Anti-Phosphoserine/threonine Antibody
Our Anti-Phosphoserine/threonine rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Anti-Phosphoserine/threonine and is antigen affinity purified. It is great for use in WB, ICC, IP.
Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.
Product Specific References for Applications and Species
- Immunocytochemistry: Human
- Immunohistochemistry: Mouse
- Western Blot: Algae | Drosophila | Fungus | Human | Mouse | Plant | Rabbit | Rat
- Additional Publications: Unspecified
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 37494186 | not listed | Yang, D, et al. 2023. Histone H3K79 methylation by DOT1L promotes Aurora B localization at centromeres in mitosis. Cell reports, 112885. |
| 28906490 | not listed | Weng, T, et al. 2017. POPX2 phosphatase regulates apoptosis through the TAK1-IKK-NF-κB pathway. Cell death & disease, e3051. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 38252344 | 1:1000 | Li, Y.B., et al. 2024. Fimbrin associated with Pmk1 to regulate the actin assembly during Magnaporthe oryzae hyphal growth and infection. Stress biology, 5. |
| 32170063 | 1:1000 | Mutlak, YE, et al. 2020. A signaling hub of insulin receptor, dystrophin glycoprotein complex and plakoglobin regulates muscle size. Nature communications, 1381. |
| Western Blot: Algae | ||
| PMID | Dilution | Publication |
| 37036169 | not listed | Levin, G., et al. 2023. A desert Chlorella sp. that thrives at extreme high‐light intensities using a unique photoinhibition protection mechanism. The Plant Journal: For Cell and Molecular Biology, . |
| Western Blot: Drosophila | ||
| PMID | Dilution | Publication |
| 33848457 | 1:1000 | Shindo, Y, et al. 2021. Excess histone H3 is a competitive Chk1 inhibitor that controls cell-cycle remodeling in the early Drosophila embryo. Current biology : CB, 2633-2642. |
| Western Blot: Fungus | ||
| PMID | Dilution | Publication |
| 38690366 | not listed | Wang, J, et al. 2024. The Ser/Thr protein kinase FonKin4-poly(ADP-ribose) polymerase FonPARP1 phosphorylation cascade is required for the pathogenicity of watermelon fusarium wilt fungus Fusarium oxysporum f. sp. niveum. Frontiers in Microbiology, 1397688. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 38632437 | 1:1000 | Zhou, Z, et al. 2024. ULK1-dependent phosphorylation of PKM2 antagonizes O-GlcNAcylation and regulates the Warburg effect in breast cancer. Oncogene, 0. |
| 37951217 | 1:1000 | Long, J, et al. 2023. A combinatorial therapeutic approach to enhance FLT3-ITD AML treatment. Cell reports. Medicine, 101286. |
| 36739326 | 1:1000 | Yuki, R., et al. 2023. SH2D4A promotes centrosome maturation to support spindle microtubule formation and mitotic progression. Scientific Reports, 2067. |
| 33563064 | not listed | Bao, Y., et al. 2021. PRKAA/AMPKα phosphorylation switches the role of RASAL2 from a suppressor to an activator of autophagy. Autophagy, 3607-3621. |
| 33116123 | 1:1000 | Kim, J., et al. 2020. Ketohexokinase-A acts as a nuclear protein kinase that mediates fructose-induced metastasis in breast cancer. Nature communications, 5436. |
| 28906490 | not listed | Weng, T, et al. 2017. POPX2 phosphatase regulates apoptosis through the TAK1-IKK-NF-κB pathway. Cell death & disease, e3051. |
| 25666616 | not listed | Kommaddi, R.P., et al. 2015. Phosphorylation of the deubiquitinase USP20 by protein kinase A regulates post-endocytic trafficking of β2 adrenergic receptors to autophagosomes during …. Journal of Biological Chemistry, 8888-8903. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 39080660 | not listed | Zhu, G., et al. 2024. Glycolytic enzyme PGK1 promotes M1 macrophage polarization and induces pyroptosis of acute lung injury via regulation of NLRP3. Respiratory Research, 291. |
| 38474037 | 1:500 | Herwig, M, et al. 2024. Protein Kinase D Plays a Crucial Role in Maintaining Cardiac Homeostasis by Regulating Post-Translational Modifications of Myofilament Proteins. International Journal of Molecular Sciences, 2790. |
| 38214105 | not listed | Fang, H., et al. 2024. Phytosulfokine promotes fruit ripening and quality via phosphorylation of transcription factor DREB2F in tomato. Plant Physiology, . |
| 37632751 | not listed | Dobson, L, et al. 2023. GSK3 and lamellipodin balance lamellipodial protrusions and focal adhesion maturation in mouse neural crest migration. Cell Reports, 113030. |
| 37363100 | 1:500 | Zhazykbayeva, S., et al. 2023. Oxidative stress and inflammation distinctly drive molecular mechanisms of diastolic dysfunction and remodeling in female and male heart failure with …. Frontiers in Cardiovascular Medicine, 1157398. |
| 36927816 | 1:500 | Mastrototaro, G., et al. 2023. Ablation of palladin in adult heart causes dilated cardiomyopathy associated with intercalated disc abnormalities. Elife, e78629. |
| 36696363 | not listed | Zhao, M., et al. 2023. PGC1α Degradation Suppresses Mitochondrial Biogenesis to Confer Radiation Resistance in Glioma. Cancer Research, 1094-1110. |
| 36278411 | not listed | Aweida, D., et al. 2022. The AAA‐ATPase ATAD1 and its partners promote degradation of desmin intermediate filaments in muscle. EMBO Reports, e55175. |
| 35156314 | not listed | Wang, Y., et al. 2022. Suppression of RIP1 activity via S415 dephosphorylation ameliorates obesity‐related hepatic insulin resistance. Obesity, 680-693. |
| 34558411 | 1:500 | Filomena, M.C., et al. 2021. Myopalladin knockout mice develop cardiac dilation and show a maladaptive response to mechanical pressure overload. Elife, e58313. |
| 33055420 | 1:500 | Michel, K, et al. 2020. C-type natriuretic peptide moderates titin-based cardiomyocyte stiffness. JCI Insight, e139910. |
| 32394454 | 1:500 | Reil, JC, et al. 2020. CaMKII activity contributes to homeometric autoregulation of the heart: A novel mechanism for the Anrep effect. The Journal of physiology, 3129-3153. |
| 30061109 | not listed | Aweida, D, et al. 2018. GSK3-β promotes calpain-1-mediated desmin filament depolymerization and myofibril loss in atrophy. The Journal of cell biology, 3698-3714. |
| 28915917 | not listed | Unger, A, et al. 2017. Translocation of molecular chaperones to the titin springs is common in skeletal myopathy patients and affects sarcomere function. Acta neuropathologica communications, 72. |
| 27841876 | 1:500 | Eisenberg, T., et al. 2016. Cardioprotection and lifespan extension by the natural polyamine spermidine. Nature Medicine, 1428-1438. |
| 27814504 | not listed | Liu, J., et al. 2016. Inflammation improves glucose homeostasis through IKKβ-XBP1s interaction. Cell, 1052-1066. |
| Western Blot: Plant | ||
| PMID | Dilution | Publication |
| 39056291 | not listed | Yan, Y., et al. 2024. A distinct protein posttranslational modifications-linked OsATL32-OsPPKL2-OsGSK2 loop modulates rice immunity against blast disease. The New Phytologist, . |
| 38833610 | 1:1000 | Yu, WW, et al. 2024. The calcium-dependent protein kinase CPK16 regulates hypoxia-induced ROS production by phosphorylating the NADPH oxidase RBOHD in Arabidopsis. The Plant cell, . |
| 38735686 | not listed | Yu, Y, et al. 2024. A transthyretin-like protein acts downstream of miR397 and LACCASE to regulate grain yield in rice. The Plant cell, . |
| 38670968 | not listed | Zhou, N, et al. 2024. RinRK1 enhances NF receptors accumulation in nanodomain-like structures at root-hair tip. Nature Communications, 3568. |
| 38561315 | not listed | Diao, Z, et al. 2024. Functional screening of the Arabidopsis 2C protein phosphatases family identifies PP2C15 as a negative regulator of plant immunity by targeting BRI1-associated receptor kinase 1. Molecular Plant Pathology, e13447. |
| 38309260 | not listed | Qi, F., et al. 2024. DGK5β-derived phosphatidic acid regulates ROS production in plant immunity by stabilizing NADPH oxidase. Cell Host & Microbe, . |
| 37977141 | not listed | Wang, R, et al. 2023. Reversible phosphorylation of a lectin-receptor-like kinase controls xylem immunity. Cell host & microbe, 2051-2066. |
| 35643603 | not listed | Yao, H, et al. 2022. Binding of 14-3-3κ to ADF4 is Involved in the Regulation of Hypocotyl Growth and Response to Osmotic Stress in Arabidopsis. Plant Science: An International Journal of Experimental Plant Biology, 111261. |
| 36808553 | 1:1000 | Leng, J., et al. 2023. ARBUSCULAR MYCORRHIZA-INDUCED KINASES AMK8 and AMK24 associate with the receptor-like kinase KINASE3 to regulate arbuscular mycorrhizal …. The Plant Cell, 2006-2026. |
| 35851623 | 1:500 | Li, Y., et al. 2022. Plasma membrane-nucleo-cytoplasmic coordination of a receptor-like cytoplasmic kinase promotes EDS1-dependent plant immunity. Nature Plants, 802-816. |
| 35185997 | not listed | Yue, Z.L., et al. 2022. Overexpression of Lectin Receptor-Like Kinase 1 in Tomato Confers Resistance to Fusarium oxysporum f. sp. Radicis-Lycopersici. Frontiers in Plant Science, 836269. |
| 35099562 | 1:1000 | Shi, H., et al. 2022. BRASSINOSTEROID-SIGNALING KINASE1 modulates MAP KINASE15 phosphorylation to confer powdery mildew resistance in Arabidopsis. The Plant Cell, 1768-1783. |
| 33619522 | 1:500 | Yang, Q., et al. 2021. The receptor-like cytoplasmic kinase CDG1 negatively regulates Arabidopsis pattern-triggered immunity and is involved in AvrRpm1-induced RIN4 phosphorylation. Plant Cell, 1341-1360. |
| 33037150 | 1:1000 | Wang, P., et al. 2020. The cotton wall-associated kinase GhWAK7A mediates responses to fungal wilt pathogens by complexing with the chitin sensory receptors. Plant Cell, 3978-4001. |
| 32487563 | 1:2500 | Ganguly, A., et al. 2020. FRA1 modulates cortical microtubule localization of CMU proteins. Plant Cell, 2508-2524. |
| 31830443 | 1:500 | Gong, BQ, et al. 2019. Cross-Microbial Protection via Priming a Conserved Immune Co-Receptor through Juxtamembrane Phosphorylation in Plants. Cell host & microbe, 810-822. |
| 30131419 | not listed | Xu, S, et al. 2018. Tomato PEPR1 ORTHOLOG RECEPTOR-LIKE KINASE1 Regulates Responses to Systemin, Necrotrophic Fungi, and Insect Herbivory. The Plant cell, 2214-2229. |
| 29396039 | not listed | Liu, J, et al. 2018. A Tyrosine Phosphorylation Cycle Regulates Fungal Activation of a Plant Receptor Ser/Thr Kinase. Cell host & microbe, 241-253. |
| 29396039 | not listed | Liu, J, et al. 2018. A Tyrosine Phosphorylation Cycle Regulates Fungal Activation of a Plant Receptor Ser/Thr Kinase. Cell host & microbe, 241-253. |
| Western Blot: Rabbit | ||
| PMID | Dilution | Publication |
| 22427066 | 1:1000 | Phoungpetchara, I., et al. 2012. Expression of the male reproduction-related gene (Mar-Mrr) in the spermatic duct of the giant freshwater prawn, Macrobrachium rosenbergii. Cell Tissue Research, 609-623. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 38656649 | 1:500 | Kovács, Á, et al. 2024. Sex-specific cardiovascular remodeling leads to a divergent sex-dependent development of heart failure in aged hypertensive rats. GeroScience, 0. |
| 35584900 | 1:500 | Gömöri, K., et al. 2022. Ca2+/calmodulin‐dependent protein kinase II and protein kinase G oxidation contributes to impaired sarcomeric proteins in hypertrophy model. ESC Heart Failure, 2585-2600. |
| 34208541 | 1:500 | Kovács, Á, et al. 2021. Interventricular Differences of Signaling Pathways-Mediated Regulation of Cardiomyocyte Function in Response to High Oxidative Stress in the Post-Ischemic Failing Rat Heart. Antioxidants (Basel, Switzerland), 964. |
| 33295687 | 1:500 | Cuijpers, I, et al. 2021. Linagliptin prevents left ventricular stiffening by reducing titin cleavage and hypophosphorylation. Journal of cellular and molecular medicine, 729-741. |
| 32523538 | 1:500 | Kolijn, D, et al. 2020. Enhanced Cardiomyocyte Function in Hypertensive Rats With Diastolic Dysfunction and Human Heart Failure Patients After Acute Treatment With Soluble Guanylyl Cyclase (sGC) Activator. Frontiers in physiology, 345. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 36240763 | Ma, M., et al. 2022. A surface-receptor-coupled G protein regulates plant immunity through nuclear protein kinases. Cell Host and Microbe, 1602-1614. | |