Our Anti-NSF (N-ethylmaleimide sensitive fusion protein) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat NSF (N-ethylmaleimide sensitive fusion protein) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat striatal lysate showing specific labeling of the ~75 kDa NSF protein.
NSF (N-ethylmaleimide sensitive fusion protein) is a critical component of the SNARE (soluble NSF attachment protein receptors) protein complex that is involved in synaptic vesicle trafficking. Specifically, NSF has been found to be essential in membrane fusion. Furthermore, NSF has been recently demonstrated to bind other protein complexes such as AMPA receptor subunits (GluR2), GATE-16, LMA-1 and Rabs suggesting a more diverse role in the assembly of various protein complexes (Whiteheart et al., 2004).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
NSF
75 kDa
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat NSF, conjugated to keyhole limpet hemocyanin (KLH).
Rat
Human, Non-Human Primate
AB_2492191
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column made with the C-terminal peptide used as antigen.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~75 kDa NSF protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
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Schweizer, F.E., Dresbach, T., DeBello, W.M., O'Connor, V., Augustine, G.J. and Betz, H., 1998. Regulation of neurotransmitter release kinetics by NSF. Science, 279(5354), pp.1203-1206. PMID: 9469810
Matveeva, E.A., Whiteheart, S.W., Vanaman, T.C. and Slevin, J.T., 2001. Phosphorylation of the N-ethylmaleimide-sensitive factor is associated with depolarization-dependent neurotransmitter release from synaptosomes. Journal of Biological Chemistry, 276(15), pp.12174-12181. PMID: 11278345
Whiteheart, S.W. and Matveeva, E.A., 2004. Multiple binding proteins suggest diverse functions for the N-ethylmaleimide sensitive factor. Journal of structural biology, 146(1-2), pp.32-43. PMID: 15037235
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