Anti-NFH (Neurofilament Heavy Chain) Antibody

83 Citations

NFH protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion” (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFH protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. These antibodies have been found to cross-react with NFH protein from a variety of species, including human, mouse and rat.

Cortical neuron from a neo-natal mouse brain culture immunocytochemically stained for NF-H immunoreactivity (1:5000 dilution). Secondary antibody (fluorescein-labeled goat anti-chicken IgY, (1:1000 dilution)
Confocal micrographs of DRG cryosections from WT mouse double-labeled with antibodies against caldendrin (green) and NF200 (Cat no. NFH, 1:1000, purple) or peripherin (Cat no. PER, 1:1000, purple). Arrows and arrowheads indicate cells in which caldendrin is or is not, respectively, co-localized with NF200 or peripherin. Results are representative of at least 3 independent experiments. Scale bar, 100 µm. Image from publication CC-BY-4.0. PMID:36788334
Confocal micrographs of caldendrin KO cultures infected with AAV-mRuby (top) or AAV-mRuby-caldendrin (bottom) showing immunolabeling for NF200 (Cat no. NFH, 1:1000, left) and mRuby (right). NF200-positive DRGNs with and without mRuby fluorescence are indicated by arrows and arrowheads, respectively. Scale bar, 100 µm. Image from publication CC-BY-4.0. PMID:36788334
Representative images of sections of a rat G muscle 7, 14, 21, and 60 dpi, immunostained against NFH (cat. NFH, 1:200; green), α-btx (red), and VAChT (blue), respectively, to label axons, neuromuscular junctions, and the synaptic vesicle-filled motor end plate. Larger numbers of reinnervated NMJs were observed with increased time after injury, with almost complete innervation at 60 dpi. The nicotinic receptor field expands after early denervation. Image from publication CC-BY-4.0. PMID: 36759186
Example images of back hairy skin wholemount staining of NFH (cat. NFH, 1:500) and S100B in control and mutant mice. Overall innervation and lanceolate ending morphologyImage from publication CC-BY-4.0. PMID:37607547
Forelimb pedal pad sections of control Npy2r-GFP glabrous skin (top) and Prx1Cre; Lmx1bfl/fl; Npy2r-GFP double glabrous mutant, ectopic glabrous skin (bottom). The sections are stained using S100, GFP, and NFH (cat. NFH, 1:500). Red arrows point to the GFP- axon (presumably Ret+).Image from publication CC-BY-4.0. PMID:37607547
Sections of P21 control and double glabrous forepaws with NFH (cat. NFH, 1:500), S100, and DAPI. Control ventral and dorsal skin regions (top two rows) have Meissner corpuscles on the ventral side and lanceolate endings on the dorsal side. Double glabrous mutant ventral and dorsal skin (bottom two rows) is predominantly glabrous, and these mutants contain Meissner corpuscles on both the ventral and dorsal sides of the paw. Image from publication CC-BY-4.0. PMID:37607547
(A and B) Example images of TrkB+ endings (TrkBGFP or TrkBCreER; AvilFlp; RosaLSL-FSF-Tdtomato) in paw glabrous skin (A) and paw hairy skin (B) at E15.5, P5, and P50. The sections were stained with S100, NFH (cat, NFH, 1:500) and GFP or tdTomato. The white dotted lines represent the dermal-epidermal junction in glabrous skin and the hair follicle outline in hairy skin. Image from publication CC-BY-4.0. PMID:37607547
Representative images of mouse spinal cord throughout the rostral-caudal extent of injury 42 days after spinal cord injury (SCI). Sections are stained for myelin (blue, erichrome cyanine) and axons (brown, neurofilament, cat. NFH, 1:1500). Frank lesion areas are highlighted in red. Spared tissue is evident by both blue and brown staining. Image from publication CC-BY-4.0. PMID:37350129
NFH (axonal tracts, cat. NFH) and MBP (myelin) immunostaining of the corpus callosum sections. Compared with WT (C57BL/6 wildtype) and mTrappc9+/+, the corpus callosum (highlighted with white dotted lines) of mTrappc9m/m mice was significantly thinner. Image from publication CC-BY-4.0. PMID:37416774
Western blot analysis of MBP and NFH protein expression in cerebral cortex normalized with GAPDH. Both showed a significant decrease in mTrappc9 m/m mice. Image from publication CC-BY-4.0. PMID:37416774
Cortical neuron from a neo-natal mouse brain culture immunocytochemically stained for NF-H immunoreactivity (1:5000 dilution). Secondary antibody (fluorescein-labeled goat anti-chicken IgY, (1:1000 dilution)
Cortical neuron from a neo-natal mouse brain culture immunocytochemically stained for NF-H immunoreactivity (1:5000 dilution). Secondary antibody (fluorescein-labeled goat anti-chicken IgY, (1:1000 dilution)
Confocal micrographs of DRG cryosections from WT mouse double-labeled with antibodies against caldendrin (green) and NF200 (Cat no. NFH, 1:1000, purple) or peripherin (Cat no. PER, 1:1000, purple). Arrows and arrowheads indicate cells in which caldendrin is or is not, respectively, co-localized with NF200 or peripherin. Results are representative of at least 3 independent experiments. Scale bar, 100 µm. Image from publication CC-BY-4.0. PMID:36788334
Confocal micrographs of caldendrin KO cultures infected with AAV-mRuby (top) or AAV-mRuby-caldendrin (bottom) showing immunolabeling for NF200 (Cat no. NFH, 1:1000, left) and mRuby (right). NF200-positive DRGNs with and without mRuby fluorescence are indicated by arrows and arrowheads, respectively. Scale bar, 100 µm. Image from publication CC-BY-4.0. PMID:36788334
Representative images of sections of a rat G muscle 7, 14, 21, and 60 dpi, immunostained against NFH (cat. NFH, 1:200; green), α-btx (red), and VAChT (blue), respectively, to label axons, neuromuscular junctions, and the synaptic vesicle-filled motor end plate. Larger numbers of reinnervated NMJs were observed with increased time after injury, with almost complete innervation at 60 dpi. The nicotinic receptor field expands after early denervation. Image from publication CC-BY-4.0. PMID: 36759186
Example images of back hairy skin wholemount staining of NFH (cat. NFH, 1:500) and S100B in control and mutant mice. Overall innervation and lanceolate ending morphologyImage from publication CC-BY-4.0. PMID:37607547
Forelimb pedal pad sections of control Npy2r-GFP glabrous skin (top) and Prx1Cre; Lmx1bfl/fl; Npy2r-GFP double glabrous mutant, ectopic glabrous skin (bottom). The sections are stained using S100, GFP, and NFH (cat. NFH, 1:500). Red arrows point to the GFP- axon (presumably Ret+).Image from publication CC-BY-4.0. PMID:37607547
Sections of P21 control and double glabrous forepaws with NFH (cat. NFH, 1:500), S100, and DAPI. Control ventral and dorsal skin regions (top two rows) have Meissner corpuscles on the ventral side and lanceolate endings on the dorsal side. Double glabrous mutant ventral and dorsal skin (bottom two rows) is predominantly glabrous, and these mutants contain Meissner corpuscles on both the ventral and dorsal sides of the paw. Image from publication CC-BY-4.0. PMID:37607547
(A and B) Example images of TrkB+ endings (TrkBGFP or TrkBCreER; AvilFlp; RosaLSL-FSF-Tdtomato) in paw glabrous skin (A) and paw hairy skin (B) at E15.5, P5, and P50. The sections were stained with S100, NFH (cat, NFH, 1:500) and GFP or tdTomato. The white dotted lines represent the dermal-epidermal junction in glabrous skin and the hair follicle outline in hairy skin. Image from publication CC-BY-4.0. PMID:37607547
Representative images of mouse spinal cord throughout the rostral-caudal extent of injury 42 days after spinal cord injury (SCI). Sections are stained for myelin (blue, erichrome cyanine) and axons (brown, neurofilament, cat. NFH, 1:1500). Frank lesion areas are highlighted in red. Spared tissue is evident by both blue and brown staining. Image from publication CC-BY-4.0. PMID:37350129
NFH (axonal tracts, cat. NFH) and MBP (myelin) immunostaining of the corpus callosum sections. Compared with WT (C57BL/6 wildtype) and mTrappc9+/+, the corpus callosum (highlighted with white dotted lines) of mTrappc9m/m mice was significantly thinner. Image from publication CC-BY-4.0. PMID:37416774
Western blot analysis of MBP and NFH protein expression in cerebral cortex normalized with GAPDH. Both showed a significant decrease in mTrappc9 m/m mice. Image from publication CC-BY-4.0. PMID:37416774

Human, Mouse, Rat
ICC, IHC, WB
Chicken

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Data Sheet

SKU: NFH

Volume: 200 µL
1-2 business days
Price:
$285.00

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
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Immunocytochemistry: Rat
PMID Dilution Publication
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206681951:1000Alexander, J.K., et al. 2010. Ric-3 promotes α7 nicotinic receptor assembly and trafficking through the ER subcompartment of dendrites. Journal of Neuroscience, 10112-10126.
Immunohistochemistry: Mouse
PMID Dilution Publication
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337377071:1000Kopper, T.J., et al. 2021. The effects of myelin on macrophage activation are phenotypic specific via cPLA2 in the context of spinal cord injury inflammation. Scientific Reports, 6341.
334225521:2500Stewartac, A.N., et al. 2021. Mitochondria exert age-divergent effects on recovery from spinal cord injury. Experimental Neurology, 113597.
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330518531:250Chen, J.Y., et al. 2021. Lentiviral Interleukin-10 Gene Therapy Preserves Fine Motor Circuitry and Function After a Cervical Spinal Cord Injury in Male and Female Mice. Neurotherapeutics, 503-514.
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326751741:1000Wang, Z., et al. 2020. The Purinergic Receptor P2rx3 is Required for Spiral Ganglion Neuron Branch Refinement during Development. eNeuro, .
325545681:500Neubarth, N.L., et al. 2020. Meissner Corpuscles and Their Spatially Intermingled Afferents Underlie Gentle Touch Perception. Science, eabb2751.
323800321:1000Orr, B.O., et al. 2020. Presynaptic homeostasis opposes disease progression in mouse models of ALS-like degeneration: evidence for homeostatic neuroprotection. Neuron, 95-111.
319947261:2500Brooks, P.M., et al. 2020. Pou3f4‐expressing otic mesenchyme cells promote spiral ganglion neuron survival in the postnatal mouse cochlea. Journal of Comparative Neurology, 1967-1985.
319646861:500Hablitz, L.M., et al. 2020. Cannabinoid signaling recruits astrocytes to modulate presynaptic function in the suprachiasmatic nucleus. eNeuro, .
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313099271:400Stavoe, A.K.H, et al. 2019. Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons.. Elife, e44219.
312487281:1000Zheng, Y., et al. 2019. Deep Sequencing of Somatosensory Neurons Reveals Molecular Determinants of Intrinsic Physiological Properties.. Neuron, 598-616.
312091731:2000Jung, J.S., et al. 2019. Semaphorin-5B Controls Spiral Ganglion Neuron Branch Refinement during Development.. Journal of Neuroscience, 6425-6438.
308660421:400Goel, M., et al. 2019. Differential expression and sub-cellular localization of Copines in mouse retina. Journal of Comparative Neurology, 2245-2262.
307267281:500-1:1000Zhang, M., et al. 2019. Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice. Cell Reports, 1419-1431.
306867321:500Green, D.P., et al. 2019. A Mast-Cell-Specific Receptor Mediates Neurogenic Inflammation and Pain. Neuron, 412-420.
304783401:10000Colina, C., et al. 2018. Selective tracking of FFAR3-expressing neurons supports receptor coupling to N-type calcium channels in mouse sympathetic neurons. Scientific reports, 17379.
304530221:500Zhang, B., et al. 2018. Reducing age-dependent monocyte-derived macrophage activation contributes to the therapeutic efficacy of NADPH oxidase inhibition in spinal cord injury. Brain, Behavior, and Immunity, 139-150.
304061831:100Micheva, K.D., et al. 2018. Distinctive Structural and Molecular Features of Myelinated Inhibitory Axons in Human Neocortex.. eNeuro, .
298745801:1000Kridsada, K., et al. 2018. Roof Plate-Derived Dorsal Midline Radial Glial-like Cells Promote Longitudinal Ascending Axon Growth of Rapidly Adapting Mechanoreceptors during Development. Cell Reports, 2928-2941.
295365901:5000Harley, R.J., et al. 2018. Neuronal Cell Adhesion Molecule (NrCAM) is Expressed by Sensory Cells in the Cochlea and is Necessary for Proper Cochlear Innervation and Sensory Domain Patterning During Development. Developmental Dynamics, 934-950.
294764081:200Zukor, K., et al. 2018. Zika virus-induced acute myelitis and motor deficits in adult interferon αβ/γ receptor knockout mice. Journal of NeuroVirology, 273-290.
293292111:200Morrey, J.D., et al. 2018. Causation of Acute Flaccid Paralysis by Myelitis and Myositis in Enterovirus-D68 Infected Mice Deficient in Interferon αβ/γ Receptor Deficient Mice. Viruses, 33.
292793101:1000Hesp, Z.C., et al. 2018. Proliferating NG2-Cell-Dependent Angiogenesis and Scar Formation Alter Axon Growth and Functional Recovery After Spinal Cord Injury in Mice. The Journal of Neuroscience, 1366-1382.
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286478561:500Shih, Y., et al. 2017. Glial and Neuronal Protein Tyrosine Phosphatase Alpha (PTPα) Regulate Oligodendrocyte Differentiation and Myelination. Journal Molecular Neuroscience, 329-343.
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282649781:200Freria, C.M., et al. 2017. Deletion of the fractalkine receptor, CX3CR1, improves endogenous repair, axon sprouting and synaptogenesis after spinal cord injury in mice. The Journal of Neuroscience, 3568-3587.
281628071:1000François, A., et al. 2017. A Brainstem-Spinal Cord Inhibitory Circuit for Mechanical Pain Modulation by GABA and Enkephalins. Neuron, 822-839.
280418521:1000Abraira, V.E., et al. 2017. The Cellular and Synaptic Architecture of the Mechanosensory Dorsal Horn. Cell, 295-310.
281687031:1000Geary, M.B., et al. 2016. Erythropoietin accelerates functional recovery after moderate sciatic nerve crush injury. Muscle & Nerve, 143-151.
280024121:1000Nonomura, K., et al. 2016. Piezo2 senses airway stretch and mediates lung inflation-induced apnoea. Nature, 176-181.
27810921not listedKigerl, K.A., et al. 2016. Gut dysbiosis impairs recovery after spinal cord injury. Journal of Experimental Medicine, 2603-2620.
277079701:500Fleming, M.S., et al. 2016. A RET-ER81-NRG1 Signaling Pathway Drives the Development of Pacinian Corpuscles. The Journal of Neuroscience, 10337-10355.
275963351:500Zhang, B., et al. 2016. Age increases reactive oxygen species production in macrophages and potentiates oxidative damage after spinal cord injury. Neurobiology of Aging, 157-167.
263569881:400Sajgo, S., et al. 2015. Dynamic expression of transcription factor Brn3b during mouse cranial nerve development. Journal of Comparative Neurology, 1033-1061.
254512511:5000Yamada, H., et al. 2014. Transplantation of mature adipocyte-derived dedifferentiated fat cells promotes locomotor functional recovery by remyelination and glial scar reduction after spinal cord injury in mice. Biochemical and Biophysical Research Communications, 341-346.
225756001:2000Alexander, J.K., et al. 2012. Macrophage migration inhibitory factor (MIF) is essential for inflammatory and neuropathic pain and enhances pain in response to stress. Experimental Neurology, 351-362.
197705131ug/mlAnkeny, D.P., et al. 2009. B cells produce pathogenic antibodies and impair recovery after spinal cord injury in mice. Journal of Clinical Investigation, 2990-2999.
Immunohistochemistry: Rat
PMID Dilution Publication
367591861:200Arbat-Plana, A., et al. 2023. Massive Loss of Proprioceptive Ia Synapses in Rat Spinal Motoneurons after Nerve Crush Injuries in the Postnatal Period. eNeuro, .
334686721:200Meregalli, C., et al. 2021. Pathogenic role of delta 2 tubulin in bortezomib-induced peripheral neuropathy. PNAS, .
293807491:2000Cornelison, R.C., et al. 2018. Injectable hydrogels of optimized acellular nerve for injection in the injured spinal cord. Biomedical Materials, 34110.
263909121:500Moldavan, M., et al. 2015. Localization and expression of GABA transporters in the suprachiasmatic nucleus. European Journal of Neuroscience, 3018-3032.
249990281:200Popovich, P.G., et al. 2014. Independent evaluation of the anatomical and behavioral effects of Taxol in rat models of spinal cord injury. Experimental Neurology, 97-108.
Western Blot: Mouse
PMID Dilution Publication
37416774not listedHu, M, et al. 2023. Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice. International journal of biological sciences, 3226-3248.

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