antibody (N134/12)

33 Citations

Our Anti-Nav1.8 Na+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N134/12. It is KO validated, detects human, mouse, and rat Nav1.8 Na+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunostaining of a 7 µm thick DRG neuron section from a 3 month old rat labeling NaV1.8 (green, 1:2000, Cat. 75-166), IB4 (red), and nuclei is stained with DAPI (blue). The image is kindly provided by Cristian Acosta, Universidad Nacional de Cuyo, Mendoza, Argentina.
Immunostaining of a 7 µm thick DRG neuron section from a 3 month old rat labeling NaV1.8 (green, 1:2000, Cat. 75-166), IB4 (red), and nuclei is stained with DAPI (blue). The image is kindly provided by Cristian Acosta, Universidad Nacional de Cuyo, Mendoza, Argentina.
Roll over image to zoom in Click on image to zoom
Immunostaining of a 7 µm thick DRG neuron section from a 3 month old rat labeling NaV1.8 (green, 1:2000, Cat. 75-166), IB4 (red), and nuclei is stained with DAPI (blue). The image is kindly provided by Cristian Acosta, Universidad Nacional de Cuyo, Mendoza, Argentina.
Stable/transfected cell immunoblot: extracts of HEK cells stably expressing Nav1.7 and COS cells transiently transfected with GFP-tagged Nav1.8 or untagged Kv2.1 plasmids and probed with N134/12 TC supe.
Immunofluorescence staining of cultured dorsal root ganglia neurons from Nav1.8 WT and KO mice with N134/12 TC supe. Image courtesy of Dr. Joel Black, Dr. Sulayman Dib-Hajj and Dr. Steve Waxman, Yale University.
Immunofluorescence staining of rat dorsal root ganglia cryosections. N134/12 TC supe = green, neurofilament = red. Image courtesy of Dr. Joel Black, Dr. Sulayman Dib-Hajj and Dr. Steve Waxman, Yale University.

Human, Mouse, Rat
ICC, IHC, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet
KO Validated KO Validated

SKU: 75-166

Volume: 100 µL
Price:
$385.00
Ships: 1-2 business days

Product Details

Nav1.8 Na+ channel
NAV1.8 is a member of the voltage-gated sodium channel family, which function in producing and propagating action potentials. Sodium channels are composed of a large alpha subunit that may be associated with one or more smaller beta subunits. Nav 1.8 is an integral membrane glycoprotein that is responsible for the initial rising phase of action in most excitable cells. Nav 1.8 is expressed in neurons in the dorsal root ganglion (DRG). and appears to have a role in pain pathways.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N134/12
IgG2a
ICC, IHC, WB
Mouse
Scn10a Sns
220 kDa
Fusion protein amino acids 1724-1956 (cytoplasmic C-terminus) of rat Nav1.8 (accession number Q62968) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2183861
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.93
WB: 1:1000
IHC: 1:250
ICC: 1:250
Unconjugated
Does not react with Nav1.7
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Sodium channel protein type 10 subunit alpha (Peripheral nerve sodium channel 3) (PN3) (Sensory neuron sodium channel) (Sodium channel protein type X subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.8)
Q62968

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
391422811:1000Kantarci, H., et al. 2024. Schwann cell-secreted PGE2 promotes sensory neuron excitability during development. Cell, 4690-4712.e30.
Immunocytochemistry: Rat
PMID Dilution Publication
344319811:500Isensee, J., et al. . Depolarization induces nociceptor sensitization by CaV1.2-mediated PKA-II activation. Journal of Cell Biology, e202002083.
315014501:200Vysokov, N., et al. . The role of Na V channels in synaptic transmission after axotomy in a microfluidic culture platform. Scientific Reports, 12915.
244533371:2000Acosta, C., et al. . TREK2 expressed selectively in IB4-binding C-fiber nociceptors hyperpolarizes their membrane potentials and limits spontaneous pain.. Journal of Neuroscience, 1494-509.
242783111:500Tsantoulas, C., et al. . Probing functional properties of nociceptive axons using a microfluidic culture system.. PLoS One, e80722.
211185381:100Persson, A.K., et al. . Sodium-calcium exchanger and multiple sodium channel isoforms in intra-epidermal nerve terminals. . Molecular Pain, 1744-8069.
Immunohistochemistry: Human
PMID Dilution Publication
22794211Kim, Y.S., et al. . Expression of transient receptor potential ankyrin 1 in human dental pulp. Journal of Endodontics, 1087-92.
Immunohistochemistry: Mouse
PMID Dilution Publication
39582088not listedLenert, M.E., et al. 2024. Sensory neuron LKB1 mediates ovarian and reproductive function. Scientific Reports, 29109.
391422811:200Kantarci, H., et al. 2024. Schwann cell-secreted PGE2 promotes sensory neuron excitability during development. Cell, 4690-4712.e30.
349670031:500Mason, B.N., et al. . Prolactin signaling modulates stress-induced behavioral responses in a preclinical mouse model of migraine. Headache, 11-25.
32077526Song, G.J., et al. . Loss-of-function of EBP50 is a new cause of hereditary peripheral neuropathy: EBP50 functions in peripheral nerve system. Glia, 1794-809.
312901571:500Lainez, S., et al. . HCN3 ion channels: roles in sensory neuronal excitability and pain. The Journal of Physiology, 4661-75.
296975311:1000Tsantoulas, C., et al. . Mice lacking Kcns1 in peripheral neurons show increased basal and neuropathic pain sensitivity. Pain, 1641-51.
259954841:10Lu, V.B., et al. . A 3.7 kb fragment of the mouse Scn10a gene promoter directs neural crest but not placodal lineage EGFP expression in a transgenic animal.. Journal of Neuroscience, 8021-34.
256008721:250François, A., et al. . The Low-Threshold Calcium Channel Cav3.2 Determines Low-Threshold Mechanoreceptor Function.. Cell Report, 370-82.
249038311:200Montersino, A., et al. . Tetrodotoxin-resistant voltage-gated sodium channel Nav 1.8 constitutively interacts with ankyrin G.. Journal of Neurochemistry, 33-41.
238849571:500Lippoldt, E.K., et al. . Artemin, a glial cell line-derived neurotrophic factor family member, induces TRPM8-dependent cold pain.. Journal of Neuroscience, 12543-52.
234079431:500Knowlton, W.M., et al. . A sensory-labeled line for cold: TRPM8-expressing sensory neurons define the cellular basis for cold, cold pain, and cooling-mediated analgesia. Journal of Neuroscience, 2837-48.
Immunohistochemistry: Rat
PMID Dilution Publication
384835561:2000Messina, DN, et al. 2024. Complex alterations in inflammatory pain and analgesic sensitivity in young and ageing female rats: involvement of ASIC3 and Nav1.8 in primary sensory neurons. Inflammation Research, 669-691.
364785411:2000Messina, D.N., et al. 2022. Age-dependent and Modality-specific Changes in the Phenotypic Markers Nav1.8, ASIC3, P2X3 and TRPM8 in Male Rat Primary Sensory Neurons During Healthy Aging. Biogerontology, 111-136.
1:1000Tsantoulas, C., et al. 2022. HCN2 Ion Channels Drive Pain in Rodent Models of Migraine. The Journal of Neuroscience, 7513-7529.
25609640not listedRitter, D.M., et al. . Dysregulation of Kv3.4 channels in dorsal root ganglia following spinal cord injury.. Journal of Neuroscience, 1260-73.
236424080.25 µg/mLLiang, L., et al. . Protein kinase B/Akt is required for complete Freund''s adjuvant-induced upregulation of Nav1.7 and Nav1.8 in primary sensory neurons.. The Journal of Pain, 638-47.
219656681:500Ruangsri, S., et al. . Relationship of axonal voltage-gated sodium channel 1.8 (NaV1.8) mRNA accumulation to sciatic nerve injury-induced painful neuropathy in rats. Journal of Biological Chemistry, 39836-47.
211185381:100Persson, A.K., et al. . Sodium-calcium exchanger and multiple sodium channel isoforms in intra-epidermal nerve terminals. . Molecular Pain, 1744-8069.
MicroArray: Mouse
PMID Dilution Publication
349310701:500Yang, N.J., et al. . Anthrax toxins regulate pain signaling and can deliver molecular cargoes into ANTXR2+ DRG sensory neurons. Nature Neuroscience, 168-79.
Western Blot: Mouse
PMID Dilution Publication
336362251:1000Sun, L., et al. . MicroRNA-96 is required to prevent allodynia by repressing voltage-gated sodium channels in spinal cord. Progress in Neurobiology, 102024.
285152301:1000Isensee, J., et al. . Crosstalk from cAMP to ERK1/2 emerges during postnatal maturation of nociceptive neurons and is maintained during aging. Journal of Cell Science, 2134-46.
Western Blot: Rat
PMID Dilution Publication
237929471:1000Zhao, X., et al. 2013. A long noncoding RNA contributes to neuropathic pain by silencing Kcna2 in primary afferent neurons.. Nature Neuroscience, 1024-1031.
236424081:1000Liang, L., et al. . Protein kinase B/Akt is required for complete Freund''s adjuvant-induced upregulation of Nav1.7 and Nav1.8 in primary sensory neurons.. The Journal of Pain, 638-47.
20026672not listedHoffman, E.M., et al. . Fixative Composition Alters Distributions of Immunoreactivity for Glutaminase and Two Markers of Nociceptive Neurons, Nav1.8 and TRPV1, in the Rat Dorsal Root Ganglion. Journal of Histochemistry and Cytochemistry, 329-44.
Additional Publications: Unspecified
PMID Publication
21729793Spofford, C.M., et al. . Evaluation of leukemia inhibitory factor (LIF) in a rat model of postoperative pain. The Journal of Pain, 819-32.

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