Our PKCα (Ser-657/Tyr-658) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, rabbit, and rat PKCα (Ser-657/Tyr-658) and is antigen affinity purified. It is great for use in WB, ICC, IP.
Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKCα antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKCα (Central region) or anti-phospho-PKCα (Ser-657/Tyr-658). The latter shows no detection of PKCα after phosphatase treatment.
The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKCα and PKCβI respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.
Antigen Affinity Purified
Polyclonal
IgG
ICC, IP, WB
Rabbit
PRKCA
82
Phospho-PKCα (Ser-657/Tyr-658) synthetic peptide corresponds to amino acid residues around serine 657 and tyrosine 658 of human PKCα. This sequence is similar to the conserved sites in rat and mouse, and has high homology to dual sites in human, rat, and mouse PKCβI (Ser-661/Tyr-662) and PKCγ (Thr-674/Tyr-675).
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:100
IP: 1:100
Unconjugated
This antibody detects an 82kDa* protein corresponding to the molecular mass of phosphorylated PKCα on SDS-PAGE immunoblots of neonatal rat brain lysate. Similar results were observed in human SKN-SH, endothelial, and HeLa cells, as well as rabbit spleen fibroblasts and rat pituitary cells. In immunoprecipitation experiments with various PKC isoforms, this antibody detected PKCα and PKCβ but not other PKC isoforms in rat brain lysate.
Phosphorylated
S657/Y658
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
