Anti-Phosphoserine/threonine Antibody (M380A/M380B)

17 Citations

Our Anti-Phosphoserine/threonine mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Anti-Phosphoserine/threonine and is Protein A purified. It is great for use in WB, ICC, IP.

Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1 and 2) then treated with lambda phosphatase (lane 3). The blot was probed with anti-Phosphoserine/threonine mouse monoclonal at 1:250 (lane 1) or 1:1000 (lanes 2 & 3).
Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1 and 2) then treated with lambda phosphatase (lane 3). The blot was probed with anti-Phosphoserine/threonine mouse monoclonal at 1:250 (lane 1) or 1:1000 (lanes 2 & 3).
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Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1 and 2) then treated with lambda phosphatase (lane 3). The blot was probed with anti-Phosphoserine/threonine mouse monoclonal at 1:250 (lane 1) or 1:1000 (lanes 2 & 3).
Immunocytochemical labeling of phosphoserine and phosphothreonine in control and calyculin A-treated A431 cells. The cells were labeled with mouse monoclonal anti-Phosphoserine/threonine (PM3801) and rabbit polyclonal anti-Phosphoserine/threonine (PP2551), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
Western blot showing phosphorylation level (cat PM3801, 1:2000) of NFATC1 in TAZ overexpressed HEK-293T cells after 1 mM Tg treatment or UCHL1 knockout. Image from publication CC-BY-4.0. PMID: 37215988
WT (PKY2255) and pda1Δ (PKY2256) cells expressing Qcr2-GFP were grown in SL medium for the indicated amounts of time, and protein extracts were generated and immunoprecipitated with α-GFP antibody. The immunoprecipitates were then probed with α- phosphoamino acid antibody (cat. PM3801, top panel) and α-GFP antibody (bottom panel). Right panel: day 5 samples were treated overnight with lambda phosphatase or mock-treated. Image from publication CC-BY-4.0. PMID: 37454238
WT (PKY1089) and pda1Δ (PKY1093) cells expressing Mdh1-GFP, as well as the indicated plasmids, were grown in SL medium for the indicated amounts of time, and protein extracts were generated and immunoprecipitated with α-GFP antibody. Phosphorylation of Mdh1-GFP was detected using α-phosphoamino acid antibody (cat. PM3801), and total GFP signal in the immunoprecipitate was observed by immunoblotting with the α-GFP antibody. ϕ denotes an empty vector Image from publication CC-BY-4.0. PMID: 37454238
Mutant lat1Δ cells (PKY2298) expressing the indicated plasmids, as well as Mdh1-GFP, were grown in SL medium for the indicated amounts of time, and protein extracts were generated and immunoprecipitated with with α-GFP antibody. Phosphorylation of Mdh1-GFP was detected using α-phosphoamino acid antibody (cat. PM3801), and total GFP signal in the immunoprecipitate was observed by immunoblotting with the α-GFP antibody. ϕ denotes an empty vector. Image from publication CC-BY-4.0. PMID: 37454238
In vitro kinase assay of nrdD stained with a serine/threonine site-specific antibody (cat. PM3801). Image from publication CC-BY-4.0. PMID: 38683735

Human, Mouse, Rat
ICC, IP, WB
Mouse

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SKU: PM3801

Volume: 100 µL
1-2 business days
Price:
$340.00

Product Details

Anti-Phosphoserine/threonine
Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.
Protein A Purified
Monoclonal
M380A/M380B
IgG1
ICC, IP, WB
Mouse
N/A
N/A
PM3801 Phosphoserine/threonine is a mix of two clones: Clone M380A was generated from a phosphothreonine synthetic peptide (coupled to carrier protein) and Clone M380B was generated.from a phosphoserine synthetic peptide (coupled to carrier protein).
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:500
ICC: 1:50
IP: 1:50
Unconjugated
The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA.
Phosphorylated
pSer/Thr
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Phosphoser/thr mAb
N/A

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
270430841:100Su, KH, et al. 2016. HSF1 critically attunes proteotoxic stress sensing by mTORC1 to combat stress and promote growth. Nature cell biology, 527-539.
Immunoprecipitation: Human
PMID Dilution Publication
301716681:100Malleske, D, et al. 2018. Regulation of Human Airway Epithelial Tissue Stem Cell Differentiation by β-Catenin, P300, and CBP. Stem cells (Dayton, Ohio), 1905-1916.
Western Blot: E. Coli
PMID Dilution Publication
38683735not listedKeskin, BB, et al. 2024. Reverse-Phase Protein Microarrays for Overexpressed Escherichia coli Lysates Reveal a Novel Tyrosine Kinase. Analytical Chemistry, 8721-8729.
Western Blot: Mouse
PMID Dilution Publication
372159881:2000Feng, Z, et al. 2023. The Deubiquitinase UCHL1 Negatively Controls Osteoclastogenesis by Regulating TAZ/NFATC1 Signalling. International Journal of Biological Sciences, 2319-2332.
340315921:1000Charbord, J, et al. 2021. In vivo screen identifies a SIK inhibitor that induces β cell proliferation through a transient UPR. Nature metabolism, 682-700.
33775739not listedFester, L, et al. 2021. Sex neurosteroids: Hormones made by the brain for the brain. Neuroscience letters, 135849.
315619521:1000Su, KH, et al. 2019. Heat Shock Factor 1 Is a Direct Antagonist of AMP-Activated Protein Kinase. Molecular cell, 546-561.
270430841:1000Su, KH, et al. 2016. HSF1 critically attunes proteotoxic stress sensing by mTORC1 to combat stress and promote growth. Nature cell biology, 527-539.
26472556not listedFester, L, et al. 2016. Control of aromatase in hippocampal neurons. The journal of steroid biochemistry and molecular biology, 9-14.
Western Blot: Plant
PMID Dilution Publication
39448885not listedDing, S, et al. 2024. A novel LRR receptor-like kinase BRAK reciprocally phosphorylates PSKR1 to enhance growth and defense in tomato. The EMBO Journal, .
39190354not listedZhu, C., et al. 2024. SlCPK27 cross-links SlHY5 and SlPIF4 in brassinosteroid-dependent photo- and thermo-morphogenesis in tomato. PNAS USA, e2403040121.
38431528not listedZhu, C, et al. 2024. Phosphorylation of sugar transporter TST2 by protein kinase CPK27 enhances drought tolerance in tomato. Plant Physiology, 0.
33711164not listedHu, Z, et al. 2021. The protein kinase CPK28 phosphorylates ascorbate peroxidase and enhances thermotolerance in tomato. Plant physiology, 1302-1317.
Western Blot: Yeast
PMID Dilution Publication
37442609not listedKolitsida, P, et al. 2023. The Pyruvate Dehydrogenase Complex Regulates Mitophagic Trafficking and Protein Phosphorylation. Life Science Alliance, .
Additional Publications: Unspecified
PMID Publication
37322592Song, J, et al. 2023. SlMPK1- and SlMPK2-mediated SlBBX17 Phosphorylation Positively Regulates CBF-dependent Cold Tolerance in Tomato. The New Phytologist, 1887-1902.
36562188Ding, S, et al. 2023. Phytosulfokine peptide optimizes plant growth and defense via glutamine synthetase GS2 phosphorylation in tomato. The EMBO journal, e111858.
36210523Song, J, et al. 2023. SnRK2.4-mediated phosphorylation of ABF2 regulates ARGININE DECARBOXYLASE expression and putrescine accumulation under drought stress. The new phytologist, 216-236.

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