Our Osteopontin (N-terminal region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Osteopontin (N-terminal region) and is Protein G purified. It is great for use in WB, ICC.
Western blot analysis of human recombinant osteopontin protein (lane 1 & 2) or human MDA-MB-231 cells (lane 3 & 4). The blots were probed with mouse monoclonal anti-Osteopontin (OM5741) at a dilution of 1:1000 (lane 1), 1:4000 (lane 2), 1:250 (lane 3), and 1:1000 (lane 4).
Osteopontin (OPN) is a 34 kDa sialic acid rich member of small integrin-binding ligand N-linked glycoproteins. It is expressed in many different tissues and is post-translationally modified at multiple sites with both glycosylation and phosphorylation. The mature post-translationally modified protein is 60 kDa. OPN is involved with cell survival, proliferation, invasion, and stem like behavior. OPN can interact with CD44, bind hydroxyapatite, and activates many integrins. These interactions are important for OPN function in cell matrix formation. A higher presence of OPN has been found in a variety of cancers, leading to increased tumor growth and metastasis. In addition, OPN is involved in type I immunity through its function as a cytokine where it can enhance production of interferon-gamma and interleukin-12 and reduce production of interleukin-10.
Protein G Purified
Monoclonal
M574
IgG1
ICC, WB
Mouse
SPP1
60
Clone M574 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human osteopontin. This sequence has high homology with rat and mouse osteopontin.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:500
ICC: 1:50
Unconjugated
Clone M574 was purified using Protein G chromatography. The antibody detects a 60 kDa* protein corresponding to the molecular mass of Osteopontin on SDS-PAGE immunoblots of human MDA-MB-231 cells and human full length recombinant Osteopontin protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SSP-1, Urinary stone protein, Uropontin, BNSP, OPN, SSP1, osteopontin
