Anti-Pan-Neurofascin Antibody (L11A/41)

Name: Anti-Pan-Neurofascin Antibody
Brand: NeuroMab
SKU: 75-027
Price: 319.0 USD
Availability: InStock

23 Citations

Our Anti-Pan-Neurofascin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L11A/41. It detects human and rat Pan-Neurofascin, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Adult rat optic nerve immunofluorescence staining L11A/41 (5 μg/ml)=red Rabbit anti-Caspr=green Image courtesy of Dr. Matthew Rasband.

Species Reactivity

Human, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-027

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Pan-Neurofascin

Target Description

Neurofascin is encoded by the gene NFASC. Two major isoforms of NeuroFascin are expressed as a result of alternative splicing, NF186 and NF155. These proteins are expressed in the central nervous system. In brain, NF186 is expressed at Nodes of Ranvier andNF155 is expressed in unmyelinated axons. Neuofascin plays a role in cell adhesion and is thought to be involved in processes such as neurite extension, synaptogenesis and myelination. Diseases associated with this gene include Demyelinating Polyneuropathy.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L11A/41

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Nfasc

Molecular Weight

155, 186 kDa (plus additional bands on immunoblot)

Antigen

Fusion protein 1066-1174 (intracellular domain common to NF-155 and NF-186) of rat NF-155 (accession number AAL27854) produced recombinantly in E. Coli

Species Reactivity

Human, Rat

Antibody Registry ID

AB_10672370

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Neurofascin NF-155 NF-186

UniProt Details

UniProt (Human): O94856
UniProt (Immunogen Species): AAL27854

Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Immunohistochemistry: Guinea Pig |Mouse | Rat
Western Blot: Mouse | Rat
Additional Publications: Unspecified

Immunocytochemistry: Human
PMID	Dilution	Publication
24476104	not listed	O'Guin, K.N., et al. 2014. Gas6 enhances axonal ensheathment by MBP+ membranous processes in human DRG/OL promyelinating co-cultures.. ASN Neuro, e00135.

Immunocytochemistry: Rat
PMID	Dilution	Publication
36103253	not listed	Frank, M., et al. 2022. Propofol Attenuates Kinesin-Mediated Axonal Vesicle Transport and Fusion. Molecular Biology of the Cell, ar119.
34731031	not listed	Nabb, A.T., et al. 2022. NgCAM and VAMP2 reveal that direct delivery and dendritic degradation maintain axonal polarity. Molecular Biology of the Cell, .
31461551	not listed	Yang, R., et al. 2019. A novel strategy to visualize vesicle-bound kinesins reveals the diversity of kinesin-mediated transport. Traffic, 851-866.
30089262	1:2 (supe)	Hauser, M., et al. 2018. The Spectrin-Actin-Based Periodic Cytoskeleton as a Conserved Nanoscale Scaffold and Ruler of the Neural Stem Cell Lineage. Cell Reports, 1512-1522.
28426968	not listed	Gumy, L.F., et al. 2017. MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons. Neuron, 347-362.
26888935	1:100	Benned-Jensen, T., et al. 2016. Live Imaging of Kv7.2/7.3 Cell Surface Dynamics at the Axon Initial Segment: High Steady-State Stability and Calpain-Dependent Excitotoxic Downregulation Revealed.. Journal of Neuroscience, 2261-2266.
24711503	1:100	Jones SL, et al. 2014. Axon initial segment cytoskeleton comprises a multiprotein submembranous coat containing sparse actin filaments.. The Journal of Biology, 67-81.
22649224	not listed	Yap, C.C., et al. 2012. Doublecortin (DCX) mediates endocytosis of neurofascin independently of microtubule binding.. Journal of Neuroscience, 7439-7453.
20543823	1:1000	Grubb, M.S., et al. 2010. Activity-dependent relocation of the axon initial segment fine-tunes neuronal excitability.. Nature, 1070-1074.

Immunohistochemistry: Guinea Pig
PMID	Dilution	Publication
21352229	1:100	Shi, Y., et al. 2011. Acrolein induces myelin damage in mammalian spinal cord.. Journal of Neurochemistry, 554-564.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
21838927	1:500	Roza, C., et al. 2011. Accumulation of Kv7.2 channels in putative ectopic transduction zones of mice nerve-end neuromas.. Molecular Pain, .
17610815	not listed	Micheva, K.D., et al. 2007. Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits.. Neuron, 25-36.

Immunohistochemistry: Rat
PMID	Dilution	Publication
32726631	not listed	Holderith, N., et al. 2020. A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses. Cell Reports, .
29444299	1:1000	Stojic, A., et al. 2018. Early Nodal and Paranodal Disruption in Autoimmune Optic Neuritis. Journal of Neuropathology and Experimental Neurology, 361-373.
20466935	1:300	Lorincz A., et al. 2010. Molecular identity of dendritic voltage-gated sodium channels.. Science, 906-909.
18953054	1:100	Lonigro, A., et al. 2009. Disruption of neurofascin and gliomedin at nodes of Ranvier precedes demyelination in experimental allergic neuritis.. Brain, 260-273.
19118165	1:1000	Lorincz, A., et al. 2008. Cell-type-dependent molecular composition of the axon initial segment. . Journal of Neuroscience, 14329-14340.

Western Blot: Mouse
PMID	Dilution	Publication
31910261	not listed	Lee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033.

Western Blot: Rat
PMID	Dilution	Publication
23160985	not listed	Cortes, D.F., et al. 2012. High-capacity peptide-centric platform to decode the proteomic response to brain injury.. Electrophoresis, 3712-3719.
22649224	not listed	Yap, C.C., et al. 2012. Doublecortin (DCX) mediates endocytosis of neurofascin independently of microtubule binding.. Journal of Neuroscience, 7439-7453.

Additional Publications: Unspecified
PMID	Publication
38598291	Mendoza, CS, et al. 2024. Kinesin Regulation in the Proximal Axon is Essential for Dendrite-selective Transport. Molecular Biology of the Cell, ar81.
22473675	Dhaunchak, A.S., et al. 2012. Implication of perturbed axoglial apparatus in early pediatric multiple sclerosis.. Annals of Neurology, 601-613.
17111377	Van Wart, A., et al. 2007. Polarized distribution of ion channels within microdomains of the axon initial segment.. Journal of Comparative Neurology, 339-352.

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Anti-Pan-Neurofascin Antibody (L11A/41)

SKU: 75-027

Product Details

Product Specific References for Applications and Species

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