Anti-p62 (Thr269, Ser272) Antibody

4 Citations

Our Anti-p62 (Thr269, Ser272) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human p62 (Thr269, Ser272) and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of Jurkat cell lysate showing specific immunolabeling of the ~62 kDa p62 phosphorylated at Thr269/Ser272 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).
AD293, A375 and MDA-MB-231 cells were treated with DMSO (control), or MG132 (2 μM), and Doramapimod (50 μM), alone or in combination for 14 h. The whole-cell lysates were subjected to western blot analysis with indicated antibodies. Image from publication CC-BY-4.0. PMID: 35840557
AD293 cells and A375 cells were treated with MG132 (2 μM), and Doramapimod (50 μM), alone or in combination for 14 h. The whole-cell lysates were subjected to western blot analysis with total-SQSTM1, SQSTM1 S403, SQSTM1 T269/S272 (cat. p196-269, 1:1000), and GAPDH. Image from publication CC-BY-4.0. PMID: 37872526.
Western blot of Jurkat cell lysate showing specific immunolabeling of the ~62 kDa p62 phosphorylated at Thr269/Ser272 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).
Western blot of Jurkat cell lysate showing specific immunolabeling of the ~62 kDa p62 phosphorylated at Thr269/Ser272 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).
AD293, A375 and MDA-MB-231 cells were treated with DMSO (control), or MG132 (2 μM), and Doramapimod (50 μM), alone or in combination for 14 h. The whole-cell lysates were subjected to western blot analysis with indicated antibodies. Image from publication CC-BY-4.0. PMID: 35840557
AD293 cells and A375 cells were treated with MG132 (2 μM), and Doramapimod (50 μM), alone or in combination for 14 h. The whole-cell lysates were subjected to western blot analysis with total-SQSTM1, SQSTM1 S403, SQSTM1 T269/S272 (cat. p196-269, 1:1000), and GAPDH. Image from publication CC-BY-4.0. PMID: 37872526.

Human
WB
Rabbit

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Data Sheet
Trial Size Trial Size Pooled Serum Pooled Serum

SKU: p196-269

Volume: 100 µL
1-2 business days
Price:
$430.00

Product Specific References for Applications and Species

Western Blot: Human
PMID Dilution Publication
378725261:1000Zhang, C, et al. 2023. Inhibition of SQSTM1 S403 phosphorylation facilitates the aggresome formation of ubiquitinated proteins during proteasome dysfunction. Cellular & molecular biology letters, 85.
358405571:1000Zhang, C., et al. 2022. Autophagic sequestration of SQSTM1 disrupts the aggresome formation of ubiquitinated proteins during proteasome inhibition. Cell death & disease, 615.
29930081 not listed Zhang, C., et al. 2018. p38δ MAPK regulates aggresome biogenesis by phosphorylating SQSTM1 in response to proteasomal stress. J Cell Sci, Jul 26;131(14).
Western Blot: Mouse
PMID Dilution Publication
26279575 not listed Linares, J.F., et al. 2015. Amino acid activation of mTORC1 by a PB1-domain-driven kinase complex cascade. Cell reports, 12(8), pp.1339-1352.

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