Anti-NMDA NR2A Subunit Antibody

22 Citations

Our Anti-NMDA NR2A Subunit rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, rabbit, and rat NMDA NR2A Subunit and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, IP.

Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDA receptor.
Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDA receptor.
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Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDA receptor.
Immunostaining of rabbit retina showing NR2A (1:1000, green) in the rod and cone photoreceptors in the outer plexiform layer as well as the entire inner plexiform layer.

Human, Mouse, Rat
IHC, IP, WB
Rabbit

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Data Sheet
Pooled Serum Pooled Serum

SKU: 1500-NR2A

Size: 10 µg
Price:
$445.00
Ships: 1-2 business days

Product Details

NMDA NR2A Subunit
The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate(NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
IHC, IP, WB
Rabbit
GRIN2A
180 kDa
Fusion protein from the C-terminal region of the NR2A subunit of the rat NMDA receptor.
Human, Mouse, Rat
AB_2492170
Reconstitute in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Lyophilized
Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
Lyophilized
WB: 1:1000
WB Brain: 1:1000
IHC: 1:1000
IP: 3 µl per 200 µg lysate
Unconjugated
Specific for endogenous levels of the ~180 kDa NR2A subunit of the NMDA receptor. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
EPND antibody, FESD antibody, GluN2A antibody, Glutamate [NMDA] receptor subunit epsilon-1 antibody, Glutamate receptor antibody, Glutamate receptor ionotropic N methyl D aspartate 2A antibody, GRIN 2A antibody, GRIN2A antibody, hNR2A antibody, LKS antibody, N methyl D aspartate receptor channel subunit epsilon 1 antibody, N Methyl D Aspartate Receptor Subtype 2A antibody, N methyl D aspartate receptor subunit 2A antibody, N-methyl D-aspartate receptor subtype 2A antibody, NMDA receptor subtype 2A antibody, NMDAR 2A antibody, NMDAR2A antibody, NMDE1_HUMAN antibody, NR2A antibody, OTTHUMP00000160135 antibody, OTTHUMP00000174531 antibody
Q00959
24409

Product Specific References for Applications and Species

  • Immunohistochemistry: Rat
  • Immunoprecipitation: Rat
  • Western Blot: Mouse | Rat
Immunohistochemistry: Rat
PMID Dilution Publication
29339324 1:1000 Aroniadrou-Anderjaska, V., et al. 2018. Oscillatory synchronous inhibition in the basolateral amygdala and its primary dependence on NR2A-containing NMDA receptors. Neuroscience, 373, pp.145-158.
Immunoprecipitation: Rat
PMID Dilution Publication
12536146 3ul per 200ug lysate Alvestad, R.M., et al. 2003. Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. Journal of Biological Chemistry 278:11020-11025.
Western Blot: Mouse
PMID Dilution Publication
37536384not listedLorenz-Guertin, JM, et al. 2023. Inhibitory and Excitatory Synaptic Neuroadaptations in the Diazepam Tolerant Brain. Neurobiology of Disease, 106248.
31235881 1:1000 Pallas-Bazarra, N., et al. 2019. Tau is required for the function of extrasynaptic NMDA receptors. Scientific Reports, 9(1), p.9116.
24166713 1:1000 Pozniak, C.D., et al. 2013. Dual leucine zipper kinase is required for excitotoxicity-induced neuronal degeneration. The Journal of Experimental Medicine, 210(12), 2553-2567.
23325244 1:500-1:1000 Brady, M.L., et al. 2013. Moderate prenatal alcohol exposure reduces plasticity and alters NMDA receptor subunit composition in the dentate gyrus. Journal of Neuroscience, 33(3), pp.1062-1067.
21464302 1:1000 Hicklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS USA 108(16):6650-5.
18647646 not listed Niimi, K., et al. 2008. Improved short-term memory and increased expression of NR2B observed in senescence-accelerated mouse (SAM) P6. Experimental Gerontology, 43(9), pp.847-852.
Western Blot: Rat
PMID Dilution Publication
34284042 1:1000 Nuwer, J.L., et al. 2021. Sustained treatment with an α5 GABA A receptor negative allosteric modulator delays excitatory circuit development while maintaining GABAergic neurotransmission. Neuropharmacology, 197, p.108724.
26842250 1:3000 Goodfellow, M.J., et al. 2016. Neonatal ethanol exposure impairs trace fear conditioning and alters NMDA receptor subunit expression in adult male and female rats. Alcoholism: Clinical and Experimental Research, 40(2), 309-318.
26730408 1:2000 Bashara, H., et al. 2016. Effects of fluoxetine and visual experience on glutamatergic and GABAergic synaptic proteins in adult rat visual cortex. Eneuro, ENEURO-0126.
26706598 not listed O’Leary, H., et al. 2016. Enhanced long term potentiation and decreased AMPA receptor desensitization in the acute period following a single kainate induced early life seizure. Neurobiology of Disease, 87, 134-144.
24166713 1:1000 Pozniak, C.D., et al. 2013. Dual leucine zipper kinase is required for excitotoxicity-induced neuronal degeneration. The Journal of Experimental Medicine, 210(12), 2553-2567.
21464302 1:1000 Hicklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS USA 108(16):6650-5.
12536146 1:1000 Alvestad, R.M., et al. 2003. Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. Journal of Biological Chemistry 278:11020-11025.
11740502 not listed Grosshans, D.R., et al. 2002. LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nature Neuroscience 5:27-33.

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