Anti-NGL-2/LRRC4 Antibody (N50/36)
Our Anti-NGL-2/LRRC4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N50/36. It is KO validated, detects mouse and rat NGL-2/LRRC4, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.


Adult mouse brain immunoblot: extracts of brains from wild-type (1), Netrin-G2 knockout (2) and NGL-2 knockout (3) mice were probed with Netrin-G2 antibody (left) or N50/36 (right). Reproduced with permission from 2007 Zhang et al Genes Brain Behav.
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Mouse, Rat
ICC, IHC, WB
Mouse

SKU: 75-084
Product Details
NGL-2/LRRC4
Leucine Rich Repeat Containing 4 is encoded by the gene LRRC4. LRRC4 is a synaptic adhesion protein that regulates the formation of excitatory synapses through the recruitment of pre and postsynaptic proteins. LRRC4 organizes the lamina pathway-specific differentiation of dendrites, and plays an important role in auditory synaptic responses. LRRC4 is expressed in the brain. LRRC4 is significantly downregulated in primary brain tumors.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N50/36
IgG1
ICC, IHC, WB
Mouse
Lrrc4
100 kDa
Fusion protein amino acids 550-652 of mouse NGL-2 (accession number AAG60620) produced recombinantly in E. Coli
Mouse, Rat
AB_2137614
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.14
IHC: 1:100
Unconjugated
No cross-reactivity against NGL-1 or NGL-3
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Leucine-rich repeat-containing protein 4 (Brain tumor-associated protein MBAG1) (Netrin-G2 ligand) (NGL-2)
Product Specific References for Applications and Species
Immunohistochemistry: Mouse | ||
PMID | Dilution | Publication |
29553369 | 1:100 | Soto, F., et al. 2018. Synapse maintenance and restoration in the retina by NGL2. eLife, e30388. |
23864682 | 1:100 | Soto, F., et al. 2013. NGL-2 regulates pathway-specific neurite growth and lamination, synapse formation, and signal transmission in the retina. The Journal of neuroscience : the official journal of the Society for Neuroscience, 11949-11959. |
Western Blot: Mouse | ||
PMID | Dilution | Publication |
23177961 | not listed | DeNardo, L., et al. 2012. NGL-2 regulates input-specific synapse development in CA1 pyramidal neurons. Neuron, 762-775. |
17973922 | not listed | Zhang, W., et al. 2008. Netrin-G2 and netrin-G2 ligand are both required for normal auditory responsiveness. Genes, brain, and behavior, 385-392. |